Sentences with phrase «fibroblast cells as»

The potential of iPS cells to help treat everything from damaged heart tissue to Parkinson's disease, has prompted intensive research that has looked into the use of skin fibroblast cells as an alternative to controversial embryonic stem cells.

Special focus is placed on Rho - kinase inhibition, relating to its potential to promote and support extracellular matrix production in cultured chondrocytes and its role in fibroblast cells as a part of direct chemical cellular differentiation.

Not exact matches

The team took small pieces of ear tissue from XXY and XYY mice, cultured them, and collected connective tissue cells known as fibroblasts.

It takes far more expertise, however, to remove dozens of PERV genes at once, as eGenesis does in pig fibroblasts, which are connective - tissue cells.

To find out, John O'Neill, a biologist at the Medical Research Council's Laboratory of Molecular Biology in Cambridge, U.K., and his team studied skin cells known as fibroblasts, which are essential for wound healing.

Importantly, the new technique grows the pluripotent stem cells as floating spheres in high concentrations of two growth factors, fibroblast growth factor - 2 and epidermal growth factor.

Two months after therapy, tissue analysis showed less scarring and higher levels of regenerative agents such as fibroblast growth factor — which accelerate healing — in rats treated with SIS plus stem cells compared with those treated with SIS alone.

They start by harvesting skin cells known as fibroblasts and growing these in a sheet.

To find out, Deb and his co-authors genetically tagged cardiac fibroblasts in mice and watched as they transitioned into bone - forming, osteoblast - like cells after heart injury.

The cells types — known as Hofbauer cells, cytotrophoblasts, and fibroblasts — were obtained from normal term pregnancies.

It spurred the fastest growth of fibroblast cells, with 17 per cent more cells formed after six days as in a control culture.

The tissue, called the stroma, includes fat cells, or adipocytes, that provide padding; fibroblasts, which make the framework for tissue; pericytes in blood vessels, which are contractile cells that help regulate blood pressure; as well as myoepithelial cells comprising the outer layer of the ductal system through which milk flows.

The experiments, including studies conducted in a bioengineered 3 - D model of lung tissue seeded with relevant cells, showed that CHI3L1 regulates a pathway that recruits cells such as macrophages and fibroblasts that produce the scarring, or fibrosis.

As controls, fibroblasts and secretions from normal lab rats, mice, and another rodent called the spiny mouse were powerless to stop the human cancer cells growing.

When the researchers eliminated the expression of this gene in the CAFs, these cells behaved like normal fibroblasts, as they lost the ability to help the tumour cells to progress.

It's a «very important» and «very thorough» paper, says James Woods, a microbiologist at Midwestern University in Downers Grove,, Illinois, who has studied the migration of RASFs: «The fibroblasts that were active, they are usually thought of as a resident [i.e., nonmobile] cell.

Basically, when you look at different types of cells, such as fibroblasts, which form connective tissue, or epithelial cells, from saliva, you see general correlations within a person.

The researchers hypothesized that these free - floating copies of the receptor would serve as decoys, capturing fibroblast growth factor molecules and reducing stimulation of the cartilage cells» receptors.

When stimulated by molecules called fibroblast growth factors, this receptor, known as FGFR3, prevents the cells from maturing and impedes bone formation.

Periostin (POSTN) was identified by microarray RNA profiling studies as a stromal factor involved in maintaining the normal stem cell niche and demonstrated to be secreted by stromal fibroblasts, but not by infiltrating tumor cells (Malanchi et al., 2012).

Cells were washed once with serum - free DMEM and then incubated in 10 % DMEM containing 10 μg / ml DiI - Ac - LDL for 4 h. Endothelioma cells and 3T3 fibroblast cells were used as a positive and negative reference, respectiCells were washed once with serum - free DMEM and then incubated in 10 % DMEM containing 10 μg / ml DiI - Ac - LDL for 4 h. Endothelioma cells and 3T3 fibroblast cells were used as a positive and negative reference, respecticells and 3T3 fibroblast cells were used as a positive and negative reference, respecticells were used as a positive and negative reference, respectively.

Indeed, they were: According to the team's findings, adipose stem cells can be turned into iPS cells twice as quickly as fibroblast skin cells and with 20 times the efficiency.

Xu and his team, which includes researchers from Penn's departments of Dermatology and Biology, as well as the New Jersey Institute of Technology, started with human skin cells called dermal fibroblasts.

Human embryonic stem cells derived from affected embryos during a pre-implantation diagnostic (PGD), as well as the conversion of somatic cells, such as skin fibroblasts, into induced pluripotent stem cells by genetic manipulation, offer the unique opportunity to have access to a large spectrum of disease - specific cell models.

Similarly, biopsies are obtained from many subjects and skin cells (fibroblasts) are isolated from these specimens, dedifferentiated to iPS lines, and reprogrammed and studied as neural and glial lines in culture.

