Sentences with phrase «fibroblast cells with»

Imagine researchers» excitement, then, when Kyoto University's Shinya Yamanaka reported last year that his group had created embryolike cells directly, without transferring chromosomes, simply by injecting mouse fibroblast cells with four genes that are active in embryonic cells but not in adult ones.

Not exact matches

In a preliminary experiment, the team found that stem cells produced from fibroblasts of men with Klinefelter syndrome also lost the extra sex chromosome.

Working with human breast tissue, the new study's authors attempted to induce EMT in normal cells; they figured they would just get fibroblasts, a type of connective tissue that is important in wound healing.

So Izpisúa Belmonte and his colleagues harvested fibroblasts, which are far more common than stem cells, from the skin of people with the bone marrow disease Fanconi anaemia.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

When cells that produced IL - 21 were put in culture with synovial fibroblasts (which are the main contributors to joint inflammation in rheumatoid arthritis), they induced the production of proinflammatory cytokines by these synovial fibroblasts, and cells that do not produce IL - 21, did not demonstrate this same outcome.

Two months after therapy, tissue analysis showed less scarring and higher levels of regenerative agents such as fibroblast growth factor — which accelerate healing — in rats treated with SIS plus stem cells compared with those treated with SIS alone.

In studies with human fibroblasts that make up connective tissue, Boger's team tested whether NOD1 activity could affect CMV replication in cultures of cells grown in the lab.

Hamlyn, working with Richard Schmidt of the School of Pharmacy at the University of Wales in Cardiff, exposed human fibroblast cells to six fungi strains.

It spurred the fastest growth of fibroblast cells, with 17 per cent more cells formed after six days as in a control culture.

The experiments, including studies conducted in a bioengineered 3 - D model of lung tissue seeded with relevant cells, showed that CHI3L1 regulates a pathway that recruits cells such as macrophages and fibroblasts that produce the scarring, or fibrosis.

But Erdogan and colleagues found that fibroblasts associated with prostate cancer could either lay straight new fibronectin tracks or grab and pull snarled networks into straightaways that cancer cells could then move along.

In vitro experiments used human colon carcinoma cells with and without MMP9 and mouse embryonic fibroblasts, which are connective tissue cells that make the extracellular matrix and collagen and play an important role in tissue repair.

Researchers used the CRISPR / Cas9 gene editing technique to introduce a segment of a human gene causing Huntington's, with a very long glutamine repeat region, into pig fibroblast cells.

Compared with cells in the center, cells along the edge experienced greater mechanical stress and tension, and appeared more like fibroblasts, which form the collagen of connective tissue.

An example of this capability can be visualized in movie 1, where a fibroblast is imaged before and after treatment with insulin hormone, which intensely enhances the ruffling at the leading edge of the cell.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 + endothelial cells, and CD45 + immune cells isolated from lungs of mice with macrometastases, which is a replication of the experiment reported in Figure 2H.

The experiment shows that β1 - integrin — one of 28 types of integrin — maintains a link between the stem cell and its environment, and interacts biochemically with a growth factor called fibroblast growth factor [FGF] to promote stem cell growth and restoration after muscle tissue injury.

Metastatic variant of MCF7 (MCF7 - BoM2d cells) was suspended in DMEM - F12 medium supplemented with 2 % of B27 supplement, 0.4 % BSA, 4 μg / mL insulin, 20 ng / mL basic fibroblast growth factor (bFGF), and 20 ng / mL EGF (Invitrogen).

Cells were washed once with serum - free DMEM and then incubated in 10 % DMEM containing 10 μg / ml DiI - Ac - LDL for 4 h. Endothelioma cells and 3T3 fibroblast cells were used as a positive and negative reference, respectiCells were washed once with serum - free DMEM and then incubated in 10 % DMEM containing 10 μg / ml DiI - Ac - LDL for 4 h. Endothelioma cells and 3T3 fibroblast cells were used as a positive and negative reference, respecticells and 3T3 fibroblast cells were used as a positive and negative reference, respecticells were used as a positive and negative reference, respectively.

The research team, spearheaded by postdoctoral fellow Yajie Liang, Ph.D., wondered whether the cells» survival could also be enhanced with steady doses of a compound called basic fibroblast growth factor (bFGF), an «energy drink» that spurs cells to grow.

Fibroblasts are a dynamic cell type that achieve selective differentiated states to mediate acute wound healing and long - term tissue remodeling with scarring.

