Sentences with phrase «fibroblast cells using»

Briefly, the retrovirus was transfected into DF1 chicken fibroblast cells using Superfect (Qiagen, Gaithersburg, MD) and viral particles were harvested over 1 week.

Not exact matches

Using patient's own tissue and specific combination of reprogramming factors, skin fibroblasts are successfully converted to cell type that... Continue reading Breakthrough Device Looks to Heal Organs

The production of virus - free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine.

Lee's team used a retrovirus to transfer the fluorescent gene to dog fibroblast cells, but they could not control where the virus inserted the gene.

The researchers found that, in response to the maximum tolerated doses of several commonly used chemotherapy drugs, breast cancer - associated fibroblasts secrete large amounts of cell signaling proteins called ELR + chemokines.

«Advanced Cell Technology has the ability to produce transgenic animals using fetal fibroblast nuclear transfer,» claims Steve Parkinson, president and chief executive officer.

The study team removed fibroblasts (skin cells) from DBA patients, and in cell cultures, using proteins called transcription factors, reprogrammed the cells into iPSCs.

So Welham's team set out to engineer vocal fold mucosae in the lab using the two main cell types that make up the tissue — connective fibroblasts, which form the main body, and epithelial cells, which line the surface.

To make the pigmented skin constructs, the team used three different types of skin cells — keratinocytes, melanocytes, and fibroblasts — and a two - step «drop on demand» bioprinting method.

In vitro experiments used human colon carcinoma cells with and without MMP9 and mouse embryonic fibroblasts, which are connective tissue cells that make the extracellular matrix and collagen and play an important role in tissue repair.

Researchers used the CRISPR / Cas9 gene editing technique to introduce a segment of a human gene causing Huntington's, with a very long glutamine repeat region, into pig fibroblast cells.

The study carried out at the University of Eastern Finland used the induced pluripotent stem cell technology, which enables the generation of pluripotent stem cells from human skin fibroblasts.

Instead of dispensing ink, Organovo's bioprinter uses two robotic tips to deposit globs of cells — in this case, endothelial cells that line blood - vessel walls, smooth muscle cells that regulate vessel dilation and contraction, and structural fibroblast cells.

For the new study, the team used a cell - reprogramming technique (similar to those used to reprogram skin cells into stem cells) to generate human DRG - type sensory neurons from ordinary skin cells called fibroblasts.

The authors used induced pluripotent stem cells (iPSCs) generated from the fibroblasts of both healthy individuals (WT - iPSC) and TD patients (TD - iPSC).

These genes all help control actin, a protein used by fibroblast cells to move.

This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 + endothelial cells, and CD45 + immune cells isolated from lungs of mice with macrometastases, which is a replication of the experiment reported in Figure 2H.

↵ 3 The abbreviations used are: HUVEC, human umbilical vein endothelial cell; DiI - Ac - LDL, 1,1 ′ - dioctadecyl - 3,3,3 ′, 3 ′ - tetramethyl - indocarbocyanine acetylated low - density lipoprotein; FACS, fluorescence - activated cell sorting; FGFR, fibroblast growth factor receptor; Flk, fetal liver kinase; ICAM, intercellular adhesion molecule; mAb, monoclonal antibody; PE, phycoerythrin; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule.

Individual cultures of 3T3 (a murine fibroblast line) and endothelioma cells subjected to identical treatment were used for negative and positive controls, respectively.

Cells were washed once with serum - free DMEM and then incubated in 10 % DMEM containing 10 μg / ml DiI - Ac - LDL for 4 h. Endothelioma cells and 3T3 fibroblast cells were used as a positive and negative reference, respectiCells were washed once with serum - free DMEM and then incubated in 10 % DMEM containing 10 μg / ml DiI - Ac - LDL for 4 h. Endothelioma cells and 3T3 fibroblast cells were used as a positive and negative reference, respecticells and 3T3 fibroblast cells were used as a positive and negative reference, respecticells were used as a positive and negative reference, respectively.

The potential of iPS cells to help treat everything from damaged heart tissue to Parkinson's disease, has prompted intensive research that has looked into the use of skin fibroblast cells as an alternative to controversial embryonic stem cells.

First, the MimEX ™ Cell Plating Procedure is used to maintain a uniform irradiated fibroblast monolayer for the rapid clonal expansion and efficient differentiation of tissue - specific adult epithelial stem cells.

The Joslin researchers «reprogrammed» the fibroblasts into iPSCs by using viral procedures that activated four genes that together maintain cells in the iPSC state.

