Mutations for mammoth hemoglobin, extra hair growth, fat production, down to nuanced climate adaptations such as slightly altered sodium ion channels in cell membranes have already been engineered into
fibroblast cell lines.
If fibroblasts could be used to create baby birds then the world of bird de-extinction would open up expansively, and the frozen
fibroblast cell lines of endangered bird species at the San Diego Frozen Zoo could be used to rescue species on the verge of extinction.
One of the many payoffs of the International HapMap Project was that it characterized genetic variation in
fibroblast cell lines that could be ordered from Coriell for subsequent experiments.
Not exact matches
So Welham's team set out to engineer vocal fold mucosae in the lab using the two main
cell types that make up the tissue — connective
fibroblasts, which form the main body, and epithelial
cells, which
line the surface.
Instead of dispensing ink, Organovo's bioprinter uses two robotic tips to deposit globs of
cells — in this case, endothelial
cells that
line blood - vessel walls, smooth muscle
cells that regulate vessel dilation and contraction, and structural
fibroblast cells.
The inflammatory processes also activate
fibroblast - like synoviocytes (FLS), specialized
cells that
line the inside of joints providing lubrication and repairing joint injuries.
Much like metastasizing tumor
cells, the team found, the
fibroblasts can wriggle through the
cells that
line and protect blood vessels.
In their new study, co-corresponding authors Wei Wang, PhD, professor in the departments of Chemistry and Biochemistry and Cellular and Molecular Medicine, and Gary S. Firestein, MD, professor in the Department of Medicine, investigated epigenetic patterns in
fibroblast - like synoviocytes (FLS)-- a specialized type of
cell that
lines the inside of joints.
Individual cultures of 3T3 (a murine
fibroblast line) and endothelioma
cells subjected to identical treatment were used for negative and positive controls, respectively.
In parallel, we have generated iPS
cell lines from
fibroblasts of two patients (Coriell) suffering from Leber Congenital Amaurosis (LCA).
Similarly, biopsies are obtained from many subjects and skin
cells (
fibroblasts) are isolated from these specimens, dedifferentiated to iPS
lines, and reprogrammed and studied as neural and glial
lines in culture.
To explore the biological function of DDX3 on
cell growth control, a colony formation assay was done in several tumor
cell lines (HuH - 7, HCT116, and HeLa) and normal murine
fibroblast cells (NIH 3T3) under the selection of G418.
HuH - 7 (human HCC), HepG2 (human hepatocellular blastoma), 293T (human embryonic kidney
fibroblast), HeLa (human cervical cancer
cells), NIH 3T3 (murine
fibroblast), and HCT116 (human colon cancer) and its derived
cell line HCT116 p21 − / − (kindly provided by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD; ref.
Citation: Li C, Yu H, Ma Y, Shi G, Jiang J, Gu J, et al. (2009) Germline - Competent Mouse - Induced Pluripotent Stem
Cell Lines Generated on Human
Fibroblasts without Exogenous Leukemia Inhibitory Factor.
Most prior established iPS
cell lines were derived and maintained on mouse embryonic
fibroblast (MEF)
cells supplemented with exogenous leukemia inhibitory factor (LIF).
In mouse embryo
fibroblasts (MEFs)[2], as well as in the PCa
cell lines [unpublished data], DAXX represses expression of these genes via mechanisms that include its binding to DNA methyl transferases (DNMTases) and hypermethylation of the promoter regions of the corresponding genes [2].
Furthermore, using a phospho - tyrosine [Y] 474 (in - house generated) and a phospho - serine [S] 176 (
Cell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right pan
Cell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK
fibroblast and HCT116 colon cancer epithelial
cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer
cell line SW480 (Fig. 3E, right pan
cell line SW480 (Fig. 3E, right panel).
To further facilitate genome editing in pigs, we report here establishment of a pig
line with Cre - inducible Cas9 expression that allows a variety of ex vivo genome editing in
fibroblast cells including single - and multigene modifications, chromosome rearrangements, and efficient in vivo genetic modifications.
ACTB mRNA expression levels in lymphoblastic
lines and
fibroblasts derived from affected individuals were decreased in comparison to those in control
cells.
We derived a
fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of
cell surface markers commonly expressed on pluripotent stem
cells (Figure 1B, C and D).
Leonard Hayflick, Ph.D., and Paul S. Moorhead, Ph.D, established the WI - 38
cell line of human diploid
fibroblasts in 1962.
This induced pluripotent stem
cell (iPSC)
line was generated from IMR90 fetal lung
fibroblasts by viral transduction of a combination of OCT4, SOX2, NANOG, and LIN28 genes.