Sentences with phrase «expressing cell lines»

Negative, intermediate and / or strong protein expressing cell lines cores verified using Quantitative Digital Pathology (QDP)

Defined human cell line cores containing positive and negative protein expressing cell lines on the same slide

Persomics now provides CRISPR based screening tools for CAS9 expressing cell lines, and we are currently working to extend this.

Not exact matches

The scientists also generated a panel of (reconstructed) ancestral and existing TRIM5 genes (19 total), expressed them in cultured cell lines, and exposed the cells to 16 different retroviruses (lentiviruses and others) to see which TRIM5 versions conferred resistance to which viruses.

Luster's team hopes to further investigate the characteristics of joints that underlie the critical role of C5a in initiating type III hypersensitivity and whether specific molecules expressed on endothelial cells lining joints play a role in transporting C5a and chemokines from the joint space into adjacent blood vessels.

They found that infection of the narrowest airways of the lung by PIV3 alone caused moderate levels of inflammation, but after infection by PIV3 expressing RSV NS2, the epithelial cells lining the narrow airways were shed rapidly into the airway lumen.

«But what we've now shown is that RSV has an increased ability to cause airway obstruction because, during an RSV infection, the virus expresses a specific RSV - encoded non-structural protein, or NS2, in epithelial cells, causing the cells to shed from the airway lining and into the airway lumen.

Stem cell advocates have been expressing serious worry that ethical requirements spelled out in the draft guidelines — in particular, informed consent procedures for embryo donors — will rule out the use of many existing human embryonic stem cell lines, including the 21 lines approved under the Bush Administration.

In one study, geneticist Joseph Ecker at the Salk Institute in California took various stem cell lines reprogrammed from skin, fat, and other tissues and examined each line's genome for dna methylation, chemical marks that alter how genes are expressed.

In combination with a separate technique that measures the degree to which a gene is expressed, the researchers then identified genes that were either mutated or functionally altered through expression levels in these bladder cancer cell lines.

The goal of the first experiment was to see whether lncRNAs are differentially expressed in prostate cancer by measuring total RNA from prostate cancer cell lines and normal epithelial prostatic cells using NCode human ncRNA array and SurePrint G3 human lncRNA microarrays.

Unlike OCT4, these genes can only be studied in human embryos because they are not expressed the same way, or at all, in mouse embryos or immortalized lines of human stem cells, says her colleague Robin Lovell - Badge, also at the Crick Institute.

Congressional members, leaders at the National Institutes of Health, and a variety of patient groups have expressed increasing dissatisfaction with the president's policy as the limitations of existing cell lines — 22 of which are available — have become clear.

To overcome this, researchers from the University of Texas Southwestern Medical Center and Johns Hopkins University stabilized pri - miRNAs by expressing a dominant negative form of DROSHA, the RNase III enzyme responsible for pri - miRNA cleavage, in a variety of human and mouse cell lines.

Using a new TaqMan PCR - based technique, the researchers screened a number of cancer cell lines from various tissues, and discovered that tRNA halves were specifically expressed in large quantities in sex hormone - dependent cancers, i.e., estrogen receptor (ER)- positive breast cancer and androgen receptor (AR)- positive prostate cancer that are driven by the hormones estrogen and testosterone.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

Thus, our selection strategies, which were based on targeting endothelial populations that expressed both of these inducible glycoproteins, suggest that the endothelial cell lines generated in this study are most likely from this microcirculatory region.

To evaluate coreceptor tropism, a representative Env from the X4 - ZFN mice and the viral innoculum were pseudotyped and used to infect NP2 cell lines expressing CD4 and either CCR5 or CXCR4.

As full length Bk132 Env would not pseudotype on an NL43 HIV core we truncated the cytoplasmic tail of the Envs [49], [50], and conducted tropism testing on NP2 cell lines expressing CD4 with either CCR5 or CXCR4.

Pharmacology of human dopamine D3 receptor expressed in a mammalian cell line: Comparison with D2 receptor

We perform the transient silencing of several genes in parallel by RNA interference in order to identify those able to modify two types of DM1 biomarkers expressed by one DM1 - hES cell line progeny: the pathological intranuclear ribonucleoaggregates called foci and the insulin receptor splicing defect.

