Sentences with phrase «flourochrome labeled antibody»

«In contrast, in age - matched fetuses from germ - free mothers, these labeled antibodies easily crossed the blood - brain barrier and was detected within the brain parenchyma.»

«We showed that the presence of the maternal gut microbiota during late pregnancy blocked the passage of labeled antibodies from the circulation into the brain parenchyma of the growing fetus,» says first author Dr. Viorica Braniste at the Department of Microbiology, Tumor and Cell Biology at Karolinska Institutet.

Wellington and her colleagues detected the dormant cells with fluorescent - labelled antibodies which cling specifically to S. typhimurium.

Labeled antibodies, which bind to specific cell types, allowed the researchers to determine which types of neuron were being created at which times.

Using colorfully labeled antibodies to light up the new protein under their microscopes, they found that it was present where Listeria entered cells and also present at adherens junctions between epithelial cells.

Target bacteria were captured using antibody - coated magnetic beads and then encapsulated in picoliter - sized droplets with fluorescently labeled antibodies for detection.

To determine pluripotency and AcGFP1 knockout efficiency in gesicle - treated cells, cells were labeled with a fluorescently labeled antibody specific to the pluripotency marker SSEA - 4.

Oligonucleotide - labeled antibodies allow integration of cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed protein markers far surpassing what can be measured by cytometry - based approaches.

We make use of fluorescently - labeled antibodies and FACS machines to sort specific cellular populations.

Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using fluorescently labeled antibodies.

After the sample and buffer are added to the sample well any antigens in the sample migrate across a nitrocellulose membrane along with the gold - labeled antibody (conjugate) and form an antigen / antibody gold complex.

Excess colloidal gold - labeled antibody migrates across to the control line, where an antibody directed to the gold - labeled antibody binds to it.

The Cartridge uses lateral flow assay technology and fluorescence - labelled antibodies to selectively detect specific drugs or their metabolites in eccrine sweat collected from a fingerprint.

Key Highlight: • Handled flourochrome labeled antibody staining used for flowcytometry, cell proliferation assay, immunofluorescence cell staining and cytokine assays using ELISA.

Not exact matches

They also utilized immunogold labeling — in which gold particles are attached to antibodies that bind to particular proteins in order to make them visible in electron microscopy — to show that filaments within the feathers were keratin.

Generic labeling of proteins is achieved through fluorescent - antibody binding.

A radio - labeled human antibody binds to the viral gp41 protein expressed on the surface of the HIV - infected human lymphocyte and the cell is killed by alpha radiation delivered by the antibody.

The researchers selected one of the antibodies, labeled 3B11, as a «lead» candidate for further research.

Diego Grassi, PhD, a research associate in Lasmézas» lab, made this discovery by labeling the pα - syn * with an antibody so he could follow it throughout the cell after it was created.

Typically, detection of biomolecules such as proteins are performed using colorimetric assays or fluorescent labelling with a secondary antibody for detection, and requires complex optical detection equipment such as fluorescent microscopy or spectrophotometry.

In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the technique works on tissue that has been previously labeled with either fluorescent antibodies or proteins such as green fluorescent protein (GFP).

Brilliant Violet dye conjugated secondary antibodies from Jackson ImmunoResearch allow the addition of more colors to multiple labeling assays.

BV421 and BV480 are available conjugated to a range of secondary antibodies recommended for multiple labeling due to their minimal cross-reactivity.

Using an antibody that binds and labels a specific viral protein, the researchers tracked the virus as it first infected nerves in the gut 2 or 3 days after the nasal spray and then successively appeared in the brain stem and midbrain and ultimately infected much of the rest of the brain within 10 days.

In the current study, the researchers used high - affinity antibodies to «label» the cannabinoid receptors so they could be seen using various microscopy techniques, including electron microscopy, which allowed very detailed visualization at individual synapses, or gaps between nerve cells.

Immunoreactions were developed using a fluorescently labeled secondary antibody, and samples visualized in a Leica Biosystems epifluorescent microscope or using a secondary antibody linked to horseradish peroxidase and developed with a 3,3 ′ - diaminobenzidine kit.

The SVCs were incubated with a fluorescently labeled anti-F4 / 80 antibody and were sorted by FACS into F4 / 80 - expressing (F4 / 80 +) and - nonexpressing (F4 / 80 ---RRB- populations.

Cells treated with hamster anti-αβTCR or hamster murine γδTCR were washed three times in HBSS and incubated in PE - labeled Armenian hamster antihamster IgG (PharMingen) secondary antibody for 20 min at 4 °C, washed three additional times in PBS, fixed in 1 % paraformaldehyde, and assessed for fluorescence in a FACScan flow cytometer (Becton Dickinson).

(E) R5 antibody labels Müller glia (Mü; red), which extend processes the breadth of the flank retina.

