A. Fractionation of HES 3 or H9 cells by flow cytometry according to the levels of
expression of cell surface markers (GCTM - 2, pericellular matrix proteoglycan, and CD9).
We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize
the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).
Not exact matches
Researchers from BUSM and the University
of Cyprus compared the
markers on the
surface of the cancer
cells to gene
expression profile
of breast tumors deposited by researchers in international public databases and found that a molecule named IL13RA2 (IL13R alpha2) was abundant in metastatic or late - stage BLBC.
The use
of cell surface markers to isolate specific
cell populations is one common method for separating
cells; however, isolating live
cells based on their RNA
expression is a powerful new way enabling the study
of small
cell niches in nongenetically modified animal models and human tissue.
F3 can detect the
expression of a protein called nucleolin, which is a
marker on the
surface of tumor
cells.
We will develop mathematical models to predict the pathways
of differentiation from naive to memory and effector T -
cell subsets based on the characterisation
of surface marker expression, transcription factors and cytokines production at early and late time points after immunisation.
To compare T
cell phenotypes between DGKζ − / − and Cbl - b − / − mice, and to evaluate DKO mice, we isolated thymi, lymph nodes, and spleens from mice
of each genotype and determined
expression of T
cell surface markers.
Differential
expression of surface markers in mouse bone marrow mesenchymal stromal
cell subpopulations with distinct lineage commitment.
Lineage - restricted progenitors are identifiable by
expression of distinct combinations
of cell surface markers.
ES
cells can be described based on a characteristic morphology, the presence
of cell surface markers such as SSEA - 1 and Pecam1, or the
expression of the key transcription factors such as Oct4, Sox2, Nanog, and a number
of ES
cell - specific transcripts (ECATs)[4]--[6].
In the present study, we again observed a continuous gradient in the
expression of pluripotency genes across the
cell populations that paralleled the gradient in
cell surface marker expression.
(B) Percent
of sorted single HES3
cells in (A) expressing stem or lineage
markers according to level
of surface marker expression.
Our laboratory used two monoclonal antibodies, GCTM - 2 and TG30, recognizing the
cell surface proteoglycan characteristic
of primate ES
cells and CD9 respectively, to fractionate human ES
cell populations into four compartments according to their relative levels
of expression of both
markers [14].
Cells along the continuum show a progressively decreasing likelihood
of self renewal as their
expression of stem
cell surface markers and pluripotency genes wanes.
Single
cell Q - RTPCR for the genes indicated was carried out on
cells separated by flow cytometry on the basis
of cell surface marker expression.
Oct - 4 is most consistently expressed
of the pluripotency genes that we have studied, and it is switched off only in populations that have lost other measurable features
of pluripotency, such as stem
cell surface marker expression and the capacity for self - renewal.