The system could be used for analyzing low
expression cell surface proteins and for high - throughput screening for industrial enzyme production.
Not exact matches
Questions the group hopes to answer over the next five years include if LRAs will promote the
expression of viral
protein on the
surface of infected
cells, and if pairing LRAs with immune interventions will lead to the clearance of persistent, latent infection.
In particular, Nguyen and Ehrenstein discovered that adalimumab increases the
expression of TNF on the
surface of patient monocytes and promotes the association of these TNF molecules with receptor
proteins on the
surface of regulatory T
cells.
These findings suggest that animal
cells may have fail - safe mechanisms that prevent the
surface expression of improperly folded
proteins with unpaired or improperly bonded cysteine residues.
F3 can detect the
expression of a
protein called nucleolin, which is a marker on the
surface of tumor
cells.
The scientists also found that a
protein known as CD29 acts as a
cell -
surface marker for precursor fat
cells that can generate mature
cells with high energy potential, as shown by their UCP1
expression.
A
cell -
surface antibody panel was created and used for
protein profiling alongside gene
expression profiling.
The putative merozoite
cell surface and secreted
proteins that were chosen for recombinant
expression
Intestinal permeability was assessed by Ussing chamber; epithelial
cell (EC) ultra-structure by electron microscopy; RNA
expression of genes coding for junctional
proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T -
cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by
surface staining of live bacteria with serum followed by FACS analysis.
The Minnesota team also found that hemangiosarcoma
cells with a high
surface expression of a
protein called colony - stimulating factor 1 receptor (CSF - 1R) were the culprits in this process.