After gesicle treatment, assessment of CD81 expression by
flow cytometry identified that 57.8 % of gesicle - treated hiPS cells were CD81 negative (Figure 4, Panel A).
Not exact matches
To overcome these limitations, we used two - color
flow cytometry to
identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).
Identify immune cell populations with our
flow cytometry antibodies and kits and isolate them with our cell selection kits.
In total, 106 splenocytes were cultured without peptide or with 0.2 μM LCMV gp33 peptide, NP396 peptide for 5 h. IFN - γ positivity by
flow cytometry was used to
identify LCMV - specific cells (13).
Conventional
flow cytometry is a powerful technique for measuring cell phenotype and function, but it relies on fluorescent stains, or labels, to
identify particular cell subpopulations.