Sentences with phrase «expression in human cells»

Gene constructs were made by gene synthesis (GeneartAG) using sequences that were codon - optimized for expression in human cells.

Dr. Mikhail Liskovykh an expert in Human Artificial Chromosomes (HAC) construction and transfer, including the assembly of centromere arrays, generating conditionally stable HACs, and cloning complete genetic loci for expression in human cells.

Several genetic approaches have been taken to reduce or eliminate CCR5 expression in human cells, including the use of ribozymes [17], [18], single - chain intracellular antibodies [19], trans - dominant coreceptor mutants [20], and RNAi [21], [22].

Not exact matches

Mononuclear phagocytic cells in human milk: HLA - DR and Fc gamma R ligand expression.

This study built on previous research from the Sundrud lab, which showed that when TH17 cells entered the intestine in human tissue samples, they increased the expression of a gene called MDR1.

RNAScope ISH was developed by Advanced Cell Diagnostics (ACD) Inc., initially for studies of gene expression in animal (and especially human) tissues.

The researchers have compared various processes involved in gene expression, such as gene transcription and chromatin modification, and have repeated this in different tissues and cell types from both humans and mice.

Bloch's colleagues at the National Institute of Environmental Health Sciences tested the oils in gene expression studies on lab - grown human breast cancer cells and found that they could mimic estrogens, the primary female sex hormones, and inhibit androgens, the primary male sex hormones.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

In a paper publishing August 7th in the Open Access journal PLOS Biology, researchers at the Max Planck Institute of Molecular Cell Biology and Genetics (MPI - CBG) succeeded in mimicking the sustained expression of the transcription factor Pax6 as seen in the developing human brain, in mouse cortical progenitor cellIn a paper publishing August 7th in the Open Access journal PLOS Biology, researchers at the Max Planck Institute of Molecular Cell Biology and Genetics (MPI - CBG) succeeded in mimicking the sustained expression of the transcription factor Pax6 as seen in the developing human brain, in mouse cortical progenitor cellin the Open Access journal PLOS Biology, researchers at the Max Planck Institute of Molecular Cell Biology and Genetics (MPI - CBG) succeeded in mimicking the sustained expression of the transcription factor Pax6 as seen in the developing human brain, in mouse cortical progenitor cellin mimicking the sustained expression of the transcription factor Pax6 as seen in the developing human brain, in mouse cortical progenitor cellin the developing human brain, in mouse cortical progenitor cellin mouse cortical progenitor cells.

Support for this scenario was unexpectedly found in humans, where expression of Crhbp and Pmch are extremely closely linked in the hypothalamus, indicating they may actually be present in the same cells and together act downstream of Lef1 to regulate behavior.

SIX3 and a related gene, SIX2, with a similar pattern of expression in human beta cells, encode proteins known as transcription factors that control the expression of many other genes in the cell.

The results showed that, when applied, a long - term inhibition of Rho - kinase signaling increased the expressions of chondrocyte - specific genes and differentiation markers in human chondrosarcoma 2/8 cells.

They also applied it to human brain tissue collected by the Genotype - Tissue Expression (GTEx) Project, finding that they could a) identify expression signatures unique to neurons, glial cells, and other cell types in the brain (including rare types), and b) differentiate between closely related cellExpression (GTEx) Project, finding that they could a) identify expression signatures unique to neurons, glial cells, and other cell types in the brain (including rare types), and b) differentiate between closely related cellexpression signatures unique to neurons, glial cells, and other cell types in the brain (including rare types), and b) differentiate between closely related cell subtypes.

«This is a unique and valuable resource for researchers wishing to begin to understand how gene expression is dynamically regulated in human islet cells,» said Kim.

They then examined genetic variants throughout the human genome for their effects on gene expression in these two representative populations of immune cells.

Second, it helps cells that produce Hedgehog (Sonic hedgehog in humans) to survive and proliferate in order to induce the expression of Dpp (BMP in humans), a molecule that organises the patterning and growth of the whole wing.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

To corroborate the findings, the researchers also developed a novel mouse model that was deficient for autophagy specifically in beta cells with expression of the human form of islet amyloid polypeptide.

They confirmed low levels of miR - 184 expression in human glioma tissue samples and cultured cell lines as well as an increase in the expression of SND1 compared to normal brain tissue.

The team used gene expression profiling and found that canine B - cell lymphoma expression profiles were similar in many ways to human B - cell lymphoma, thus paving the way for future studies, including therapeutic clinical trials in dogs and humans.

In preclinical experiments using human prostate cancer cell lines, Fu's team showed that increased PLK1 expression activated an oncogene known as c - RAF, which has previously been shown to play a role in regulating cell growth and divisioIn preclinical experiments using human prostate cancer cell lines, Fu's team showed that increased PLK1 expression activated an oncogene known as c - RAF, which has previously been shown to play a role in regulating cell growth and divisioin regulating cell growth and division.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in micIn the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in micin breast cancer - derived cell lines and in human breast cancers replicated in micin human breast cancers replicated in micin mice.

