That is easy to explain by a feature that we now recognize as universal: the plasticity of
expression of single genes.
In 1993 she proved that turning down
the expression of a single gene, daf - 2, through genetic engineering doubled the worm's life span.
«To our knowledge, our model is the first in which
expression of a single gene in postnatal hair cells results in hair cell survival and hearing preservation in mice that otherwise suffer from age - related and noise - induced hearing loss,» Dr. Chen said.
Promiscuous male voles can be turned into paragons of monogamous virtue simply by increasing
the expression of a single gene.
Thus I was able to ask how
the expression of this single gene limits the size of the haltere.
Not exact matches
A
single miRNA can ramp down
expression of multiple
genes — but the number
of genes affected by known miRNAs hasn't been determined.
Scientists have shifted from studying
single molecules to investigating large complexes
of interacting biological macromolecules involved in processes such as metabolic pathways,
gene expression, and development
of disease.
The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be used in any type
of single - cell
gene -
expression analysis.
In a paper published in Nature Methods, postdoctoral fellows Naomi Habib, Inbal Avraham - Davidi, and Anindita Basu; core institute members Feng Zhang and Aviv Regev; and their colleagues reveal DroNc - Seq, a
single - cell
expression profiling technique that merges sNuc - Seq with microfluidics, allowing massively parallel measurement
of gene expression in structurally - complicated tissues.
The researchers found that the blond hair commonly seen in Northern Europeans is caused by a
single change in the DNA that regulates the
expression of a
gene that encodes a protein called KITLG, also known as stem cell factor.
Traditionally,
gene function was determined one
gene at a time, but today's microarray technology enables scientists to monitor the
expression of tens
of thousands
of genes on a
single glass slide.
For Helicobacter pylori, a pathogenic bacterium that colonizes over 40 percent
of the world population and is associated with gastric cancer, the team discovered that epigenetic heterogeneity can quickly emerge as a
single cell divides, and different subpopulations with distinct methylation patterns have distinct
gene expressions patterns.
Once again, they saw that methylation
of a
single spot in the serotonin transporter
gene was associated with lower levels
of serotonin transporter
expression in the amygdala.
When a
single gene exists in the ancestral mutation, that would have made for higher levels
of enzyme
expression, would have messed up with sensory function.
Single - cell differential
gene expression analysis revealed a spectrum
of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the TEMRA (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4 - CTLs, compared with CD4 + T cells in the central memory (TCM) and effector memory (TEM) subsets.
The genomics era has had a profound impact on life science research, leading to significant developments such as the use
of expression quantitative trait loci (eQTLs), which link polymorphisms in
single genes to quantifiable changes in
gene expression associated with specific diseases.
Gene moonlighting can occur merely through changes in
expression, which may result from as little as a
single mutation; it does not require the meandering process
of random alteration and selection implied by the duplication and neofunctionalization model.
At the cellular level, although they share canonical types
of inhibitory interneurons (INs) and excitatory principal neurons (PNs), it remains largely unknown to what extent a
single type in different brain regions displays similarity in
gene expression, axonal shape, connectivity, and developmental origins.
In their new Science paper, the researchers analyzed
gene expression in
single cells across key developmental time points and from different regions
of the brain.
The team
singled out the
gene Mecp2, which encodes a protein that regulates the
expression of genes that help forge connections between nerve cells.
Single - stranded, noncoding micro-ribonucleic acids (microRNAs), consisting
of 18 - 23 nucleotides, play a key role in regulating
gene expression.
This
single cell transcriptome analysis followed by computational analyses enabled the team to identify the
gene expression profiles
of cells in the process
of changing from ES cells to 2CLCs.
The researchers then developed a new type
of single - strand RNA interference (RNAi) agent, the proline - modified short hairpin RNA (PshRNA), which suppresses
gene expression by utilizing the cellular system.
This has led to the analysis
of single cell
gene expression - ie which
genes are switched on in a cell.
Topics for the scientific sessions are:
Single cell genomics and
gene expression Genetic interactions RNAi and somatic cell genetics Protein - DNA interactions Cancer The meeting also highlights existing opportunities for academic and industrial researchers to access automated cell - based and biochemical technologies based at the Karolinska High Throughput Center; home to one
of the most sophisticated, state -
of - the - art, ultra-high performance liquid handling and analysis platforms in Europe.
