Sentences with phrase «expression of the reporter»

Both experiments showed that the wild - type allele had greater DNA - binding capacity, and drove higher expression of the reporter gene.

It was noteworthy that IRF - 1 alone could significantly induce the expression of the reporter gene containing RIG - G promoter (Fig. 1C).

Not exact matches

Legal actions launched by the government targeting reporters to demand the names of confidential sources represent gravest threat to journalistic expression in Canada, said a senior official with Reporters Without Borders amid celebrations of World Press Freedom Day reporters to demand the names of confidential sources represent gravest threat to journalistic expression in Canada, said a senior official with Reporters Without Borders amid celebrations of World Press Freedom Day Reporters Without Borders amid celebrations of World Press Freedom Day on May 3.

Moreover, new Hungarian media laws adopted in late 2010 have been widely criticised, including by the Media Freedom Representative of the Organisation for Security and Co-operation in Europe (OSCE), the International Press Institute, Reporters Without Borders, the UN Special Rapporteur on the Promotion and Protection of the Right to Freedom of Opinion and Expression and by major international newspapers.

The only contrary expression (at least as far as a non-Spanish speaking reporter could discern) was a small group of men near 175th Street chanting «Trump, Trump, Trump.»

When a target gene is unmethylated, the reporter is also unmethylated, triggering expression of a glowing protein that illuminates the cell.

As shown in Fig. 2D, the reporter activity of 3 ′ UTR of KLF4 was significantly suppressed by miR - 7, whereas cotransfection of LNA targeting miR - 7 stimulated the expression of this gene.

Ten nanograms of reporter plasmid and 200 ng of miR7 expression plasmid were cotransfected with 1 ng of phRG - TK Renilla luciferase internal control plasmid (Promega) into 293TN cells using Lipofectamine 2000 reagent (Invitrogen).

We then infected these cells with two retrovirus encoding SOX2 and Ascl1, and you will see sometimes the images depicting the fluorescence, the reporter of fluorescence indicating the expression of the two transcription factors.

Together, these reporter gene experiments confirmed the expression of Tie2 by pericytes and further validated Ng2 - Cre as a robust pan-pericyte driver for in vivo mutagenesis studies.

This technique is a cost - effective and can be fast alternative for generation of knock down, transgene over expression and reporter lines.

The process used by NIH to select the mouse lines involved a rigorous scientific review process that evaluated information on the knocked out gene, the reliability of the method used to produce the knockout, and whether the mouse line possesses a «reporter» gene, which enables researchers to analyze the pattern of the knockout gene's expression in various mouse tissues.

We are using live imaging, transgenic reporters of osteoblast gene expression and targeted RNAseq approaches to identify the molecular regulators of progressive osteoblast differentiation during expansion of the skull.

B and C, HuH - 7 cells were cotransfected with a p21waf1 / cip1 promoter — driven reporter (p21 - Luc) or its derivatives represented in (A)(0.25 μg of each) together with DDX3 expression construct (pcDNA3 - SRα / FLAG - DDX 3) or control vector (pcDNA3 - SRα / FLAG; 2 μg).

p21waf1 / cip1 promoter — driven luciferase reporter (p21 - Luc; 0.05 - 0.5 μg) and increasing amount (0.1 - 2 μg) of FLAG - DDX3 expression construct were cotransfected into various cell lines as indicated.

To this end, increasing amounts of expression constructs of DDX3 / DQAD, DDX3 / AAA and wild - type DDX3 were cotransfected with p21waf1 / cip1 promoter — driven luciferase reporter.

Expression of HMOX1 E2 - dependent luciferase expression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (FExpression of HMOX1 E2 - dependent luciferase expression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (Fexpression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (Figure 6A).

We found that ~ 90 % knockdown of ABL1 alone resulted in enhanced ABL2 expression and did not produce a significant decrease in the phosphorylation of CrkL, a reporter for the activation state of the ABL kinases (Fig. 2K), and did not inhibit metastasis (Fig. 2, L and M).

Regulation of the expression of human ornithine decarboxylase gene and ornithine decarboxylase promoter - driven reporter gene in transgenic mice

When tested in retinal electroporation studies, DsRed reporter expression was induced in ~ 76 % of GFP + cells, and 100 % of DsRed + cells also expressed GFP.

Under the conditions diagrammed in (A), the Hex Redstar (HexRS) reporter gives a robust readout of anterior endoderm - specific Hex expression.

In both instances, the ICM state appears to express higher levels of Nanog and this observation is consistent with the heterogeneous expression of Nanog reporter ES cells [8], [12].

The detection of this level of Venus expression in the presence of low levels of Hex mRNA suggests that this reporter is indeed extremely sensitive to the low levels of Hex transcript produced in the absence of activin, earlier in differentiation, and in undifferentiated ES cells.

A more recent study showed overlapping expression of nanog with GATA - 6 and a Pdgfra reporter, markers of the primitive endoderm lineage, from the morula to the 64 cell stages [8], suggesting a gradual transition from a pluripotency program to a committed state.

Gene expression Reporter genes, such as luciferase or GFP, can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteration.

In NIH 3T3 cells transfected with PORE reporter and Oct4 expression constructs, overnight treatment with N6 - Bnz - cAMP elicited a response ∼ 50 % of that obtained by treatment with 8 - Br - cAMP, at both the transcriptional (p < 0.001 vs. untreated and vs. 8 - Br - cAMP) and protein levels (Figure 4E).

We will then functionally test the ability of identified sequences to control gene expression during tooth development using transgenic reporter experiments in fish embryos.

The GENSAT project is designed to document the expression patterns of all genes in the nervous system and to generate transgenic mice expressing reporter constructs that recapitulate the authentic expression patterns of selected genes.

Rob Legato, an effects supervisor, told the Hollywood Reporter that in the future, actors involved in big - budget blockbusters might need to have a variety of facial expressions digitally scanned, in case something were to happen.

As Reporters Without Borders announced on their Web site today, Yi's blog was closed down days after being for the BOBs» «freedom of expression» category:

«Following Reporters Without Borders» lead, 25 North American, European, and Australian investment funds and other organizations, who collectively represent over 21 billion dollars under management, have endorsed a joint statement in which they affirm their commitment to freedom of expression on the Internet, and in which they agree, among other things, to monitor business practices being implemented in repressive countries by Internet - sector companies.

Within a day, as they continued to express their thoughts online and on air to reporters, the survivors» expressions of grief turned to calls for political action.

The minor allele of rs25532 significantly decreased luciferase reporter gene expression levels by 15 — 80 %, depending on 5 - HTTLPR allele background and cell type.