Single cell Q - RTPCR for the genes indicated was carried out on cells separated by
flow cytometry on the basis of cell surface marker expression.
GFP + leukemia cells in peripheral blood were counted by
flow cytometry on day 7 (C) and day 14 (D).
Not exact matches
The centre will be structured around six technical platforms focusing
on zebrafish transgenesis and micromanipulation, bioimaging, mouse transgenesis,
flow cytometry, stem cell culture, and gene - expression analysis.
Her current research focuses
on developing clinical applications for imaging
flow cytometry, with special attention to transcription factor pathways.
He has worked in the biotech industry as a research scientist for over 11 years with a focus
on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing
flow cytometry reagent kits to speed up assay development time for researchers.
They have screened combinations of antibodies that bind to specific proteins
on the surface of the immature and mature stem cells and that can be used for
flow cytometry, a common laboratory technique for sorting cells.
«We are working
on similar principles,» he says, using particle image
flow cytometry and high - speed cameras to try to reverse engineer a theoretical model.
«If you get cleavage, you'll lose the green tag,» said Cameron Smurthwaite, manager of SDSU's
flow cytometry core facility and lead author
on the Journal of Visualized Experiments paper.
Analytical
flow cytometry analysis was performed
on whole blood after lysis of erythrocytes and fixing in 2 % PFA.
IDMIT will contribute 1) To the development and validation of assays based
on flow cytometry and mass
cytometry for the evaluation of immune responses in humans and animal models; these tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal model platform, in particularly by providing access to NHP models and to new technologies for in vivo imaging infections and host responses; 3) To networking activities, in particular by organising a workshop
on in vivo imaging.
Dr. Ruxandra Sîrbulescu analyzes
flow cytometry data
on immune cells used in a wound healing study.
Peter is also heavily involved with teaching microscopy and
flow cytometry which includes organising and teaching on both the RMS Light Microscopy Summer School and the RMS Practical Flow Cytometry
cytometry which includes organising and teaching
on both the RMS Light Microscopy Summer School and the RMS Practical
Flow CytometryCytometry courses.
Our Luminex 100 and 200 systems are based
on the principles of
flow cytometry.
All
flow cytometry data were acquired
on an LSRII cytometer (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (Tree Star, Ashland, OR).
The expression of CCR5
on their surface was analyzed by laser
flow cytometry.
The Institute is currently running
on a Site License by Flowjo, a
flow cytometry data analysis software package which offers a range of analysis platforms that includes compensation, cell cycle, proliferation studies and kinetics.
Experts and Key Opinion Leaders
on the topic will share their experiences and views
on potency assays and cell characterization by
flow cytometry.
The percentage of CSCs in the cycling hypoxia selected subpopulation was analyzed based
on the CD44, CD24, ESA, and E-cadherin expression by three - color
flow cytometry.
• Flowmetric — Founded by CEO, Ren Capocasale, Flowmetric is a contract research organization focused
on providing polychromatic
flow cytometry and cell sorting services to bio-pharma and drug discovery companies.
Conventional
flow cytometry is a powerful technique for measuring cell phenotype and function, but it relies
on fluorescent stains, or labels, to identify particular cell subpopulations.
The Gnoto / Axenic Facility of the Instituto Gulbenkian de Ciência hosted clients in previous user projects to perform
on site e.g. histology,
flow -
cytometry or microscopy.
PHENOMIN - CIPHE,
on the strength of its expertise in genetics and mouse immunology, is positioned as a pioneer in multiparametric analysis of the immune system of mutant mice in normal conditions or exposed to risks of infection, by an integration of innovative research and technical knowledge in the field of
flow and mass
cytometry as well as advanced microscopy.
Practical training
on multi-parameter
flow, mass and spectral
cytometry (respectively MFC, MMC and MSC) will be performed
on one single site at CIPHE (Marseille).
The purpose of the
flow cytometry core facility is to sort cells for users, and to train facility users
on the self - service cytometers and related software for data acquisition and analysis.
HV ES cells grown under self - renewing conditions were fractionated by
flow cytometry into four fractions based
on Venus (V) and SSEA - 1 (S) expression.