Sentences with phrase «expand cultured cells»

«Our results suggest that megakaryocytes might be used clinically to facilitate adult stem cell regeneration and to expand cultured cells for adult stem cell transplants,» says Meng Zhao, Ph.D., a postdoctoral fellow at Stowers and lead author on the study.

Not exact matches

The technology substantially expands our ability to change gene expression in cultured cells and animals..»

Efforts to obtain and study genomes and enrichment cultures of uncultivated microbial lineages will likely further expand our knowledge about archaeal phylogenetic and metabolic diversity and their cell biology and ecological function.

In a typical 3D culture system, cells cling to each other, forming spheroids that float in liquid culture media or expand into a soft matrix.

Their study, which appears as a letter in the Sept. 8 edition of Nature Medicine, expands on work published in April showing that a combination of ribavirin and interferon - alpha 2b stops MERS - CoV from replicating in cell culture.

After collecting biopsies from banked human donor eyes, the team expanded the numbers of cells in a culture plate using human serum to nourish them.

miR - 34a - deficient embryonic stem cell culture is enriched with cells with expanded developmental potential as shown by their strong MuERV - L induction (red).

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

Further, when TILs have been used for such purposes, the proportion of what Harari calls the «juiciest» T cells — those recognizing mutations on cancer cells — tends to decline significantly when the cells are expanded in culture.

GenIbet offers 1000 m2 of GMP - compliant upstream and downstream manufacturing facilities, including QC labs and R&D facilities, three BSL2 units for the cultivation of bacterial cells (50 L capacity), insect and mammalian cells (50 L capacity for suspension cell cultures, 168,000 cm2 capacity for adherent cells) and the propagation of viruses (50 L capacity, with space to expand up to 200 L).

We concluded that an expanded Revive & Restore, in partnership with biotech organizations, will be able to offer services that include Genetic Insight (DNA sequencing and analysis), Biobanking (tissue collection and cell culturing), Advanced Reproductive Services (cloning and germ - line transmission), and Genome Engineering (CRISPR, gene - drive, etc.) Applications include restoring genetic diversity, augmenting adaptation to climate change, conferring resistance to disease, and extirpating harmful invasive species.

«This new understanding of the role played by scrawny may make it easier to expand stem cell populations in culture, and to direct stem cell differentiation in desired directions,» says Spradling.

Clinical trials use either Stromal Vascular Fraction (SVF), or the cells are Culture Expanded.

In a previous study by the same group 78, autologous culture - expanded MSC were infused to breast cancer patients to investigate whether MSC would enhance the engraftment of peripheral blood stem cells after myeloablative therapy.

Taken together, these data indicate that hiPS cells expanded in the DEF ‑ CS culture system can be successfully edited using gesicles while retaining their pluripotency.

hiPS cells can be cultured, edited, and clonally expanded using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

Delaney et al. [4] who have shown that cord blood - derived CD34 + cells cultured in StemSpan ™ medium with cytokines, on plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.

In order to modify or expand primary human hematopoietic cells, they must first be isolated from the body and then cultured under conditions that allow for their modification.

Another example is AMDC — autologous adult skeletal muscle primary cells that are expanded in culture prior to injection and are tested for the treatment of ischemic heart failure.

By culturing cells in a fed - batch system with StemSpan ™ medium containing cytokines and UM171, expanded cells were found to successfully engraft and repopulate immunocompromised mice, with no disadvantage when compared to unmanipulated cells.

The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane within a microfluidic device with human intestinal microvascular endothelium cultured in a parallel microchannel under flow and cyclic deformation.

December 21, 2016 — Noteworthy NIH advances in basic research include an expanded map of the human brain, nanoparticles that convert white fat to calorie - burning brown fat, and a 3 - D cell culture model of the placenta.

Last year, this team reported recurrent changes in the genomes of human pluripotent stem cells as they are expanded in culture.

Advanced reproductive techniques such as artificial insemination, culturing of primordial germ cells for germ - line transmission, stem cell embryogenesis, and cloning offer an array of new ways to expand and even infuse lost and new genetic variability into at - risk wildlife populations.

However, we have not attempted to culture the more endodermal V+S − cells and it will be interesting to see if these cells can be expanded in vitro.

Non-adherent cells are removed after overnight culture and adherent populations are expanded under low density conditions (seeding density 1000 cells / cm2 and allowed to grow upto 60 — 70 % confluency).

Here we report that cultures of expanded potential stem cells can be established from individual eight - cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells.

In the November 6 Nature Methods, the team led by Bing Lim and Massimo Nichane of the Genome Institute of Singapore and Kyle M. Loh of Stanford University School of Medicine, and including Siva V, Ph.D., of The Jackson Laboratory's Single Cell Biology Lab, showed that mouse Sox9 + multipotent embryonic lung progenitors can be isolated and expanded long - term in 3D culture.

For this reason, investigators are expanding these cells in culture to obtain numbers in the millions.

Unfortunately, we found that a soluble form of CD40L was not effective at activating and expanding canine B cells from peripheral blood lymphocytes in culture.