Review conceptual and technical aspects of direct reprogramming of somatic cells, such as fibroblasts and astrocytes, into induced neurons.

Unlike highly specialized skin - cell fibroblasts, these cells in the fat have a relatively wide portfolio of differentiation options — becoming fat, bone or muscle as needed.

Moreover, there are yet other cell types — such as visceral adipose tissue macrophages and cytotoxic CD8 + T - cells — in which the age - related supernumerary accumulation of dysfunctional and apoptosis - resistant cells appears to play a highly deleterious role on tissue function, but where the cells are not «senescent» cells in the classical sense of p16Ink4a expression and the senescence - associated secretory profile observed in senescent fibroblasts.

Yamanaka's group used human adult dermal fibroblasts and induced them to become iPSCs, appearing and functioning like hESCs, by having them express the same proteins as he used with mouse cells: Oct - 4, Sox2, Klf4, and c - Myc (Takahashi et al., 2007).

We prefer to culture our cells on mouse embryo fibroblasts (MEFs) but occasionally grow the cells feeder free for particular applications, such as nucleofection, viral transduction or karyotyping.

Skin substitutes involve different cell composites, such as: fibroblasts, keratinocytes, epithelial cells and / or MSCs transplanted on membranes or with supportive matrix.

The systematic characterization of somatic mutations fed nicely into a phylogenetic analysis, which revealed that all d42m1 derived clones were related (genetically speaking) to one another, while quite distinct from the other sarcoma (H31m1) as well as normal fibroblast cells.

As bioinformatics approaches can only provide partial solutions, a functional analysis pipeline, based on primary (fibroblast) cell cultures, induced Pluripotent Stem cells (iPS) or simple model organisms, is truly essential.

For instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS cells.

In the current issue of the journal Developmental Cell, the scientists show that the secret to this switch lies in the cells that surround the muscle cells, known as fibroblasts, which send signals that tell cardiomyocytes to divide or get bigger in size.

As part of this global aim we have demonstrated that one of the most abundant stromal populations, the cancer associated fibroblasts (CAFs), can sample and present tumour antigen to T cells in an antigen specific - antigen dependent manner resulting in T cell deletion.

The flat elongated cells are fibroblasts used as «feeder cells.»

(C) When iPS cells were cultured in the absence of the HFF cells, various differentiated cells could be observed, such as definitive endoderm - like cells (a) and (b), fibroblast - like cells (c), cardiomyocytes (d), epidermis - like cells (e) and neural rosettes (f).

Normal human lung fibroblasts (NHLF) cells grown in 384 - well Optilux plates were treated with candidate compounds and incubated for 18 hours prior to fixation and staining with Hoescht dye and anti-HMOX1 antibody as described in Materials and Methods.

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

The hESC colonies were mechanically divided and seeded as small aggregates onto 12 - well plates (CellBIND Surface, Corning, Inc., Corning, NY), containing gamma - irradiated human foreskin fibroblasts (CRL - 2429, ATCC, Manassas, VA) as feeder cells.

A wound - healer run amok In the 1970s, a scientist at the University of Geneva in Switzerland discovered cells in healing wounds that seemed to be intermediate in character between fibroblasts, which secrete fibers such as collagen that make up the matrix that holds cells together in tissues, and smooth muscle cells, like those in the intestines and blood vessels.

In mouse embryo fibroblasts (MEFs)[2], as well as in the PCa cell lines [unpublished data], DAXX represses expression of these genes via mechanisms that include its binding to DNA methyl transferases (DNMTases) and hypermethylation of the promoter regions of the corresponding genes [2].

These scientific goals guide our efforts at determining the role and mechanisms of the stromal cells, including the fibroblasts, endothelial and lymphatic elements, the matrix and the inflammatory / immune cells as critical targets for therapeutic intervention.

Mutations for mammoth hemoglobin, extra hair growth, fat production, down to nuanced climate adaptations such as slightly altered sodium ion channels in cell membranes have already been engineered into fibroblast cell lines.

«Our findings here serve as a proof of concept that human fibroblasts can be reprogrammed successfully into beating heart cells.»

In 2012, Dr. Srivastava and his team reported in the journal Nature that fibroblasts could be reprogrammed into beating heart cells by injecting just three genes, together known as GMT, into the hearts of live mice that had been damaged by a heart attack.

HIF1 - alpha functions as a tumor promoter in cancer associated fibroblasts, and as a tumor suppressor in breast cancer cells: Autophagy drives compartment - specific oncogenesis

Methods to enhance the reprogramming efficiency would significantly increase the feasibility of this approach, especially for cell types which tend to be more difficult to reprogram, such as the primary adult human fibroblasts used in this study.

It not only promotes fibroblast proliferation (fibroblasts are the cells that produce collagen and other fibers), but it also acts as an assistant (a «cofactor») in enzymatic activity that relates directly to skin health and function.