E, ATP (circles) and 5 - HT (triangles) dose — response curves for 5 - HT3AGFP -
expressing geniculate ganglion cells.
Not exact matches
In mice
expressing GFP under the control of the 5 - HT3A promoter, a subset of cells in the
geniculate ganglion and nerve fibers in taste buds are GFP - positive.
We next tested the responsiveness of GFP -
expressing and GFP - nonexpressing
geniculate ganglion neurons to exogenously applied 5 - HT.
Because all taste fibers
express the P2X3 purinergic receptor (Ishida et al., 2009; Vandenbeuch et al., 2015), we immunoreacted
geniculate ganglion sections from 5 - HT3AGFP mice with antibodies against GFP (to amplify 5 - HT3AGFP signal) and against P2X3.
Our data demonstrate that a subset of
geniculate ganglion neurons
expresses 5 - HT3 receptors and innervates taste buds, including serotonergic type III cells.
To first determine whether gustatory afferents
express GFP in 5 - HT3AGFP mice, we examined the
geniculate ganglion and taste buds.