Sentences with phrase «expressing unc»
Neurons expressing unc - 7:: gfp include members of all motor neuron classes (AS9 - 11, DD5, VD10 - 11, VA10 - 11, VB11, DB7, and DA7).
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Lane 1, wild type (N2); lanes 2 and 3, unc - 7 (e5) rescued with unc - 7S:: gfp + cosmid F56B12, expected to express all isoforms; lane 4, unc - 7 (e5) rescued with unc - 7SΔ1S + F56B12, predicted to express UNC - 7L:: and SR:: GFP.
(D) UNC - 7L expressed in interneurons (Punc - 7S:: unc - 7L [M121L]-RRB- rescues forward locomotion in unc - 7 (e5) and localizes to puncta near B motor neuron cell bodies.
Using unc - 9 mutants and heterologous promoters, we showed that expression of UNC - 9 in B class motor neurons is necessary to rescue the localization of UNC - 7S expressed in AVB.
(B) Fully rescuing unc - 7 min plasmid expressing UNC - 7S and UNC - 7SR.
To verify an AVB but not motor neuron requirement for UNC - 7S, UNC - 7S:: GFP under control of the sra - 11 promoter [31] was expressed in unc - 7 (e5).
(A) In wild - type (wt; N2), UNC - 9:: GFP expressed in B motor neurons (Pacr - 5:: unc - 9:: gfp) visualizes puncta in the ventral nerve cord localized near B cell bodies.
(B) unc - 7S:: gfp construct expressed in unc - 7 (e5).
Psra - 11:: unc - 7S:: gfp expressed in AVB did not rescue forward locomotion in e5 animals; the Pcex - 1:: unc - 7s: gfp construct, expressed in AVA and AVD but not AVB, did, however, rescue e5 forward locomotion.
When Psra - 11:: unc - 7S:: gfp was expressed in unc - 9 daf - 6 unc - 7, puncta were not preferentially localized near B motor neurons (Figure 7H), but when co-injected with Pacr - 5:: unc - 9 (B motor neurons) UNC - 7S:: GFP localization was restored (Figure 7I).
In animals rescued with unc - 7SΔ1S + F56B12, predicted to express UNC - 7SR:: GFP but not UNC - 7S:: GFP, only the smaller band was detected.
(B) UNC - 9:: GFP is expressed more uniformly in the ventral nerve cord in unc - 7 (e5) animals, and clusters of puncta near B cell bodies are not discernible.
(E) UNC - 7L expressed in B motor neurons (Pacr - 5:: unc - 7L [M121L]-RRB- in unc - 9 daf - 6 unc - 7 does not localize to puncta but remains associated with cell bodies (red); co-expressed UNC - 9:: GFP (Pacr - 5:: unc - 9:: gfp; green) is distributed throughout the motor neuron processes.
(D) In unc - 9 daf - 6 unc - 7 animals, Pacr - 5:: unc - 9:: gfp is expressed more diffusely in cell bodies (few bright puncta).
When expressed under control of the Punc - 7S promoter, UNC - 7L (Punc - 7S:: unc - 7L [M121L]-RRB- rescued unc - 7 (e5) forward locomotion; though much of the signal remained associated with cell bodies, some puncta were seen localized to AVB: B motor neuron gap junctions (Figure 10D).
Punc - 7S:: unc - 9:: gfp was expressed in unc - 7 (e5) mutants; UNC - 9:: GFP was broadly but weakly expressed as small puncta throughout the ventral nerve cord (Figure 7J), a pattern reminiscent of UNC - 7S expression in unc - 9 unc - 7 double mutants (Figure 7D).
It also suggests that in unc - 9 mutants, the native UNC - 7 isoforms expressed in motor neurons (as detected by previous analysis of total UNC - 7 expression) are insufficient to properly localize AVB - expressed UNC - 7S:: GFP (that is, UNC - 7 in motor neurons does not substitute for UNC - 9 in localizing AVB - expressed UNC - 7S).