Sentences with phrase «external guide sequence»

This additional RNA must contain a sequence complementary to the substrate [external guide sequence (EGS)-RSB- and a 3» - proximal CCA sequence to ensure cleavage.

Any RNA, when in a complex with another oligoribonucleotide known as an external guide sequence (EGS), can become a substrate for ribonuclease P. Simulation of evolution in vitro was used to select EGSs that bind tightly to a target substrate messenger RNA and that increase the efficiency of cleavage of the target by human ribonuclease P to a level equal to that achieved with natural substrates.