Oocytes with
a first polar body were enucleated manually in the presence of 7.5 µg / ml of cytochalasin B.
Following in vitro maturation, oocytes at metaphase II with the presence of
a first polar body were activated using 10 µM calcium ionophore A23187 (Sigma - Aldrich) for 5 min followed by 2 mM DMAP (Sigma - Aldrich) for 4 h at 37 °C in 6 % CO2, 5 % O2 and 89 % N2.
His team collected eggs from 11 volunteers using the standard IVF approach, which involves collecting eggs just as
the first polar body forms.
Not exact matches
The sea is just 5 ° north of the Martian equator and would be the
first discovery of a large
body of water beyond the planet's
polar ice caps.
SKYACTIV -
Body and SKYACTIV - Chassis describe the massive reduction of body weight through extensive use of high - tensile strength steel and the industry's first use of 1800MPa in the bumpers (which also reduces polar moment front and re
Body and SKYACTIV - Chassis describe the massive reduction of
body weight through extensive use of high - tensile strength steel and the industry's first use of 1800MPa in the bumpers (which also reduces polar moment front and re
body weight through extensive use of high - tensile strength steel and the industry's
first use of 1800MPa in the bumpers (which also reduces
polar moment front and rear).