Mechanical properties of many different cell types including glial cells, platelets, cardiocytes, macrophages, endothelial cells, epithelial cells, fibroblasts, bladder cells and more have been determined using F - d spectroscopy.

Because of its success with the fibroblasts, MyoD was subsequently used in many other reprogramming studies to see what other cells it could make into muscle.

Recently, Dr. Ding achieved entirely gene - free chemical reprogramming, using only drugs to turn fibroblasts directly into brain cells.

Using unique drug cocktails, stem cell scientist Sheng Ding, PhD, a senior investigator at the Gladstone Institutes, can transform fibroblasts (skin cells) into fully functional brain, heart, liver, and insulin - producing pancreas cells.

Dr. Ding's work revolves around chemical reprogramming — the use of small molecule drug mixtures to coax fibroblasts into becoming a variety of other cell types.

In his laboratory mice, Dr. Ding has now used chemical reprogramming to turn fibroblasts into neural «precursor» cells with the potential to become new oligodendrocytes.

Yamanaka's group used human adult dermal fibroblasts and induced them to become iPSCs, appearing and functioning like hESCs, by having them express the same proteins as he used with mouse cells: Oct - 4, Sox2, Klf4, and c - Myc (Takahashi et al., 2007).

If fibroblasts could be used to create baby birds then the world of bird de-extinction would open up expansively, and the frozen fibroblast cell lines of endangered bird species at the San Diego Frozen Zoo could be used to rescue species on the verge of extinction.

For instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS cells.

The flat elongated cells are fibroblasts used as «feeder cells.»

JOE PALCA: Well, John, what they did was they - instead of starting with embryos and using those to derive embryonic stem cells, they started with skin cells, or actually precursors to skin cells called fibroblast, that we all have in our skin.

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

While this manuscript was in preparation, a similar episomal strategy was used to reprogram human primary fibroblasts using a distinct pluripotent cocktail of factors, validating our methods, but with significant lower efficiency (only 3 to 6 colonies / 106 input cells)[42].

Furthermore, using a phospho - tyrosine [Y] 474 (in - house generated) and a phospho - serine [S] 176 (Cell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right panCell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right pancell line SW480 (Fig. 3E, right panel).

Our goal is to determine if epithelial cells migrate passively because of the pushing force generated by cell division in the crypts, actively using specialized cellular protrusions or if they are transported by underlying fibroblasts and basement membrane (BM).

Here the authors, using an ex-vivo model, show that cancer - associated fibroblasts facilitate colon cancer cells invasion in a matrix metalloproteinase - independent manner, likely by pulling and stretching the basement membrane to form gaps.

A combination of markers was used to discriminate fibroblasts from other cell types to validate the purity of isolated cells and to discriminate NAFs from CAFs.

However, research in Dr. King's lab has suggested a potentially more effective therapy that uses connective - tissue cells called fibroblasts.

The single - gene knockout fibroblasts were successfully used for somatic cell nuclear transfer (SCNT) and resulted in live - born goats harboring biallelic mutations.

Using human colon cancer cells and primary human fibroblasts isolated from tumors and adjacent normal tissues, Alexandros Glentis and colleagues at the Institut Curie addressed the question of whether the cancer cells or the CAF cells were responsible for the breakdown of the basement membrane that leads to cancer progression.

(A) The expression patterns of germ cell marker genes in monkey testis (5 years old), mouse embryonic fibroblast (MEF), monkey ES cells (ES), and developing EBs (days 3, 7, 14, 21, and 28) were examined using an RT - PCR analysis.

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

In their study, published alongside Deng's in Cell Stem Cell, the researchers converted human fibroblasts to neurons using a combination of seven molecules applied to the cells for one week, followed by a three - week maturation period in a medium containing two of the reprogramming molecules and one other molecule.

Cells were stained in solution using a mixture of GCTM - 2 (mouse IgM), TG30 (anti-CD9, mouse IgG2a) and Thy1.2 - PE (to gate out any mouse embryonic fibroblasts, BD Bioscience, cat.

Methods to enhance the reprogramming efficiency would significantly increase the feasibility of this approach, especially for cell types which tend to be more difficult to reprogram, such as the primary adult human fibroblasts used in this study.

She recommends Clarins Multi-Active Day ($ 54, clarinsusa.com), which uses an encapsulated teasel extract that targets the key youth cell of the skin (called a fibroblast).