Sentences with phrase «fetal fibroblast»

«Advanced Cell Technology has the ability to produce transgenic animals using fetal fibroblast nuclear transfer,» claims Steve Parkinson, president and chief executive officer.

Earlier laboratory studies had shown that fetal fibroblasts, closely related cells from the fetus, respond to tamoxifen by producing a natural peptide called transforming growth factor beta - 1 (TGF beta - 1).

The other clones came from fetal fibroblasts.

Thomson's group also created human iPSCs, but used fetal fibroblasts and foreskin fibroblasts and a different set of proteins; while both groups used Oct - 4 and Sox2, Thomson's group used Nanog and Lin28 instead of Klf4 and c - Myc (Yu et al., 2007).

Goat fetal fibroblasts were isolated as described previously [22] and cultured in DMEM supplemented with 15 % FBS, 1 % sodium pyruvate and 1 % penicillin streptomycin to achieve 80 — 90 % confluency on the day of transfection.

Not exact matches

↵ 3 The abbreviations used are: HUVEC, human umbilical vein endothelial cell; DiI - Ac - LDL, 1,1 ′ - dioctadecyl - 3,3,3 ′, 3 ′ - tetramethyl - indocarbocyanine acetylated low - density lipoprotein; FACS, fluorescence - activated cell sorting; FGFR, fibroblast growth factor receptor; Flk, fetal liver kinase; ICAM, intercellular adhesion molecule; mAb, monoclonal antibody; PE, phycoerythrin; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule.

Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin - coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer's instructions.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

This induced pluripotent stem cell (iPSC) line was generated from IMR90 fetal lung fibroblasts by viral transduction of a combination of OCT4, SOX2, NANOG, and LIN28 genes.

But initial experiments on human fibroblasts from three sources — fetal heart cells, embryonic stem cells and neonatal skin cells — revealed that the GMT combination alone was not sufficient.