His team applied, among others, techniques of confocal microscopy and cell sorting by
flow cytometry which led to the discovery in human muscle biopsies that these myoendothelial cells are located adjacent to the walls of blood vessels.
For confirming our study results, the correlation between Cyclin D1 - staining scores and the percentage of S - phase obtained by
flow cytometry which showed no significant correlation between Cyclin D1 protein and increased S - phase %.
Peter is also heavily involved with teaching microscopy and
flow cytometry which includes organising and teaching on both the RMS Light Microscopy Summer School and the RMS Practical Flow Cytometry courses.
Not exact matches
Using
flow cytometry, in
which fluorescent probes are used to detect IgA - coated bacterial species, the researchers discovered that IgA - coated E. coli were abundant in fecal samples from patients with both Crohn's disease and spondyloarthritis.
Cells were then analyzed by
flow cytometry after being stained simultaneously with anti-CXCR4 clone 4G10,
which recognizes the N - terminus, and clone 12G5 whose epitope includes the second extracellular loop that is disrupted by the X4 - ZFNs.
IDMIT is an infrastructure for preclinical research in infectious diseases and immunology
which is certified ISO9001 and
which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology,
flow cytometry and mass
cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).
Moreover, the allospecific apoptotic activity of the putative DN T cells was abolished in two separate experiments using in vitro treatment with anti-Thy 1.2 antibody plus complement,
which removed > 97 % of the Thy 1 + cells, as determined by
flow cytometry.
Mass
cytometry, or CyTOF, is a variation of
flow cytometry in
which antibodies are labeled with heavy metal ion tags rather than fluorochromes.
Peter O'Toole heads the Imaging and
Cytometry Labs within the Technology Facility at the University of York
which includes an array of confocal microscopes,
flow cytometers and electron microscopes.
The Institute is currently running on a Site License by Flowjo, a
flow cytometry data analysis software package
which offers a range of analysis platforms that includes compensation, cell cycle, proliferation studies and kinetics.
Figure S1 shows QRT - PCR data for expression of the TGF - beta superfamily member GDF3, a gene
which is strongly downregulated across the various subpopulations, for four ES cell lines subjected to separation by
flow cytometry.
It's important to classify a patient with lymphoma classified into T or B cell,
which can be done easily by a fine needle aspiration cytology
flow cytometry at Colorado.