Furthermore, the microscope will be capable of performing live - cell super-resolution imaging through structured illumination microscopy (SIM) and Super-Resolution Radial Fluctuations (SRRF); for
fixed cells resolutions on the scale of tens of nanometres will be achievable using single molecule localization microscopy (SMLM) techniques.
Not exact matches
«Looking at a
fixed cell at high
resolution can tell you where different parts of the
cell are at any given moment; but because much of biology depends on the movement of very small proteins finding each other and interacting, we really needed to look at how things move in a live
cell.»
We will use high and super-
resolution fluorescence microscopy like total internal reflection fluorescence microscopy (TIRFM) and fluorescence photo - activation localization microscopy (FPALM) to visualize and track the spatio - temporal dynamics of tethering and SNARE proteins in live and
fixed cells with single molecule
resolution.
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