Sentences with phrase «expression levels using»

Identifying adaptive and plastic gene expression levels using a unified model for expression variance between and within species.

Identifying adaptive and plastic gene expression levels using a unified model for expression variance between and within species Rohlfs, R. and R. Nielsen.

Not exact matches

That site has some really excellent posters you might want to take a look at as well (oh, and it might help get the extra layer of meaning if you know that «po - mo» is also slang for «post modern» which is a term used to describe the meta - level / self - satirize / surreal sort of cultural expression that followed the «modernist» movements): http://www.spurgeon.org/~phil/posters.htm

That goal is the expression, on the level of duty, of the demand, the claim — the Verlangen — which constitutes pure reason in its speculative and practical use; reason «demands the absolute totality of conditions for a given conditioned thing» (beginning of the Dialectic of the Critique of Practical Reason).

Because the deeper, nonverbal levels tend to be impoverished in our culture, worship should concentrate special attention on these levels through the use of symbolic and artistic expressions.

Such an idea contradicts the constant practice of the Church in which subsequent teachings of Popes, Councils and the Catechism reiterate, sometimes using different words and expressions, prior non-infallible teachings of Popes (in encyclicals) and other documents to be accepted and authoritatively binding at the appropriate level.

The pump automatically starts at expression mode and if your last suction level was 10, the next time you use it will start from 10 which can be a major shocker.

When using the expression phase, the highest vacuum level is 12 and the highest cycle is 54.

DNA methylation is a mechanism cells use to control gene expression at the epigenetic level.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

They also showed that they could manipulate Klotho expression pharmacologically using the anti-diabetic drug rosiglitazone, which resulted in decreased levels of Wnt5A.

The tool then uses information from similar cells to make an educated guess about what the level of gene expression should be.

«The genes which these miRNAs regulate also had increased levels, indicating that these gene expression, indicating that these gene products were likely targeted for storage and for possible future use within the brain cell, rather than for destruction.

In the study published in Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signals.

Using data from a large public brain tumor database called REMBRANDT, the researchers confirmed that patients with lower levels of SND1 survived longer than those with elevated SND1 expression.

Then, using Mayo Clinic's large panel of human pancreatic cancer biopsies, the scientists found that expression levels of Rac1b were significantly associated with the cancer's prognosis.

Natural variation exists in the levels of pectate lyase gene expression in wild relatives of cultivated tomato and these can be used for conventional breeding purposes.

Scharff and her colleagues used RNA interference (RNAi), a procedure that inhibits gene expression, to reduce the levels of FOXP2 in zebra finches.

Using this approach, these investigators found that nodes (genes) with high connectivity tend to have lower levels of change in expression than peripheral nodes, consistent with the notion that disease ‐ causing genes are typically not central hubs in a molecular module.

This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 + endothelial cells, and CD45 + immune cells isolated from lungs of mice with macrometastases, which is a replication of the experiment reported in Figure 2H.

The addition of IFN - γ was used to enhance the expression of the MHC H - 2Kb class I promoter, which regulates the level of large T antigen protein in ImmortoMouse - derived cells (10).

Wilcoxon rank - sum test was used to calculate the P value for ex vivo bioluminescence from brain and expression level of miR - 7 and KLF4 in human specimens.

To identify transcriptional patterns that correlate with body mass, we used oligonucleotide microarrays to catalogue gene expression levels in the parametrial or epididymal adipose tissue from two dozen mice whose body mass and adiposity varied due to diet, sex, and mutations in genes affecting energy homeostasis.

We used Kendall's τ statistic, a rank - based test of correlation, to detect transcripts whose expression levels were significantly correlated with body mass.

Using quantitative RT - PCR we determined the relative expression levels of the macrophage expressed gene Cd68 in human abdominal subcutaneous adipose tissue.

Gene expression patterns in the hippocampus samples were examined using DNA microarrays that measured the expression levels of over 30,000 genes (transcripts) per sample.

When mapped to the genome, they tell us not only about the level of expression for various genes, but also which transcription start sites were used.

(Mixture - of - Isoforms) for isoform quantitation using RNA - Seq is a probabilistic framework that quantitates the expression level of alternatively spliced genes from RNA - Seq data, and identifies differentially regulated isoforms or exons across samples.

On the contrary, quantitative RT - PCR using the RNAs extracted from the MBs revealed that the difference in the Acks expression level in the MBs between the antennae - deprived and intact workers under high temperature was not statistically significant.

To determine expression levels, in traditional sequencing methods a mix of millions of cells is used.

We induced seizure by awakening the Japanese honeybee workers from CO2 - induced anesthesia at room temperature (25 °C) as described previously [13], and measured the Acks expression levels in the whole brain using quantitative RT - PCR.

cDNA synthesized by reverse transcription of 1 µg RNA primed with oligo (dT) and random 9 - mer primers was used as the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in the presence of SYBR ® Green (Stratagene, La Jolla, CA).

The expression level of CXCR4 can be used as an independent prognostic factor for disease free survival of SCLC patients [37].

Expression levels of Bsl1 and SvDwf4 were analyzed by a standard curve method using equal amounts of total RNA per sample.

Protein expression levels were quantitated using the software Scion Image (Scion Corporation, Frederick, MD).

In the 1980s, Dr. Kaufman's experience with gene transfer and engineering led him to become a founding scientist at Genetics Institute Inc., where he engineered mammalian cells for high - level expression of therapeutic proteins, such as clotting factors that are now used to treat individuals with hemophilia.

Lucas Pelkmans and colleagues use much brighter probes, allowing them to perform rapid and robust low - magnification imaging of many more cells, quantify low - level expression accurately and also query very short RNA transcripts.

We used CIBERSORT35, to deconvolute bulk gene expression into expression levels for 18 different cell types.

High level erythroid lineage - directed gene expression using globin gene regulatory elements following lentiviral vector mediated gene transfer into primitive human and murine hematopoietic cells.

This can then be used to create computational estimates of gene expression levels from any whole genome sequence or chip dataset.

«Methods currently used for measuring gene expression generally rely on something called «relative normalisation», which means that gene expression levels in a sample can only be estimated relative to other genes in the same sample.

Dr Gilchrist said: «This study suggests that we may improve significantly on the widely used analysis methods for determining gene expression levels from high throughput sequence data: absolute quantitation offers a much sounder basis for determining changes in gene expression level, a measure widely used to determine the consequence of genetic, chemical or physical disturbances in living systems.»

The overall score encompass several factors: reproducibility of the antibody staining in different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.

Using both semi-quantitative-reverse transcriptase PCR and Quantitative Real - Time PCR to assess the resulting levels of sema2a expression, we confirmed that our method of RNAi significantly decreased sema2a levels (Figure 1A - B).

Average fold change and SD of expression levels is shown for three independent experiments using 9W - 1 hGPCs.

Another widely used technology to profile gene expression levels is microarrays.

Additionally, using RNA - Seq, we determined changes in gene expression levels between the wild - type (WT) and DAXX knock - down (K / D) PC3 cells.

Pairwise comparisons were performed using standard statistical conditions (FDR < 0.05, > 1.5-fold expression levels) to unveil genes up - regulated or down - regulated between the populations.

For example, by using histone deacetylase (HDAC) inhibitors, which, by remodeling chromatin structure, are thought to promote gene expression broadly, including for SMN2, numerous groups showed that they could increase SMN protein levels — both truncated and full - length — in mouse models of SMA as well as in patient - derived cell lines.

Using this cell line we show that apparently undifferentiated ES cell cultures consist of at least three cell types defined by this lineage - specific low - level transcription and the expression of the ES cell markers Oct4 and Nanog.