Indeed, they were: According to the team's findings, adipose stem cells can be turned into iPS cells twice as quickly as fibroblast skin cells and with 20 times the efficiency.

Xu and his team, which includes researchers from Penn's departments of Dermatology and Biology, as well as the New Jersey Institute of Technology, started with human skin cells called dermal fibroblasts.

(B) Transmission electron micrograph showing an interstitial fibroblast - like cell packed with mitochondria (arrow) with dense cristae and small lipid droplets in the rope tissue.

The scientists then looked at gene activation in insulin signaling pathways for iPSCs and fibroblasts with IR mutations, and for corresponding cells derived from people without those mutations.

MAIT cells are activated and secrete Interferon - g after stimulation by fibroblasts or antigne presenting cells co-cultured with bacteria and yeasts but not viruses.

Because of its success with the fibroblasts, MyoD was subsequently used in many other reprogramming studies to see what other cells it could make into muscle.

To convert the fibroblasts into muscle cells, the researchers transfected the fibroblasts with the cDNA of MyoD, forcing the cells to express MyoD (Davis et al., 1987).

Cells with average speed represented cells with a spindle - like fibroblast shape, medium size, and a percentage of senescent cells in - between the fast and slow populaCells with average speed represented cells with a spindle - like fibroblast shape, medium size, and a percentage of senescent cells in - between the fast and slow populacells with a spindle - like fibroblast shape, medium size, and a percentage of senescent cells in - between the fast and slow populacells in - between the fast and slow population.

SPARC regulates collagen interaction with cardiac fibroblast cell surfaces.

In his laboratory mice, Dr. Ding has now used chemical reprogramming to turn fibroblasts into neural «precursor» cells with the potential to become new oligodendrocytes.

Yamanaka's group used human adult dermal fibroblasts and induced them to become iPSCs, appearing and functioning like hESCs, by having them express the same proteins as he used with mouse cells: Oct - 4, Sox2, Klf4, and c - Myc (Takahashi et al., 2007).

Valdivia submitted this image of mouse embryonic fibroblasts forming focal adhesions, points of contact of the cell with the extracellular matrix.

Skin substitutes involve different cell composites, such as: fibroblasts, keratinocytes, epithelial cells and / or MSCs transplanted on membranes or with supportive matrix.

Confocal images of sections of the seminiferous tubules illustrated the existence of various iPS cell - derived EGFP - positive cells, including flattened fibroblast cell - like myoid at the outermost layer of the tubules, spermatogonial stem cells with spherical nuclei closest to the basement membrane and spermatocytes with distinctive chromatin closer to the lumen than spermatogonial stem cells (Figure 6D).

JOE PALCA: Well, John, what they did was they - instead of starting with embryos and using those to derive embryonic stem cells, they started with skin cells, or actually precursors to skin cells called fibroblast, that we all have in our skin.

Since murine NSCs have been «primed» with several of the factors originally discovered to reprogram fibroblasts into iPS cells, they represent an attractive source of starting material for iPS cell induction studies.

Normal human lung fibroblasts (NHLF) cells grown in 384 - well Optilux plates were treated with candidate compounds and incubated for 18 hours prior to fixation and staining with Hoescht dye and anti-HMOX1 antibody as described in Materials and Methods.

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

Then, by treating the fibroblasts with a unique «cocktail» of molecules and reprogramming factors, they transformed the cells into endoderm - like cells.

While this manuscript was in preparation, a similar episomal strategy was used to reprogram human primary fibroblasts using a distinct pluripotent cocktail of factors, validating our methods, but with significant lower efficiency (only 3 to 6 colonies / 106 input cells)[42].

Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).

The next step proved even more promising: when we introduced these three genes into the injured hearts of living mice, we were again able to convert fibroblasts into new heart cells, and these cells helped improve heart function, integrating with the old ones and beating in synchrony with the rest of the heart.

To make the tissues, the scientists crack open fertilized chicken eggs, pull fibroblasts and muscle cells out of the embryos» hearts, and mix them together with collagen.

Shi, Y.; Desponts, C.; Do, J. T.; Hahm, H. S.; Scholer, H. R.; Ding, S. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small - molecule compounds.

The CMTA provided and coordinated nineteen patients with a number of Charcot - Marie - Tooth diseases who provided fibroblasts, or skin cells, for research.

Furthermore, using a phospho - tyrosine [Y] 474 (in - house generated) and a phospho - serine [S] 176 (Cell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right panCell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right pancell line SW480 (Fig. 3E, right panel).

Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.