Bethesda, USA (2016 - present) Research areas: Super-resolution microscopy,
single - molecule imaging,
gene expression, computational modeling and data analysis This section includes all projects during my postdoctoral research stay at the National Institutes
of Health in Bethesda, MD (Unites States): (9) Understanding
gene expression in eukaryotic cells»
The
gene expression from every sample was amplified in triplicates, with a
single volume
of 10 µl containing: 3 µl
of diluted cNDA with 0.5 µl
of primer set (see Table 1 for primer sequences), 1.5 µl double distilled water (DDW) and 5 µl
of GoTaq qpcr Master Mix Kit (Promega, USA).
The method, PrediXcan, estimates
gene expression levels across the whole genome — a better measure
of biological action than
single mutations — and integrates it with genome - wide association study (GWAS) data.
Conditional
expression of hairpin constructs in Drosophila is a powerful method to disrupt the activity
of single genes with a spatial and temporal resolution that is impossible, or exceedingly difficult, using classical genetic methods.
Comparison
of mRNA and protein
expression revealed distinct
expression profiles for many
genes and underscores the importance
of multi-omic analysis in
single cells.
For the 374 expressed
genes, we find many regions with statistically significant association
of single nucleotide polymorphisms (SNPs) with
expression variation in lymphoblastoid cell lines after correcting for multiple tests.
July 3, 2013 New mechanism for human
gene expression discovered In a study that could change the way scientists view the process
of protein production in humans, University
of Chicago researchers have found a
single gene that encodes two separate proteins from the same sequence
of messenger RNA.
Our siRNA molecules are designed to target a
single mRNA in the cell, only affecting the
expression of the disease - causing
gene.
The main research focus
of the
single molecule group is the study
of molecular motors, the role
of force in regulating biological systems, and the molecular mechanisms
of gene expression regulation.
Research Focus: I dedicate my time and effort to develop high - throughput sequencing technologies such as
single cell transcriptomic, ChIP - Seq, ChIA - PET, and many more... The scientific rational is to understand better and with a different angle, the mechanisms
of epigenetic regulation
of gene expression in (rare) immune cells, pathologically relevant in many diseases such as asthma, SLE, tuberculosis...
microRNAs (miRNA)-- small, hairpin - like structures
of single - stranded RNA molecules that can regulate the
expression of protein - coding
genes.
Single loci with major effects in fire ants: the effects
of the complementary sex - determining locus and the green beard supergene on
gene expression
To directly measure the level
of coordination in the
expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs
of functionally related and unrelated
genes within
single Saccharomyces cerevisiae cells.
Developing novel tools to quantify
gene expression in
single cells As it has become increasingly apparent that
gene expression in individual cells deviates significantly from the average behavior
of cell populations, new methods that provide accurate integer counts
of mRNA copy numbers in individual cells are needed.
The Kind group studies the regulation
of gene expression in
single cells by developing and using novel microscopy and genomics based techniques.
His group work on understanding the regulation
of gene expression, especially at the
single - cell level where he has pioneered analytical approaches.
Home > Press >
Single - cell mRNA cytometry via sequence - specific nanoparticle clustering and trapping: Cell - to - cell variation in
gene expression creates a need for techniques that can characterize
expression at the level
of individual cells
To uncover molecular processes in individual cells and to understand the full complexity
of biological systems, our lab applies and develops novel microscopy and genomics based techniques to study the regulation
of gene -
expression in
single cells.
(A)
Expression of 5 stem marker
genes in
single cells isolated from the edge (E), mid (M) or adjacent center (C) region
of HES2 colonies.
These findings on
gene expression in
single embryonic stem cells are in concert with recent studies
of early mammalian development, which reveal molecular heterogeneity and a stochasticity
of gene expression in blastomeres.
Methods: Using
single cell RNA sequencing, we have generated an atlas
of gene expression in murine POMC neurons.
Random fluctuations in transcription are unavoidable and a general aspect
of life at the
single - cell level and lead to «noise» around the average level
of gene expression.
Expression of four stem cell marker
genes normalized to cyclophilin in nine
single cell equivalents prepared from a pool
of nine lysed HES3 cells.
We successfully established sequencing - based protocols to quantify
gene expression of thousands
of single cells.
The present study shows that such a gradient is also seen at the level
of single cell analysis, but reveals that there is considerable cell - to - cell variation within this gradient in the
expression of pluripotency
genes.