Indeed, human pluripotent stem cell lines and derivatives, which express a disease - related mutated gene, represent a relevant and pertinent disease cell model to analyse pathological mechanisms through large - scale drug screenings.

The example shown reveals that these estrogen agonists show the strongest connectivity to each other in MCF7, a human breast cancer cell line that expresses the estrogen receptor.

They utilized the mouse line in which GFP is expressed in cells, but have an active TH promoter.

Normalized (QNORM): The shape of the expression profile for each cell line is normalized such that all of the data for the collection is on the same scale (4 as minimally / not expressed, 15 as a maximum readout).

TNA3: BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.

Cell lines express endogenous or recombinant proteins and are available in a ready - to - use format to facilitate your research efforts

The parent TZM - bl cell line is an engineered HeLa cell clone that expresses human CD4, CCR5 and CXCR4 and contains HIV - 1 Tat -

This live cell assay features a cell line expressing the innate immune receptor, Toll - like receptor - 2 (TLR2).

To identify relevant P450s, the researchers created 27 different insect cell lines, each expressing a unique honeybee P450 gene.

When researchers express interest in obtaining a certain knockout mouse line, the repositories will send them live mice, frozen embryos, sperm, and / or ES cells, so they can study the mice in their own labs.

The parent TZM - bl cell line is an engineered HeLa cell clone that expresses human CD4, CCR5 and CXCR4 and contains HIV - 1 T

Albumin and α1AT are well - expressed in enhanced hiPS - HEP cells from all three hiPS cell lines (Figure 3, Panels A, B, and C), as shown by immunostaining and mRNA expression analysis.

The parent TZM - bl cell line is an engineered HeLa cell clone that expresses human CD4, CCR5 and CXCR4 and contains HIV - 1 Ta

In cytotoxicity assays employing PPAM, we found no significant differences of IC50 values between SCLC tumor cells, SCLC CTC lines which express the Wnt pathway and normal cells such as HEK293 (data not shown).

First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were cultured using the DEF ‑ CS culture system in a 48 - well plate.

Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest.

Jurkat cell line expressing the HIV - 1 tat protein.

This is a derivative of the TZM - bl cell line, engineered by lentiviral transduction to stably express human FcγRIIb on the surface.

This is a derivative of the TZM - bl cell line, engineered by lentiviral transduction to stably express human FcγRI on the surface.

In our experiments, both HNF4α and ASGPR1 are well - expressed in enhanced hiPS - HEP cells from all three hiPS cell lines (Figure 2), as shown by homogeneous nuclear staining for HNF4α and homogeneous membrane staining for ASGPR1.

This is a derivative of the TZM - bl cell line, engineered by lentiviral transduction to stably express human FcγRIIIa on the surface.

Lentiviral vectors are also commonly used to make stable transgene - expressing or knockout cell lines.

To investigate if AKT activation is sufficient to activate the mTOR pathway in ALK + ALCL cells, we infected the Karpas 299 and SU - DHL1 cell lines with an adenoviral vector, adeno - myrAkt, expressing constitutively active AKT (23).

We have established stably transfected HEK 293 cell lines expressing high levels of functional human ether - a go - go - related gene (HERG) channels.

For the 374 expressed genes, we find many regions with statistically significant association of single nucleotide polymorphisms (SNPs) with expression variation in lymphoblastoid cell lines after correcting for multiple tests.

Pim - 3, a Proto - Oncogene with Serine / Threonine Kinase Activity, Is Aberrantly Expressed in Human Pancreatic Cancer and Phosphorylates Bad to Block Bad - Mediated Apoptosis in Human Pancreatic Cancer Cell Lines

The donor luciferase enzyme can be expressed on the N - terminus of the receptor of interest in an appropriate transfected cell line.

Treatment of the Gb3 - expressing gastric carcinoma cell line St3051 with STxB coupled to SN38, the active metabolite of the topoisomerase type I inhibitor irinotecan, resulted in > 100-fold increased cytotoxicity, as compared with irinotecan alone.

The researchers next assessed the function of the NK cells to inhibit HIV replication using the CEM - GFP T - cell line which when infected with HIV - 1 expresses GFP.