Sentences with word «floxed»

The progranulin - insufficient mice used for this study were bred from a line generated by crossing Grn - floxed mice with mice carrying β - actin Cre, which were subsequently back - crossed onto a C57BL / 6J background (Martens et al., 2012; Filiano et al., 2013).

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Another problematic scenario is the use of inducible and conditional systems involving lines with different backgrounds (e.g., cre - expressing lines crossed with floxed alleles).

Inducible LPL — deleted mice were created by crossing floxed Lpl (Lplfl / fl) mice with β - actin — driven tamoxifen - inducible Cre (Mer / Cre / Mer) transgenic mice to obtain the Lplfl / fl Actb3 - MerCreMer + offspring designated as iLpldf mice, as previously described (22).

These Cre - off switches may be referred to as DO (Double - floxed Orientation) and could be used to study the physiology or behavior of certain cell populations.

Sometimes also referred to as DiO (Double - floxed inverse Orientation), FLEx systems are generally considered «Cre - On» meaning that your gene of interest starts in the inverse / antisense «off» position and is flipped to the sense (on) orientation in the presence of Cre.

SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr - unc - 119) for easy screening of the insertion event, and a variety of connector modules (linker sequences between the tag and homology arms).

Is tart cherry juice safe for reducing inflammation / fever after floxing?

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Lpar2 - / - mice and Enpp2 - flox mice were provided by Dr. Jerold Chun (the Scripps Research Institute)(Contos et al., 2002) and Dr. Woulter H. Moolenaar (The Netherlands Cancer Institute)(van Meeteren et al., 2006), respectively, to one of us (JA).

I will be on a panel with my friends, AV Flox, Evan Marc Katz, Liz H. Kelly, and Linda Sherman.

wt and autophagy - deficient Atg7flox / flox; Nes - Cre primary neurons expressing APP were stained for Aβ.

These vectors are very similar, both with polylinkers for the addition of gene - specific 1 kb homology arms flanking a FLOXed neoR gene.

Then, if necessary for subsequent experiments, the floxed neoR gene is removed via infection with adeno - cre.

Then, if necessary for subsequent experiments, the floxed neo

These vectors are very similar, both with polylinkers for the addition of gene - specific 1 kb homology arms flanking a FLOXed neo

During this webinar, Dr. Haoyi Wang, one of the developers of this revolutionary technology will share how CRISPR / Cas has been used successfully in mice to generate endogenous knock - in alleles, conditional («floxed») mutations, as well as multiple mutations in a single generation.

The floxed neo cassette contains a strong tpA stop sequence which prevents the transcription of EYFP.

A construct containing a floxed PGK - neo cassette and an enhanced yellow fluorescent protein (EYFP) gene was inserted into the Gt (ROSA) 26Sor locus via homologous recombination.

The lab has generated «floxed» alleles of GABAA receptor subunit genes that allow us to ablate the respective subunit in a subregion specific manner — e.g., in hippocampal subregions or the amygdala — either via a cross with cre - transgenic mice or via stereotaxic injection of cre - expressing viruses into defined brain regions.

In some cases affected transcripts in Cre +; Tsc1 flox / flox mice showed significant variation among individuals that can not simply be explained by differential effectiveness of CRE - mediated knockout since, except one animal, all Cre +; Tsc1 flox / flox mice showed similarly reduced expression levels of Tsc1 mRNA.

After 20 rounds of preparative cell sorts, the mutational machinery was stopped by inducing the excision of the floxed AID gene which is necessary for SHM [10].

However, leaky background activity of MerCreMer can lead to undesired excision of the floxed AID - GPT expression cassette during prolonged culture.

Establishment of Tsc1flox / flox - GFAP - Cre (Tsc1GFAP CKO) mice for evaluating potential new antiepileptic therapies for tuberous sclerosis complex S. C. LEISER, A GHAVAMI, M KWAN, J BELTRAN, D SONG, D. M. DEVILBISS, M WONG, N RENSING, S. L. ROBERDS, D BRUNNER... Abstract / Posters

Expression levels between control (Cre -; Tsc1 + / flox) and Cre +; Tsc1 + / flox mice were similar for all transcripts reported in this study.

AID expression in the DT40 clones (a prerequisite for continuous hypermutation) was assured by transfection of a floxed AID - IRES (internal ribosome entry site)- GPT (guanine phosphoribosyl transferase) bi-cistronic cassette.

To isolate single colony variants in a non-mutating background, the floxed AID expression cassette was excised by culturing the cells in CM containing 20 nM 4 - hydroxitamoxifen (SIGMA) for three days followed by two rounds of cell sorting for stabilization.

The DT40 cell clone used expresses an inducible form of the Cre recombinase (a MerCreMer fusion protein) in order to excise the floxed AID and thereby stop mutational activity if desired.

In contrast, Tsc1GFAPCKO mice (Cre +; Tsc1 flox / flox) showed genotype - dependent changes in some transcripts analyzed.

Mizushima's group found their Atg5flox / flox; CAG - Cre mutant mice displayed a mosaic phenotype for an unknown reason even with the ubiquitous expression of the Cre recombinase [18].

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Other libraries have been created using Starling, including Feathers, Citrus Engine, Dragon Bones, StarlingPunk, Flox, and GameBuilder Studio.