Sentences with phrase «fetal calf serum»
The best media available is made from fetal calf serum or horse serum, and you know, that's sort of a nonstarter.
scientificamerican.com
A team of developmental biologists led by Hans Schöler and Karen Hübner at the University of Pennsylvania placed densely packed clusters of stem cells from mouse embryos in a petri dish, using fetal calf serum as a growth medium and adding a gene protein that turns green when germ cells form.
All staining was done at room temperature in FACS Wash Buffer (1 mM EDTA, 2.5 % fetal calf serum in PBS) and all antibodies were from BD Biosciences unless otherwise noted.
In the early 1980s, Eaves, the founding director of the British Columbia Cancer Agency's Terry Fox Laboratory, started buying up the best fetal calf serum by the bucket - full, bringing it to his lab, and rigorously testing it for its ability to support the growth of hematopoietic stem cells.
Dr. Dodds questions why anyone would expect a vaccine ``... with its combo - wombo mixture of antigens and other factors in there — tissue culture remnants, fetal calf serum remnants, adjuvants if it's a killed product — why would you expect all animals to be able to handle that equally?»
2.5 million CD4 + T cells were seeded at a density of 0.8 × 106 cells / ml in RPMI containing 10 % fetal calf serum, 1 % penicillin / streptomycin, and 100 U / ml interleukin - 2 (IL - 2).
High glucose Dulbecco's modified Eagle's medium (DMEM) and fetal calf serum (FCS) were obtained from Invitrogen (Carlsbad, CA, USA).
To make HT22 growth conditioned medium, cells were grown in DMEM with 10 % fetal calf serum.
Briefly, the cerebella of P1 neonatal rats were digested with papain, and dissociated cells were suspended in a synthetic medium containing 1 % fetal calf serum.
For cell culture passage, DEFs were prepared from 12 - day - old duck embryos according to standard protocols, and Vero cells were grown in Dulbecco's modified Eagle's medium (DMEM; Invitrogen) supplemented with 10 % fetal calf serum (FCS), 100 IU / ml penicillin and 100 mg / ml streptomycin.
ES cells were cultured on 0.1 % gelatin - coated flasks or plates (IWAKI) in Glasgow modified Eagle's medium (Gibco) containing non-essential amino - acids, glutamine and sodium pyruvate, 0.1 mM mercaptoethanol, and 10 % Fetal Calf Serum (FCS) together with LIF [30], [60]--[63].
Endogenous peroxidases were inactivated by incubation with 0,3 % hydrogen peroxide dissolved in distilled water for 60 minutes and unspecific binding sites were blocked by incubating the slices in 10 % normal serum dissolved in PBS containing 0,3 % Triton - X100 (PBS - T, Sigma, Deissenhofen, Germany) or in 10 % fetal calf serum (Kraeber, Ellerbek, Germany) for 60 minutes.