Sentences with phrase «experiments with human cells»

In experiments with human cells, Shengdar Tsai of Massachusetts General Hospital and Harvard Medical School found that some guide RNAs nearly always lead Cas9 to the correct cutting site.

As I stated in my original article, prior to conducting experiments with human cells, ANT - OAR techniques would need to be rigorously tested in animal models to establish a procedure that guarantees with reasonable certainty that an embryo is not generated.

In one experiment with human cells, a guide RNA should have led the Cas9 enzyme only to a gene on chromosome 2 (yellow bar), but it also directed the enzyme to many off - target sites (red) on several other chromosomes.

Experimenting with human cells and mice, Johns Hopkins researchers have found that a genetic mutation that alters a protein called NOD1 may increase susceptibility to human cytomegalovirus (CMV) infection.

Not exact matches

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

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Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

Scientists have long experimented with organs - on - chips: tiny representations of human organs, such as lungs, hearts and intestines, made from cells embedded on plastic about the size of a computer memory stick.

He had been experimenting with human cancer cells, microbes, and mothers» milk.

Working with human breast cancer cells and mice, scientists at The Johns Hopkins University say new experiments explain how certain cancer stem cells thrive in low oxygen conditions.

This image depicts a single cell experiment in which a human neutrophil was picked up by a micropipette (top panel) and brought into close contact with S. Typhimurium cells immobilized by optical tweezers.

For the purpose of additional experiments, the researchers generated myocardial cells from embryonic stem cells and human skin cells, in collaboration with the lab headed by Prof Dr Jürgen Hescheler at the University of Cologne.

All of these findings were supplemented with several other experiments that were designed to learn how CHI3L1 interacts with other cells involved in the tissue repair response in both human and mouse lungs.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

In experiments involving mice injected with human GBM cells, the scientists also demonstrated that if either PTEN or DAXX were eliminated, then tumor growth occurred.

Most experiments with human stem cells have only shown that the cells can acquire the phenotype of various differentiated cell types, but have not shown that the cells are functional at the transplantation site.

In vitro experiments used human colon carcinoma cells with and without MMP9 and mouse embryonic fibroblasts, which are connective tissue cells that make the extracellular matrix and collagen and play an important role in tissue repair.

Antoine E. Karnoub, another researcher at Whitehead, performs experiments on living human and mouse cells that are kept in a freezer packed with liquid nitrogen.

Experiments on mice and on heart cells obtained from infants born with congenital heart disease suggest that neuregulin 1, a human growth factor, can put infant heart cells on a path that mimics normal growth rather than stalling out.

Their experiments with lab - grown mouse and human T - cells suggest that people with cancer who have a greater variety of such receptors may respond better to immunotherapy drugs and vaccines.

His team have begun recolonising the primate scaffolds with human cells that line blood vessels, the first step towards human - scale biolimb development, and have started experiments using human myoblasts in rats instead of the mice ones.

It is especially important, he says, to close the loophole that leaves unregulated experiments with animal embryos that contain human cells.

They performed a series of experiments with alginate barriers surrounded by human mammary cells, observing how the cells migrate when the barrier is dissolved away.

To explore the potential for application, the group then attempted a similar experiment using human blood stem cells taken from umbilical cords, which they transfected with a vector encoding human Id3.

The experiment's final product is equivalent to the naturally occurring genetic code of M. genitalium, with two minor exceptions: The scientists disabled the gene that gave the bug power to infect human cells, and they added a few «watermarks,» short strips of signature genetic code that identify the product as man - made.

Further experiments in human brain cells called astrocytes, which are targeted by CMV, revealed a 100-fold decrease in the amount of virus present when they were treated with valnoctamide (Journal of Neuroscience, DOI: 10.1523 / jneurosci.0970 - 17.2017).

Mice with human astrocytes performed better on memory experiments than those that had received mouse cell grafts, the team reports today in Cell Stem Ccell grafts, the team reports today in Cell Stem CCell Stem CellCell.

In another set of experiments using the fly and human stem cells, the scientists added antisense oligonucleotides, bits of RNA designed to bind to the repetitive RNA strands, blocking them from interacting with the RanGAP protein.

But in their experiments with both fly and human brain cells made from patients with the ALS - associated C9orf72 mutation, Rothstein and Lloyd discovered RanGAP is clumped up outside the nucleus.

In another experiment, treating human brain cancer cells containing FGFR3 - TACC3 with mitochondrial inhibitors interrupted the production of energy inside cancer cells and significantly slowed tumor growth.

The experiments often require drastic steps (such as knocking out genes, killing cells with antibodies, sewing animals together) that are impossible in human beings.

That and other concerns led the National Institutes of Health to announce in 2015 that it would not fund experiments that put human pluripotent stem cells, those with the ability to morph into almost any kind of tissue or organ, into the early embryos of other animals.

In separate experiments reported in Nature — one with mice, the other transplanting human stem cells into mouse bone marrow — researchers demonstrated techniques with the potential to produce all types of blood cells.

After having initial success using CRISPR therapy with mice, he has moved on to inserting human stem cells into monkeys for his experiments.

TIMP - 1, previously described as being able to protect against cytokine and STZ ‐ induced β cell death [5, 6], was one of the most enriched factors in co-culture experiments using mouse and human islet cells, and the authors found that TIMP - 1 was induced by pro-inflammatory factors which are commonly associated with T1DM.

By examining the results of genome - wide association studies (GWAS) in conjunction with experiments on mouse and human red blood cells (RBCs), researchers in the lab of Whitehead Institute Founding Member Harvey Lodish have identified the protein cyclin D3 as regulating the number of cell divisions RBC progenitors undergo, which ultimately affects the resulting size and quantity of RBCs.

The start of Phase II coincided with the relocation of I - Stem in 1600 square meters, gradually extended to 2300, located within theGenopole campus I. Research space consists mainly pf confined Level II areas required for work on human cells and unconfined laboratories for biochemical experiments, molecular biology, histology and analyses.

In a series of lab experiments with cell lines, human xenograft tumors in mice and primary human prostate cancer samples, the researchers demonstrated that miR - 34a inhibits prostate cancer stem cells by suppressing CD44.

But MPs including Gordon Brown and David Cameron, who both have young children with severe medical conditions, agreed on a free vote that the benefits of stem cell research using embryos were such that experiments, including the use of human admixed embryos, should be allowed.

«Theranostics» Simultaneously Kill and Image Prostate Cancer Cells Experimenting with human prostate cancer cells and mice, cancer - imaging experts developed a method for finding and killing malignant cells while sparing healthy Cells Experimenting with human prostate cancer cells and mice, cancer - imaging experts developed a method for finding and killing malignant cells while sparing healthy cells and mice, cancer - imaging experts developed a method for finding and killing malignant cells while sparing healthy cells while sparing healthy ones.

In collaboration with Nanyang Technological University, experiments were also conducted to co-deliver paclitaxel and small interfering RNA (siRNA) targeting a protein that prevents cell death (Bcl - 2) to MDA - MB - 231 human breast cancer cell line.

Initially, Novocell experimented with implanting micro-encapsulated human islet cells.

Howard Stark (Dominic Cooper) is aligned with Dr. Abraham Erskine (Stanley Tucci doing his best Dr. Strangelove) in a secret government experiment to perfect cell regeneration in the human body and beat Hitler in the «Supermen» race.

High has experimented with transgenics in a number of multimedia, interspecies projects such as Embracing Animal (2004 - 2006), and she has staged competitions between human white blood cells through her ongoing Blood Wars tournaments.