Sentences with phrase «expression of a single gene»

In 1993 she proved that turning down the expression of a single gene, daf - 2, through genetic engineering doubled the worm's life span.

That is easy to explain by a feature that we now recognize as universal: the plasticity of expression of single genes.

«To our knowledge, our model is the first in which expression of a single gene in postnatal hair cells results in hair cell survival and hearing preservation in mice that otherwise suffer from age - related and noise - induced hearing loss,» Dr. Chen said.

Promiscuous male voles can be turned into paragons of monogamous virtue simply by increasing the expression of a single gene.

Thus I was able to ask how the expression of this single gene limits the size of the haltere.

Not exact matches

A single miRNA can ramp down expression of multiple genes — but the number of genes affected by known miRNAs hasn't been determined.

Scientists have shifted from studying single molecules to investigating large complexes of interacting biological macromolecules involved in processes such as metabolic pathways, gene expression, and development of disease.

The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be used in any type of single - cell gene - expression analysis.

In a paper published in Nature Methods, postdoctoral fellows Naomi Habib, Inbal Avraham - Davidi, and Anindita Basu; core institute members Feng Zhang and Aviv Regev; and their colleagues reveal DroNc - Seq, a single - cell expression profiling technique that merges sNuc - Seq with microfluidics, allowing massively parallel measurement of gene expression in structurally - complicated tissues.

The researchers found that the blond hair commonly seen in Northern Europeans is caused by a single change in the DNA that regulates the expression of a gene that encodes a protein called KITLG, also known as stem cell factor.

Traditionally, gene function was determined one gene at a time, but today's microarray technology enables scientists to monitor the expression of tens of thousands of genes on a single glass slide.

For Helicobacter pylori, a pathogenic bacterium that colonizes over 40 percent of the world population and is associated with gastric cancer, the team discovered that epigenetic heterogeneity can quickly emerge as a single cell divides, and different subpopulations with distinct methylation patterns have distinct gene expressions patterns.

Once again, they saw that methylation of a single spot in the serotonin transporter gene was associated with lower levels of serotonin transporter expression in the amygdala.

When a single gene exists in the ancestral mutation, that would have made for higher levels of enzyme expression, would have messed up with sensory function.

Single - cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the TEMRA (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4 - CTLs, compared with CD4 + T cells in the central memory (TCM) and effector memory (TEM) subsets.

The genomics era has had a profound impact on life science research, leading to significant developments such as the use of expression quantitative trait loci (eQTLs), which link polymorphisms in single genes to quantifiable changes in gene expression associated with specific diseases.

Gene moonlighting can occur merely through changes in expression, which may result from as little as a single mutation; it does not require the meandering process of random alteration and selection implied by the duplication and neofunctionalization model.

At the cellular level, although they share canonical types of inhibitory interneurons (INs) and excitatory principal neurons (PNs), it remains largely unknown to what extent a single type in different brain regions displays similarity in gene expression, axonal shape, connectivity, and developmental origins.

In their new Science paper, the researchers analyzed gene expression in single cells across key developmental time points and from different regions of the brain.

The team singled out the gene Mecp2, which encodes a protein that regulates the expression of genes that help forge connections between nerve cells.

Single - stranded, noncoding micro-ribonucleic acids (microRNAs), consisting of 18 - 23 nucleotides, play a key role in regulating gene expression.

This single cell transcriptome analysis followed by computational analyses enabled the team to identify the gene expression profiles of cells in the process of changing from ES cells to 2CLCs.

The researchers then developed a new type of single - strand RNA interference (RNAi) agent, the proline - modified short hairpin RNA (PshRNA), which suppresses gene expression by utilizing the cellular system.

This has led to the analysis of single cell gene expression - ie which genes are switched on in a cell.

Topics for the scientific sessions are: Single cell genomics and gene expression Genetic interactions RNAi and somatic cell genetics Protein - DNA interactions Cancer The meeting also highlights existing opportunities for academic and industrial researchers to access automated cell - based and biochemical technologies based at the Karolinska High Throughput Center; home to one of the most sophisticated, state - of - the - art, ultra-high performance liquid handling and analysis platforms in Europe.

