Sentences with phrase «flow cytometry in»

At these time points, mixed chimerism was monitored by flow cytometry in venous blood, spleen and bone marrow.

(B) The expression of MHC antigens by the chimeric hematopoietic cells was determined by flow cytometry in the Rag2 − / − γc − / − mice.

Expression of Venus and SSEA - 1 were quantitated by flow cytometry in two independent clonal lines and compared to both control and parental cells.

Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes.

A new joint International Dairy Federation (IDF) / International Organization for Standardization (ISO) Standard (ISO 19344 IDF 232) has just been released to provide a method for the quantification of lactic acid bacteria by flow cytometry in fermented products, starter cultures, and probiotics used in dairy products...

Not exact matches

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

CST has about 225 employees, and specializes in making antibodies to be used in research applications such as immunohistochemistry and flow cytometry.

Also covered will be techniques for analyzing exosomes, microvesicles, and apoptotic bodies in unprocessed samples, how imaging flow cytometry can be used to evaluate or reevaluate EV isolation techniques, and the advantages and disadvantages of using this method.

Imaging flow cytometry (IFC) has emerged as a useful and efficient tool for studying the signaling pathways in immunophenotypically defined subpopulations of immune cells.

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

Secondary antibodies play an important role in many research applications, including immunofluorescence, immunohistochemistry, Western blot, ELISA, flow cytometry, and more.

Using flow cytometry, in which fluorescent probes are used to detect IgA - coated bacterial species, the researchers discovered that IgA - coated E. coli were abundant in fecal samples from patients with both Crohn's disease and spondyloarthritis.

The two plots in the Nature paper show flow cytometry data - a counting up of cells - after exposure to other cells in bone marrow taken from younger and older mice.

Analytical flow cytometry analysis was performed on whole blood after lysis of erythrocytes and fixing in 2 % PFA.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

IDMIT will contribute 1) To the development and validation of assays based on flow cytometry and mass cytometry for the evaluation of immune responses in humans and animal models; these tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal model platform, in particularly by providing access to NHP models and to new technologies for in vivo imaging infections and host responses; 3) To networking activities, in particular by organising a workshop on in vivo imaging.

The immune responses will be evaluated in the different species by measuring influenza antigen - specific IgG / IgA and IgM, neutralising antibodies and antibodies inhibiting hemagglutination and antigen specific lymphocyte responses and cytokine secretion profiles by advanced flow cytometry.

IDMIT is an infrastructure for preclinical research in infectious diseases and immunology which is certified ISO9001 and which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology, flow cytometry and mass cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).

Dr. Ruxandra Sîrbulescu analyzes flow cytometry data on immune cells used in a wound healing study.

The team has access to state - of - the - art laboratories and animal facilities with BSL2 and BSL3 containment, flow cytometry, cell sorting, histopathology and in vivo imaging (IVIS).

Moreover, the allospecific apoptotic activity of the putative DN T cells was abolished in two separate experiments using in vitro treatment with anti-Thy 1.2 antibody plus complement, which removed > 97 % of the Thy 1 + cells, as determined by flow cytometry.

Works well in flow cytometry and immunohistochemistry.

The department has expertise and infrastructure to support bacterial and viral analysis, and the detailed evaluation of cellular and serological immune responses including flow cytometry and cell sorting, Luminex multiplex platforms, real time PCR, microarrays, comprehensive histological staining, immunohistochemistry and in - situ hybridisation.

Quick and reliable assessment of chronological life span in yeast cell populations by flow cytometry.

The flow cytometry facility at the Wellcome Sanger Institute is a dedicated scientific service offering investigators with state of art flow cytometry instrumentation together with assistance in running samples, data analysis and experimental design.

Someone who knows a thing or two about the importance of data collection is Michael Andreeff, M.D., Ph.D., professor of Leukemia, who was a pioneer in the develop of flow cytometry, a method for counting and sorting cells that is commonly used in today's clinical trials.

(d) Histogram of membrane - bound Tie2 expression in HUVEC and BP compared to isotype control measured by flow cytometry.

All of our team members have many years of experience in different aspects of flow cytometry.

Surface expression of Tie2 in BP and HUVEC as well as isolated mBP was analysed by flow cytometry.

