Sentences with phrase «expressing cells show»

Not exact matches

Because the human cells had been genetically engineered to express green fluorescent protein, the tiny blobs showed up in brilliant lime through the transparent window that the scientists glued into the mice's skull.

The hair cells also expressed a green protein, showing they had taken up the gene.

Previous research shows human induced pluripotent stem cells (hiPSCs)-- generated directly from adult cells — can express markers for a wide variety of cells, including those that secrete NP.

Since genes for the T - cell receptor beta chain were previously shown to be on mouse chromosome 6, all three of the Ig - like multigene families expressed and rearranged in T cells are located on different chromosomes, just as are the B - cell multigene families for the Ig heavy chain, and the Ig kappa and lambda light chains.

«We have been able to show that this protein is highly expressed in metastatic triple - negative breast cancer, and when we are depleting it from triple - negative breast cancer cells, we found that the cancer cells are not metastasizing,» Dr. Paul said.

«We have shown that it is now possible to accurately design complex, multipass transmembrane proteins that can be expressed in cells.

The scientists have shown that, in all cancers, a sort of «identity crisis» is observed in cancerous cells: in the organs or tissues in which a tumor develops, genes specific to other tissues or to other stages of the development of the organism express themselves in an aberrant manner.

Left: A fruit fly showing location of brain pacemaker cells that express rhodopsin 7.

A team of researchers led by Rodrigo Lacruz, MSc, PhD, assistant professor in the Department of Basic Science and Craniofacial Biology at NYU College of Dentistry, has published a paper in Scientific Reports (5:15803) titled «Dental enamel cells express functional SOCE channels,» which reports the results of a study showing for the first time the mechanism of calcium transport essential in the formation of dental enamel.

We show here that acidic mammalian chitinase (AMCase) is induced via a T helper - 2 (Th2)-- specific, interleukin - 13 (IL - 13)-- mediated pathway in epithelial cells and macrophages in an aeroallergen asthma model and expressed in exaggerated quantities in human asthma.

Previous studies showed that cells which express the same type of cadherin interact with each other, but cells with different types of cadherins remain separate.

«But what we've now shown is that RSV has an increased ability to cause airway obstruction because, during an RSV infection, the virus expresses a specific RSV - encoded non-structural protein, or NS2, in epithelial cells, causing the cells to shed from the airway lining and into the airway lumen.

We should not only question the construct of the «ethics of curing,» as I will show, but we also need to look critically at reservations toward research on embryonic stem cells as they are expressed in our society.

Wellcome Trust Sanger Institute scientists and their collaborators have developed a new analysis tool that was able to show, for the first time, which genes were expressed by individual cells in different genetic versions of a benign blood cancer.

They showed in previous studies with cell cultures that when adding RK - 33 to malignant lung and other cancerous cells that highly express DDX3, proliferation slowed or halted, and the cells» ability to form colonies was impaired.

«This study shows us that these TCR specific T cells are safe and feasible in patients expressing NY - ESO - 1,» he said.

This shows fat accumulation (green) in human skeletal muscle cells (red) that either express BRCA1 (left) or do not express BRCA1 (right).

The team went on to show that supplementing heavy stable isotope - labeled forms of these precursors led to incorporation of heavy amino acids into proteins produced in enzyme expressing cells.

These are images of mouse embryonic stem cells which grow in a round colony of cells (A) and express Sox2 (B), shown in red.

The current study showed that GARP was expressed upon activation of B cells and may therefore act as an important checkpoint for B cell tolerance.

«Sumudu has shown that liver cells that express high levels of the IFITM proteins are resistant to infection with the hepatitis C virus by blocking entry of the virus into the cells,» Associate Professor Beard says.

Previous evidence for a breast cancer link has been mixed — one study found increased risk in women exposed before age 14, whereas others found no association — but in a lab dish, DDT has been shown to activate the HER2 gene in human breast cells, which is expressed in some breast cancers.

Results showed that not only does anti-androgen receptor therapy reduce the ability of androgen - receptor - expressing triple - negative breast cancers to proliferate, migrate and invade, but for these cells, androgen receptor seems essential to survival.

Experiments with mouse embryo support cells that express mutant DUB or pseudo-DUB proteins show an impaired immune response when infected with a virus and impaired DNA damage repair when exposed to ionizing radiation, further validating the need for complex's correct structure.

The researchers showed that the iPS cell teratomas expressed high levels of certain genes that could trigger immune cells to attack.

The researchers were able to show that this receptor, which is expressed in inhibitory interneurons (cells that regulate excitement in order to avoid potentially pathological cerebral over activity), was indispensable in order for neurons to find their correct location in the developing cortex.

B, Histograms of GFP and P2X3 normalized immunoreactivity of individual cells showing that whereas the P2X3 staining shows a unimodal normal distribution (mean 0.58), the GFP population is bimodal suggestive of high - expressing (mean = 0.69) and low - or nonexpressing populations (mean 0.12).

