Sentences with phrase «final reaction volume»

An average of ∼ 500 ng of total RNA input per reaction was diluted in water to a total volume of 25 µl, and 25 µl of master mix that contained EZ buffer, rTth enzyme, dNTPs, Rox reference dye, MnCl2 and primer / probe was added to obtain a final reaction volume of 50 µl.

Not exact matches

Quantitative PCR was conducted in triplicate in an Applied Biosystems MicroAmp Optical 96 - well Reaction Plate with a 25 µl reaction volume containing 12.5 µl of Thermo Scientific Maxima SYBR Green / ROX qPCR Master Mix, 2 µl of purified DNA and a final primer concentration of 0.15 µM for both forward and reverse Reaction Plate with a 25 µl reaction volume containing 12.5 µl of Thermo Scientific Maxima SYBR Green / ROX qPCR Master Mix, 2 µl of purified DNA and a final primer concentration of 0.15 µM for both forward and reverse reaction volume containing 12.5 µl of Thermo Scientific Maxima SYBR Green / ROX qPCR Master Mix, 2 µl of purified DNA and a final primer concentration of 0.15 µM for both forward and reverse primers.