Sentences with phrase «extracted nucleic acid»

The TMAdV - specific quantitative real - time PCR was performed on a Stratagene MX3005P real - time PCR system using a 25 µL master mix consisting of 18 µL of water, 2.5 µL of 10X Taq buffer, 1 µL of MgCl2 (50 mM), 0.5 µL of deoxynucleoside triophosphates (dNTPs; 12.5 mM), 0.5 µL of each primer (10 µM), 0.5 µL SYBR green, 0.5 µL of Taq polymerase (Invitrogen, Carlsbad, CA), and 1 µL of extracted nucleic acid.

Extracted nucleic acid was amplified using a random PCR method to generate cDNA libraries for Virochip and deep sequencing analyses as previously described [18], [21].

Not exact matches

Palazzo painstakingly picked out centrosomes from large and easily manipulated surf clam eggs and detected nucleic acids in the concentrated extract.

For each sample type, a standard curve for the TMAdV PCR assay was calculated from 3 PCR replicates at each dilution of nucleic acid extracted from the spiked matrix (data not shown).

For the positive control, nucleic acid extracted from virus stocks was used.

As noted above, existing cancer vaccines that use dendritic cells require extracting cells from a patient's blood, treating them with an engineered protein or nucleic acid that combines tumor antigens with immune - stimulating molecules, and returning the activated dendritic cells to the patient.