Difficult - To - Express Proteins: Resolving Bioprocessing Challenges with a Scalable Perfusion Bioreactor Scott Waginer, Emily Wozniak, and Kyle Biesecker (The Cell Culture Company) Among some other recombinant glycoproteins, bi - and tri-specific antibodies are labeled as «difficult - to - express» proteins because of low titers and sensitivity to environmental conditions.

Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes.

Immunofluorescent analysis of HepG2 cells using 60008 -1-Ig (beta actin Antibody) at dilution of 1:50 and Rhodamine - labeled goat anti-mouse IgG (red).

Furthermore, once these proteins are identified, they can be selectively labeled with antibodies linked to green fluorescent protein (GFP) and directly observed in a cell.

In Figure 4, Panel B, cells were incubated simultaneously with anti ‑ SSEA ‑ 4 and anti - TRA ‑ 1 ‑ 60 antibodies, since they were labeled with different fluorophores (PE and FITC, respectively).

ImmunoStar's 5 - HT6R Antibody demonstrates significant labeling of rat cortex, amygdala and hippocampus and other areas using immunohistochemistry and immunofluorescence.

RNA spots are labelled with red dye, nuclei with blue hoechst and the RELA / p65 protein with GFB bound antibodies dye.

Control and gesicle - treated hiPS cells were assessed for AcGFP1 expression (green), labeled with an antibody specific to pluripotency marker Oct ‑ 4, visualized with a fluorescent - labeled secondary antibody (red), and nuclear - labeled with DAPI (blue).

Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004 -1-Ig (GAPDH antibody) at dilution of 1:50 and Rhodamine - labeled goat anti-mouse IgG (red).

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band.

Fab fragments can be used to block endogenous immunoglobulins on cells, tissue, or other surfaces, and to block the exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species.

Many popular methods, such as antibody labeling, are used to add physical fluorescent labels to specific cellular constituents.

Generation, purification, and testing of primary antibodies Gold -, enzyme - and fluorescent labeling of secondary antibodies In situ hybridization Light - and electron microscopy

At 13 months of age, the intracellular MOAB - 2 immunolabel was hardly detectable in pyramidal neurons adjacent to amyloid plaques, the latter which were strongly labeled by this antibody and not by pab27576 (Additional file3: Figure S2 a-b).

Background may derive from sticky soluble immune complexes or from contaminating bovine IgG sticking non-specifically and attracting cross-reacting labeled secondary antibodies.

PromoKine offers a wide range of well proven products for cell biology research such as kits and reagents for cell analysis, apoptosis research, cell transfection, fluorescent labeling, gene cloning & expression, mycoplasma detection and elimination as well as numerous antibodies, ELISAs, cytokines and growth factors.

DDX3 was labeled with specific antibody (red stain) and nuclei were counterstained with hematoxylin (blue color).

For double labeling, the cells were incubated with primary antibodies against TuJ1 and L1 or GFAP, then biotinylated anti-mouse or rabbit (rat) IgG (Vector, Burlingame, CA, USA), and finally with avidin - rhodamine (Vector) and corresponding FITC - conjugated secondary antibodies (from different host species, Chemicon and Molecular Probes, Invitrogen).

Secondary antibody (Affinity Purified Antibody Peroxidase Labeled Goat Anti-Human IgG (γ), KPL, Gaithersburg, Maryland, United States: Cat # 074 - 1002) at a 1 ∶ 4,000 dilution, was added and incubated for 1 hour at 22 °C, after which substrate (ABTS Peroxidase Substrate System (2 - Component), KPL: Cat # 50-62-01) was added and incubated for an additional hour aantibody (Affinity Purified Antibody Peroxidase Labeled Goat Anti-Human IgG (γ), KPL, Gaithersburg, Maryland, United States: Cat # 074 - 1002) at a 1 ∶ 4,000 dilution, was added and incubated for 1 hour at 22 °C, after which substrate (ABTS Peroxidase Substrate System (2 - Component), KPL: Cat # 50-62-01) was added and incubated for an additional hour aAntibody Peroxidase Labeled Goat Anti-Human IgG (γ), KPL, Gaithersburg, Maryland, United States: Cat # 074 - 1002) at a 1 ∶ 4,000 dilution, was added and incubated for 1 hour at 22 °C, after which substrate (ABTS Peroxidase Substrate System (2 - Component), KPL: Cat # 50-62-01) was added and incubated for an additional hour at 22 °C.

Frozen sections of affected tissues were then stained with fluorescently - labeled Borrelia - specific polyclonal antibodies.

(E) Dilp5 in the IPCs was detected by anti-Dilp5 antibody in wild - type and CCHa2 - R mutants, in which the IPCs were labeled by dilp2 - GAL4 driving UAS - mCD8:: GFP.