Sentences with phrase «fibroblast cells into»

They have already shown that they can transform human fibroblast cells into pluripotent stem cells, and now plan to start working on delivering the proteins needed to differentiate stem cells into specialized tissues.

Not exact matches

They turned the fibroblasts into stem cells and noticed that in the process, some of the cells lost the extra sex chromosome.

The team also transplanted treated fibroblasts into the hearts of live mice, where they developed into cardiomyocytes (Cell, DOI: 10.1016 / j.cell.2010.07.002).

Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

The study team removed fibroblasts (skin cells) from DBA patients, and in cell cultures, using proteins called transcription factors, reprogrammed the cells into iPSCs.

Hayashi then drew on other research showing that one key regulatory molecule (activin A) and a growth factor (basic fibroblast growth factor) could convert cultured early embryonic stem cells into cells akin to epiblasts.

To find out, Deb and his co-authors genetically tagged cardiac fibroblasts in mice and watched as they transitioned into bone - forming, osteoblast - like cells after heart injury.

The orbital remodeling in TED is likely induced by infiltrating T lymphocytes and mast cells, which activate orbital fibroblast effector cells to either proliferate and form scar - producing cells called myofibroblasts or turn into pro-inflammatory fat cells.

In the online edition of Science, he and his colleagues report that Oct4 and Sox2 were capable of converting neonatal foreskin fibroblasts into cells similar to Yamanaka's, whereas Nanog significantly boosted the frequency of reprogramming and Lin28 upped it by a moderate amount.

Researchers from the UNC School of Medicine have discovered that cells called fibroblasts, which normally give rise to scar tissue after a heart attack, can be turned into endothelial cells, which generate blood vessels to supply oxygen and nutrients to the injured regions of the heart, thus greatly reducing the damage done following heart attack.

Cells called cancer - associated fibroblasts arrange a normal meshwork of fibers into straight tracks, cell biologist Begum Erdogan of Vanderbilt University in Nashville and colleagues reported December 13 at the annual meeting of the American Society for Cell Biolcell biologist Begum Erdogan of Vanderbilt University in Nashville and colleagues reported December 13 at the annual meeting of the American Society for Cell BiolCell Biology.

That is, instead of just 30 percent of fibroblasts naturally switching into endothelial cells, 60 percent made the switch.

«It appears that injury itself can induce fibroblasts to change into endothelial cells so the heart heals better.

But Erdogan and colleagues found that fibroblasts associated with prostate cancer could either lay straight new fibronectin tracks or grab and pull snarled networks into straightaways that cancer cells could then move along.

The green blood vessels running horizontally are partly composed of red / yellow cells, suggesting that fibroblasts are incorporated into blood vessels.

Researchers used the CRISPR / Cas9 gene editing technique to introduce a segment of a human gene causing Huntington's, with a very long glutamine repeat region, into pig fibroblast cells.

For the new study, the team used a cell - reprogramming technique (similar to those used to reprogram skin cells into stem cells) to generate human DRG - type sensory neurons from ordinary skin cells called fibroblasts.

Following a cut to the skin, fibroblast cells rush to the wound and secrete a matrix that helps skin cells move into place, grow, and heal the injury.

• In March, a University of Wisconsin team reprogrammed skin fibroblasts into embryonic stem cells without incorporating the viral or other foreign DNA that can lead to complications like cancer.

In 1963 Pietro Angeletti and one of us (Levi Montalcini), who were then at the Istituto Superiore di Sanità in Rome, dissected sensory and sympathetic ganglia into their cellular components: neurons, glial cells (which support and nourish the neurons) and fibroblasts (embryonic connective - tissue cells).

Indeed, they were: According to the team's findings, adipose stem cells can be turned into iPS cells twice as quickly as fibroblast skin cells and with 20 times the efficiency.

The potential of iPS cells to help treat everything from damaged heart tissue to Parkinson's disease, has prompted intensive research that has looked into the use of skin fibroblast cells as an alternative to controversial embryonic stem cells.

They found that another triad of transcription factors succeeded in converting fibroblasts into green fluorescent cells.