E, Histograms of GFP and FG normalized fluorescence intensity of individual cells showing bimodal distributions suggestive of a high - expressing population (mean GFP: 0.59, FG: 0.62) and a nonexpressing population (mean GFP: 0.08, FG: 0.08).

Remarkably, the relative expression levels of all marker genes were only slightly decreased when R206H ACVR1 and Q207D caACVR1 cultures were cotransfected with Noggin, whereas cells expressing the wild - type ACVR1 or empty viral vector showed clear downregulation of all analyzed marker genes for chondrogenesis.

We showed previously that myostatin circulates in the blood and that systemic effects can be achieved by implanting myostatin - expressing cells into a single site in the lower limb [21]; however, there have been no experiments that have demonstrated conclusively that myostatin normally acts systemically.

Dr. Rossi has shown that with the expression of RAD52, a protein not usually expressed in HDR, and dn53BP1, an altered form of 53PB1 (regularly found in the NHEJ pathway), he was able to enable efficient HDR at multiple points of the genome in patient - derived induced pluripotent stem cells.

The example shown reveals that these estrogen agonists show the strongest connectivity to each other in MCF7, a human breast cancer cell line that expresses the estrogen receptor.

David Raulet and Lewis Lanier, separately, show that tumor cells expressing ligands for the natural killer cell activating receptor NKG2D are destroyed by natural killer cells in vivo, establishing a new mechanism for how natural killer cells recognize tumor cells.

GCSF and hypoxia inducible factor - 1α (HIF - 1α) have been shown to up - regulate SDF - 1α in the infarcted area in a rabbit ischemia model and promote the migration of CXCR4 expressing cells towards cardiac tissues [47 - 49].

In addition, the data showed that the gut's hormone - producing enteroendocrine cells (EECs)-- long divided into subsets based on the idea that each only expressed a single hormone — can actually express multiple hormones at once.

Quantification of HNF4α immunostainings showed that over 90 % of hiPS cell - derived hepatocytes differentiated using our protocol express HNF4α [4].

Albumin and α1AT are well - expressed in enhanced hiPS - HEP cells from all three hiPS cell lines (Figure 3, Panels A, B, and C), as shown by immunostaining and mRNA expression analysis.

However, some reports show that MSC do constitutively express mRNA for cytokines such as IL - 6, -7, -8, -11, -12, -14, -15, -27, leukaemia inhibitory factor, macrophage colony - stimulating factor, and stem cell factor 148, 149.

In cytotoxicity assays employing PPAM, we found no significant differences of IC50 values between SCLC tumor cells, SCLC CTC lines which express the Wnt pathway and normal cells such as HEK293 (data not shown).

Friedrich Miescher Institute scientists Botond Roska and Volker Busskamp have previously shown that some vision can be restored in mice by engineering those cone cells to express light - sensitive proteins.

In our experiments, both HNF4α and ASGPR1 are well - expressed in enhanced hiPS - HEP cells from all three hiPS cell lines (Figure 2), as shown by homogeneous nuclear staining for HNF4α and homogeneous membrane staining for ASGPR1.

Laminin cultured cells arrange themselves in lobule like structures, express MRP1 and MRP2 and are capable of biliary efflux and show significant effect on hepatocyte P450 enzyme metabolic activity.

Here, we show that enhanced hiPS - HEP cells respond to insulin with phosphorylation of protein kinase B - α (Akt), even at low insulin concentrations (Figure 4, Panels E and F), and that the genes involved in glycogen metabolism, gluconeogenesis, and insulin signaling are expressed at similar levels as in hphep cells (Figure 4, Panels B, C, and D).

A, Western blot analysis after subcellular fractionation showed that Karpas 299 and SU - DHL1 cells express phosphorylated mTOR, 4E - BP1, and rpS6 as well as total eIF4E predominantly in the cytoplasm.

Our older data had shown that the molecules B7 and ICAM could costimulate naïve T cell activation when expressed in isolation, but an efficient response, including high levels of CD40L, IL - 2, and short - term proliferation, resulted only when both B7 and ICAM were able to signal through CD28 and LFA - 1 at the same time (Dubey, 1995, J Immunol; Jaiswal, 1996, Int Immunol; Rogers, 1998, J Immunol).

Ilda showed that the Paternally Expressed Gene - 3 (PEG 3) is a novel regulator of somatic cell reprogramming.

FGF2 and its receptors have been shown to be expressed at the protein level by both oocytes and granulosa cells up to secondary follicles [28], but the expression patterns in antral follicles have not been studied to date.

The number and intensity of ROS - stained cells were captured with a GE InCell imager and the percentage of cells expressing ROS above a set threshold were determined; positive values show pairs of means that are significantly different.

Immunohistochemical staining of the automatically monitored colonies showed that these colonies were positive for Nanog and that SSEA1 was expressed in only a small proportion (3 %) of the cells (Figure 2f).

Michael's image shows COS - 7 cells ectopically expressing the Fcγ - receptor performing phagocytosis on beads opsonised with Immunoglobulin G. His false - colour scanning electron micrograph shows filopodia and pseudopodia projecting around the beads, which will subsequently be fully engulfed.