Bethesda, USA (2016 - present) Research areas: Super-resolution microscopy, single - molecule imaging, gene expression, computational modeling and data analysis This section includes all projects during my postdoctoral research stay at the National Institutes of Health in Bethesda, MD (Unites States): (9) Understanding gene expression in eukaryotic cells»

The gene expression from every sample was amplified in triplicates, with a single volume of 10 µl containing: 3 µl of diluted cNDA with 0.5 µl of primer set (see Table 1 for primer sequences), 1.5 µl double distilled water (DDW) and 5 µl of GoTaq qpcr Master Mix Kit (Promega, USA).

The method, PrediXcan, estimates gene expression levels across the whole genome — a better measure of biological action than single mutations — and integrates it with genome - wide association study (GWAS) data.

Conditional expression of hairpin constructs in Drosophila is a powerful method to disrupt the activity of single genes with a spatial and temporal resolution that is impossible, or exceedingly difficult, using classical genetic methods.

Comparison of mRNA and protein expression revealed distinct expression profiles for many genes and underscores the importance of multi-omic analysis in single cells.

For the 374 expressed genes, we find many regions with statistically significant association of single nucleotide polymorphisms (SNPs) with expression variation in lymphoblastoid cell lines after correcting for multiple tests.

July 3, 2013 New mechanism for human gene expression discovered In a study that could change the way scientists view the process of protein production in humans, University of Chicago researchers have found a single gene that encodes two separate proteins from the same sequence of messenger RNA.

Our siRNA molecules are designed to target a single mRNA in the cell, only affecting the expression of the disease - causing gene.

The main research focus of the single molecule group is the study of molecular motors, the role of force in regulating biological systems, and the molecular mechanisms of gene expression regulation.

Research Focus: I dedicate my time and effort to develop high - throughput sequencing technologies such as single cell transcriptomic, ChIP - Seq, ChIA - PET, and many more... The scientific rational is to understand better and with a different angle, the mechanisms of epigenetic regulation of gene expression in (rare) immune cells, pathologically relevant in many diseases such as asthma, SLE, tuberculosis...

microRNAs (miRNA)-- small, hairpin - like structures of single - stranded RNA molecules that can regulate the expression of protein - coding genes.

Single loci with major effects in fire ants: the effects of the complementary sex - determining locus and the green beard supergene on gene expression

To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells.

Developing novel tools to quantify gene expression in single cells As it has become increasingly apparent that gene expression in individual cells deviates significantly from the average behavior of cell populations, new methods that provide accurate integer counts of mRNA copy numbers in individual cells are needed.

The Kind group studies the regulation of gene expression in single cells by developing and using novel microscopy and genomics based techniques.

His group work on understanding the regulation of gene expression, especially at the single - cell level where he has pioneered analytical approaches.

Home > Press > Single - cell mRNA cytometry via sequence - specific nanoparticle clustering and trapping: Cell - to - cell variation in gene expression creates a need for techniques that can characterize expression at the level of individual cells

To uncover molecular processes in individual cells and to understand the full complexity of biological systems, our lab applies and develops novel microscopy and genomics based techniques to study the regulation of gene - expression in single cells.

(A) Expression of 5 stem marker genes in single cells isolated from the edge (E), mid (M) or adjacent center (C) region of HES2 colonies.

These findings on gene expression in single embryonic stem cells are in concert with recent studies of early mammalian development, which reveal molecular heterogeneity and a stochasticity of gene expression in blastomeres.

Methods: Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons.

Random fluctuations in transcription are unavoidable and a general aspect of life at the single - cell level and lead to «noise» around the average level of gene expression.

Expression of four stem cell marker genes normalized to cyclophilin in nine single cell equivalents prepared from a pool of nine lysed HES3 cells.

We successfully established sequencing - based protocols to quantify gene expression of thousands of single cells.

The present study shows that such a gradient is also seen at the level of single cell analysis, but reveals that there is considerable cell - to - cell variation within this gradient in the expression of pluripotency genes.