In accordance with the flow cytometry data, expression of AcGFP1 in gesicle - treated cells was knocked out in the majority of cells, despite AcGFP1 being expressed in nearly all control cellIn accordance with the flow cytometry data, expression of AcGFP1 in gesicle - treated cells was knocked out in the majority of cells, despite AcGFP1 being expressed in nearly all control cellin gesicle - treated cells was knocked out in the majority of cells, despite AcGFP1 being expressed in nearly all control cellin the majority of cells, despite AcGFP1 being expressed in nearly all control cellin nearly all control cells.

In total, 106 splenocytes were cultured without peptide or with 0.2 μM LCMV gp33 peptide, NP396 peptide for 5 h. IFN - γ positivity by flow cytometry was used to identify LCMV - specific cells (13).

These finding were exactly in accordance with Gillet et al., 1996 [24], who stated that, Cyclin D1 staining did not appear to correlate with an increased S - phase fraction as judged by flow cytometry.

The percentage of CSCs in the cycling hypoxia selected subpopulation was analyzed based on the CD44, CD24, ESA, and E-cadherin expression by three - color flow cytometry.

Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.

No significant correlation was observed between Cyclin D1 staining and S - phase % obtained by flow cytometry as illustrated in Table 5.

By supporting the development of innovative, cutting - edge applications in the areas of super-resolution microscopy, flow cytometry, Raman spectroscopy and metrology, the Cobolt lasers can contribute to advancing the understanding and defeating of severe diseases, to improvements in quality of life and a better environment.

Amsbio has announces MagSi - Direct - a new technology that brings the power, simplicity and convenience of magnetic separation to researchers involved in cell biology, protein chemistry, flow cytometry, diagnostics development and many other fields.

GFP + leukemia cells in peripheral blood were counted by flow cytometry on day 7 (C) and day 14 (D).

Her current work utilizes flow cytometry to study peptide recognition in the context of the HIV - 1 immune response.

His team applied, among others, techniques of confocal microscopy and cell sorting by flow cytometry which led to the discovery in human muscle biopsies that these myoendothelial cells are located adjacent to the walls of blood vessels.

(B) MNK - 3 cells were treated or not with lethal toxin for 3 hrs and then were simulated with no cytokine, IL - 23 or IL - 1β for 5 hrs in the presence of brefeldin A. Cells were then intracellularly cytokine stained for IL - 22 and analyzed by flow cytometry.

Using clinical samples from a long standing cohort in Thailand we would like to evaluate NK cell activation using multi-parametric flow cytometry.

Using two different viability exclusion dyes via flow cytometry analysis, we observed no difference in the viability of ILCs treated with or without lethal toxin and / or IL - 23 (Fig 1D, 1E and 1F and S1A and S1B Fig).

The Gnoto / Axenic Facility of the Instituto Gulbenkian de Ciência hosted clients in previous user projects to perform on site e.g. histology, flow - cytometry or microscopy.

PHENOMIN - CIPHE, on the strength of its expertise in genetics and mouse immunology, is positioned as a pioneer in multiparametric analysis of the immune system of mutant mice in normal conditions or exposed to risks of infection, by an integration of innovative research and technical knowledge in the field of flow and mass cytometry as well as advanced microscopy.

In vitro expanded ILCs were stimulated with IL - 1β, IL - 23, PMA, ionomycin or a combination of these stimuli for 5 hr in presence of brefeldin A. Cells were analyzed by ICS and flow cytometry for IL - 22 and GM - CSIn vitro expanded ILCs were stimulated with IL - 1β, IL - 23, PMA, ionomycin or a combination of these stimuli for 5 hr in presence of brefeldin A. Cells were analyzed by ICS and flow cytometry for IL - 22 and GM - CSin presence of brefeldin A. Cells were analyzed by ICS and flow cytometry for IL - 22 and GM - CSF.

Nanog overexpressing HV cells were grown in the presence of LIF and the fraction of these cultures that expressed the amplified Venus transgene quantitated by flow cytometry.

Cells grown as in (A) were subject to flow cytometry.

A diverse portfolio of high - quality primary and secondary antibodies is available for use in cancer research, epigenetics, immunology, neuroscience, and stem cell research as well as validated for multiple applications, including flow cytometry, western blotting, and immunoprecipitation.

This novel assay allows the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell surface and intracellular proteins using standard flow cytometry.