Sentences with phrase «flanked exon»

Primers flanking exons 14, 15 and the activation loop region of exon 20 of the FLT3 gene, and exon 12 of the NPM1 gene are used to amplify DNA extracted from a patient sample.

Not exact matches

From these efforts, we identified a high - quality orthologous gene set across avian species, consisting of exons from 8251 syntenic protein - coding genes (~ 40 % of the proteome), introns from 2516 of these genes, and a nonoverlapping set of 3769 ultraconserved elements (UCEs) with ~ 1000 bp of flanking sequences.

We amplified cDNA from wild - type and mutant ovaries (and genomic DNA as a control) using primers located in the exons flanking the mutation.

cDNA was amplified using Taq DNA Polymerase (Invitrogen) using exon - flanking and intron - spanning primers.

The mutation is a 44 bp insertion of a A29 tract flanked by a 15 bp duplication in exon 2 of the gene, that creates a frameshift and introduces a premature stop codon early in exon 3.