Human embryonic stem cells derived from affected embryos during a pre-implantation diagnostic (PGD), as well as the conversion of somatic cells, such as skin fibroblasts, into induced pluripotent stem cells by genetic manipulation, offer the unique opportunity to have access to a large spectrum of disease - specific cell models.

The Joslin researchers «reprogrammed» the fibroblasts into iPSCs by using viral procedures that activated four genes that together maintain cells in the iPSC state.

It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell - like state by introducing combinations of four transcription factors.

Review conceptual and technical aspects of direct reprogramming of somatic cells, such as fibroblasts and astrocytes, into induced neurons.

We have leveraged this knowledge to reprogram fibroblasts directly into cardiomyocyte - like cells for regenerative purposes.

Because of its success with the fibroblasts, MyoD was subsequently used in many other reprogramming studies to see what other cells it could make into muscle.

Recently, Dr. Ding achieved entirely gene - free chemical reprogramming, using only drugs to turn fibroblasts directly into brain cells.

To convert the fibroblasts into muscle cells, the researchers transfected the fibroblasts with the cDNA of MyoD, forcing the cells to express MyoD (Davis et al., 1987).

Using unique drug cocktails, stem cell scientist Sheng Ding, PhD, a senior investigator at the Gladstone Institutes, can transform fibroblasts (skin cells) into fully functional brain, heart, liver, and insulin - producing pancreas cells.

Dr. Ding's work revolves around chemical reprogramming — the use of small molecule drug mixtures to coax fibroblasts into becoming a variety of other cell types.

In his laboratory mice, Dr. Ding has now used chemical reprogramming to turn fibroblasts into neural «precursor» cells with the potential to become new oligodendrocytes.

His research is focused on retinal regeneration by reprogramming human fibroblasts either into induced pluripotent stem cells or directly into photoreceptors.

Just this year, another group found that fibroblast cells (cells active in connective tissue) taken from mouse tails could be made into nerve cells, or neurons.

In mice, induced pluripotent stem cells created from fibroblasts have been reprogrammed to develop into both sperm and egg cells and have yielded healthy offspring.

For example, we are reprogramming cardiac fibroblasts — the heart's connective tissue — directly into beating cardiac - muscle cells.

Yangqing et al recently had success in pursuing this pathway — turning chicken fibroblasts into «germ - like» cells in the same way that induced pluripotent stem cells are made2.

The systematic characterization of somatic mutations fed nicely into a phylogenetic analysis, which revealed that all d42m1 derived clones were related (genetically speaking) to one another, while quite distinct from the other sarcoma (H31m1) as well as normal fibroblast cells.

The DNA was then incorporated into the genome of the fibroblasts and translated into protein by the host cell.

When transplanted into mouse hearts 1 day after the three factors were introduced, fibroblasts turned into cardiomyocyte - like cells within the beating heart.

Studies on interactions among tumor cells, fibroblasts and endothelial cells are also done in 3 - D models, in which cells are embedded into collagen to mimic the tumor microenvironment.

Oct4, Sox2, c - Myc, and Klf4 were the four original factors that were capable of converting mouse and human fibroblasts into iPs cells [1], [3], [5], [11], [12].

Remarkably, a combination of just three of the factors (Gata4, Mef2c, and Tbx5) was enough to efficiently convert fibroblasts into cells that could beat like cardiomyocytes and turned on most of the same genes expressed in cardiomyocytes.

Since murine NSCs have been «primed» with several of the factors originally discovered to reprogram fibroblasts into iPS cells, they represent an attractive source of starting material for iPS cell induction studies.

For the first time, scientists have turned muscular dystrophy patients» fibroblast cells (common cells found in connective tissue) into stem...

The researchers then successfully prodded what they call keratinocyte - derived iPS cells or KiPS cells to distinguish them from fibroblast - derived iPS cells into becoming all the cell types in the human body, including heart muscle cells and dopamine - producing neurons, which are affected by Parkinson's disease.

Then, by treating the fibroblasts with a unique «cocktail» of molecules and reprogramming factors, they transformed the cells into endoderm - like cells.