Sentences with phrase «expressing wild type»

Not exact matches

Several years ago, while creating a genetically engineered mouse that expresses high levels of the mouse HMGA1 gene to investigate its role in leukemia, Resar and her colleagues made the chance finding that the intestines of these animals were much larger and heavier than those of «wild - type» animals (or control mice that were not genetically modified).

When scientists engineered the plants to express the glutathione transferase (DmGSTE6) gene found in fruit flies, they found that plants expressing the gene were more resistant to TNT and were better able to remove it from contaminated soil than wild - type plants without the gene.

The researchers set out to understand how this drought tolerance came about by comparing the set of expressed genes (the transcriptome) in the mutants to that in normal (so - called wild - type) plants.

Kaplan - Meier analysis demonstrated that most of the mice injected with cells expressing wild - type PREX2 or GFP exhibited tumor - free survival for more than ten weeks.

Injection of wild - type stage 15 embryos with E. coli constitutively expressing Mcf1 from the high - copy vector pUC18 causes rapid paralysis of embryonic hemocytes and inhibition of phagocytosis as observed following wild - type Photorhabdus infection (Figure 3A and Video S3).

Control studies were performed using cells expressing either wild - type PREX2 or green fluorescent protein (GFP).

We regenerated cells stably expressing ectopic wild - type and mutant phosphatidylinositol -3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2) using the same immortalized human NRASG12D melanocytes as the original study.

Remarkably, the relative expression levels of all marker genes were only slightly decreased when R206H ACVR1 and Q207D caACVR1 cultures were cotransfected with Noggin, whereas cells expressing the wild - type ACVR1 or empty viral vector showed clear downregulation of all analyzed marker genes for chondrogenesis.

C. elegans expressing a modified form of human amyloid - β survived three or four more days following infection in the gut with Candida albicans, compared to wild - type worms that did not express the peptide.

Of special note today: gut microbiota species expressing orthologs of human Ro60 might be involved in triggering and sustaining chronic autoimmunity in lupus; The portal vein blood microbiome in patients with liver cirrhosis; A randomized clinical study suggests dietary promotion of short chain fatty acid producing gut microbes as an effective treatment for type 2 diabetes; and the sexual dimorphism of root, flower and leaf microbiomes in the wild strawberry plant

Young mice that expressed high levels of human amyloid - β (but did not have pathological plaques) infected in the brain with Salmonella typhimurium were more likely to survive the infection compared to wild - type mice that did not express the peptide, Tanzi, Moir, and their colleagues found.

(H) and (I) At 15 DAS (H) and 16 DAS (I), SvBd1 was expressed at the boundaries of incipient lateral organs (yellow arrows) in developing spikelets in A10.1 wild - type inflorescences, but not in bristles.

In bsl1 - 1 mutants, Bsl1 signal was comparable to the wild type at early stages of development (Supplemental Figure 9E); however, by ∼ 16 DAS, Bsl1 transcripts became ectopically expressed outside of the defined domain and accumulated at the base of developing spikelets (Figure 7E).

Our RNA - seq analyses showed that SvBd1 (Sevir.2 G437800), encoding the ortholog of a conserved AP2 - ERF TF, which specifies SM identity in maize and other grasses (Chuck et al., 2002), was expressed at substantially higher levels in the bsl1 - 1 mutant compared with the wild type, consistent with increased production of spikelets in place of bristles (Table 2).

(G) At 14 DAS, SvBd1 was expressed in a semicircular domain (yellow arrow) at the base of all AMs prior to spikelet or bristle formation in wild - type inflorescences.

Among the 287 DE genes that were expressed higher in wild - type panicles, functional enrichment analyses showed that genes involved in nutrient sensing and signaling («cellular response to nutrient levels»; GO: 0031669; P = 8.58 e − 04), notably nitrogen and sugar related, were overrepresented.

Given that our wild type B. burgdorferi acquired by xenodiagnostic ticks retain the ability to express ospC (also shown by RT - PCR in Fig 4), we can not rule out the possibility that a tick midgut - adapted phenotype, in the absence of salivation and feeding, contributed to the failure of the B. burgdorferi acquired from XT to infect SCID mice.

Since it is known that GBP regulates growth in a concentration - dependent manner in lepidopterans [16], we used the temperature - sensitive properties of Gal4 to modify the level of transgene expressed in the wild - type background by rearing larvae either at 22 or 25 °C.

Here, we examine wild - type huntingtin's localization in cultured cells by expressing the full - length human protein tagged with enhanced green fluorescent protein (EGFP) within its unspliced genomic context.

(A, B) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells induces low level of ILP2 (A) and ILP5 (B) accumulation in the insulin - producing cells.

(C, D) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells decreases the densities of ILP2 (C) and ILP5 (D) signals in the insulin - producing cells.

I thought I could see GFP expression with a fluorescence microscope, but the GFP expression was so low (even in the transduced wild - type A549 cells that lack Cas9 and thus should have expressed GFP) that I couldn't get a good image.

Lane 1, wild type (N2); lanes 2 and 3, unc - 7 (e5) rescued with unc - 7S:: gfp + cosmid F56B12, expected to express all isoforms; lane 4, unc - 7 (e5) rescued with unc - 7SΔ1S + F56B12, predicted to express UNC - 7L:: and SR:: GFP.

(A) In wild - type (wt; N2), UNC - 9:: GFP expressed in B motor neurons (Pacr - 5:: unc - 9:: gfp) visualizes puncta in the ventral nerve cord localized near B cell bodies.

As a test of this model, that these potential Oct4 phosphorylation events can shape the ES cell transcriptional landscape, Oct4 point mutants in an inducible expression vector can be stably transfected into ES lines which conditionally express wild - type Oct4 [14].

Computational classifiers trained between wild ‐ type cells and cells expressing Cas9 and gRNA enable the profiling of multivariate single cell phenotypes.

As in the genre films such as 1951's Flying Leathernecks that Lucas emulates, the «types» among the Airmen are expressed by nicknames: reckless «Lightning» (David Oyelowo), troubled «Easy» (Nate Parker), impetuous «Ray Gun» (Tristan Wilds), and playful «Joker» (an excellent Elijah Kelley).