Sentences with phrase «fixed cell imaging»
For this we use gene replacement, live and fixed cell imaging, conditional localization and (in) activation approaches, proteomics, biochemistry and structural biology.
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Furthermore, the microscope will be capable of performing live - cell super-resolution imaging through structured illumination microscopy (SIM) and Super-Resolution Radial Fluctuations (SRRF); for fixed cells resolutions on the scale of tens of nanometres will be achievable using single molecule localization microscopy (SMLM) techniques.
By using a combination of genetics, fix and live imaging, cell biology and molecular biology techniques we are exploring the consequences of polyploidy in the brain and epithelial tissues of Drosophila.
HeLa cells were untreated or treated with 1mM Deferoxamine (DFO) for 24h and fixed with paraformaldehyde for imaging by fluorescent microscopy.
(Po) STAC (Polycistronic SunTAg modified CRISPR) enables live - cell and fixed - cell super-resolution imaging of multiple genes.