Using single cell RNA sequencing analysis, the Cairns lab profiled cells individually, establishing the gene expression profile in human sperm stem cells.

In a Cell paper published on April 7, Lanner's team analysed gene expression in 88 early human embryos and is using those data to identify genes to disrupt in embryos using CRISPR — CasIn a Cell paper published on April 7, Lanner's team analysed gene expression in 88 early human embryos and is using those data to identify genes to disrupt in embryos using CRISPR — Casin 88 early human embryos and is using those data to identify genes to disrupt in embryos using CRISPR — Casin embryos using CRISPR — Cas9.

«Because the primary Small Intestine Chip recapitulates the physical microenvironment that cells experience inside the human body, such as fluid flow and cyclic peristalsis - like stretching motions, it exhibits a genome - wide gene expression profile that comes closer to its in vivo counterpart than that of the same intestinal cells grown as 3D organoids,» said first - author Magdalena Kasendra, Ph.D., a former Postdoctoral Fellow on Ingber's team and now Principal Scientist at Emulate, Inc. in Boston.

Every cell contains the complete set of DNA in the human genome, but most of it is tightly spooled away in storage and unavailable for expression.

This image shows an expression of the stem cell gene Musashi in human pancreatic cancer.

«We were excited to see that in human liver tumors mTORC1 signaling correlates with FGF21 expression,» comments cell biologist Dr. Marion Cornu and first author of the study.

In tests using human neural progenitor cells (NPCs)-- self - renewing, multipotent cells that generate neurons and other brain cell types — the scientists found that exposure to sofosbuvir not only rescued dying NPCs infected with the Zika virus, but restored gene expression linked to their antiviral response.

In four cell lines derived from human and monkey, siRNAs substantially lowered the expression of three of four test genes — by as much as 90 %.

«Within 3 weeks after expression of the NeuroD1 protein, we saw in the microscope that human glial cells were reinventing themselves: they changed their shape from flat sheet - like glial cells into normal - looking neurons with axon and dendritic branches,» Chen said.

Cell cycle protein expression and proliferative status in human corneal cells.

Human transforming growth factor - beta complementary DNA sequence and expression in normal and transformed cells.

AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting of the neuron - specific class III b - tubulin isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».

In mice containing transplanted human cells carrying about 310 GAA repeats, the prosthesis restored expression of a signaling protein to nearly normal.

By suppressing genes that are active in the developing embryo, silenced just before birth, and re-activated years later in many advanced cancers, the let - 7 family of «microRNAs» — tiny snippets of RNA that can put the brakes on expression of selected genes — appears to prevent human cancer cells from reasserting their prenatal capacity to divide rapidly, travel and spread.

Expression of cell cycle - associated proteins in human and rabbit corneal endothelium in situ.

«This data allows classification of all human protein - coding genes into those coding for house - hold functions (present in all cells) and those that are tissue - specific genes with highly specialized expression in particular organs and tissues, such as kidney, liver, brain, heart, pancreas.

Noggin strongly induces the expression of the EFTFs in frog primitive ectoderm and human ES cells [3], [7].

Paull AC, Whikehart DR. Expression of the p53 family of proteins in central and peripheral human corneal endothelial cells.

CEBPG regulates ERCC5 / XPG expression in human bronchial epithelial cells and this regulation is modified by E2F1 / YY1 interactions.

Our technological expertise ranges from the most fundamental approaches to study membrane transport in lymphocytes and dendritic cells (subcellular compartmentalization, intravital microscopy, phagosomal functions), the systematic analysis of gene expression and it regulation (RNAseq, Chip Seq, proteomics) and physiological and pathological immune responses (mouse models for cancer immunity, immunomodulation / vaccination, human clinical studies in cancer).

Expression of functional Toll - like receptors 2 and 4 in human aortic valve interstitial cells: potential roles in aortic valve inflammation and stenosis.

Highly Synchronized Expression of Lineage - Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines.

Induction of stem cell gene expression in adult human fibroblasts without transgenes.

His lab is interested in the regulation of gene expression by mRNA processing in C. elegans and human cells.

Although persistent loss of IGF - 1R expression ultimately induced cell stasis and death, both of these processes are regulated by the tumor suppressor gene p53 that is commonly mutated in human prostate cancers.

High mRNA expression levels of HNF4α and ASGPR1 are shown in the enhanced hiPS - HEP cells for an extended culture time and are comparable to levels in cryopreserved human primary hepatocytes (hphep cells) that have been grown for only a short time.

Decreased functional expression of Grp78 and Grp94 inhibits proliferation and attenuates apoptosis in a human gastric cancer cell line in vitro.