Sentences with phrase «fluorescence microscopy techniques»

The developed microscopy approach combines two fluorescence microscopy techniques: REversible Saturable OpticaL Fluorescence Transitions (RESOLFT) Microscopy with reversible switchable fluorescent proteins (RSFPs) and 4Pi - Microscopy.

The developed microscopy approach combines two fluorescence microscopy techniques: REversible Saturable OpticaL Fluorescence Transitions (RESOLFT) Microscopy and 4Pi Microscopy.

From 2000 to 2003 he was a research scientist at Evotec, Hamburg, developing advanced fluorescence microscopy techniques for high - throughput drug screening.

Scientists at Albert Einstein College of Medicine of Yeshiva University and their international collaborators have developed a novel fluorescence microscopy technique that for the first time shows where and when proteins are produced.

Not exact matches

«We use a technique called scanning X-ray fluorescence microscopy,» study co-author Satoshi Matsuyama says.

«We applied a new technique called X-ray fluorescence microscopy — it looks at elemental composition,» said Marshall.

The technique, called microenvironmental selective plane illumination microscopy (meSPIM), uses exceptionally long, thin beams of laser light to trigger fluorescence in a sample, causing it to glow.

The technique, called superresolution single - molecule fluorescence microscopy, recently helped scientists at the University of Manchester in England track natural killer (NK) cells, which help destroy cancer and viruses.

Acquiring images using modern techniques such as light sheet fluorescence, confocal, or electron microscopy creates a significant data stream.

This work, with the assistance of soil scientists at the University of KwaZulu - Natal, has involved a suite of techniques, including x-ray fluorescence (to provide quantitative data on minor and trace element composition), x-ray diffraction (to reveal crystal structure and parent rock types of paint ingredients), and environmental scanning electron microscopy (to yield qualitative data on elements present).

And its ability to show the shape of dendrites and trace neuronal processes provides contextual information unavailable through imaging techniques such as electron microscopy, fluorescence microscopy, and magnetic resonance imaging (MRI).

This new technique was made possible by the development in recent years of single - molecule microscopy, which enables scientists to peer into the nano - world by turning the fluorescence of individual molecules on and off.

A common technique is fluorescence microscopy, where fluorescent molecules («probes») are attached to cell structures and then «lit up» against a dark background.

By developing a new fluorescence microscopy - based technique, the researchers were able to measure how long it takes proteins to move over distances ranging from 0.2 to 3 micrometres in living cells.

Evidence of the nanoparticles in tumor tissue was found using fluorescence microscopy, a technique capable of detecting the chemotherapeutic drug (camptothecin) attached to the nanoparticle.

A number of imaging techniques will be applied, like fluorescence microscopy and single - molecule AFM.

Optical imaging and spectroscopy, advanced optical imaging techniques in particular super-resolution fluorescence microscopy, optical physics, nanotechnology, single - molecule biophysics and macromolecular biochemistry, single - molecule imaging, gene expression, computational modeling, single - molecule (force) spectroscopy, biomolecular engineering, nucleic acid nanotechnology

Colocalization analysis in fluorescence microscopy Cell Imaging Techniques (pp. 97 - 109): Springer.

Fluorescence correlation spectroscopy (FCS) in microscopy kinetic measurements, principles, analysis and its relation to other photo - kinetic techniques

Michael Berndt (Diez, MPG)-- «Optical Near - fields in fluorescence microscopy - Techniques to access information in the third dimension on the nanometer scale» (2010)

We will continue to study the relationship between the 3D structure and the function of macromolecular complexes using a combination of 3D Electron Microscopy, Cryo - Electron Tomography, single particle analysis, correlative fluorescence microscopy, image analysis, and needed biochemical techniques.

His group focuses on novel techniques and instrumentation for high - resolution structural and molecular microscopy based on integrated fluorescence and electron microscopy.

We work across disciplines and use a variety of techniques including microfluidics, standard microscopies (electron, optical, fluorescence, confocal), spectroscopies (fluorescence, UV, CD), scattering techniques (X-ray, light), protein expression and characterization and cell - free gene expression to investigate the utility of coacervate microdroplets as robust reaction compartments and cellular mimics.

Because electron microscopy requires objects to be dried and flattened, the researchers used a fluorescence - based imaging technique called â $ œDNA PAINTâ $ to visualize the jungle - gym - like structures in solution.

Correlative light and electron microscopy (CLEM) is an imaging technique that combines electron microscopy (EM) with fluorescence localization data.

The techniques most widely used in this industry are X-ray diffraction, X-ray fluorescence microscopy, X-ray Absorption Spectroscopy and X-ray powder diffraction.

Due to its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern biology (1).

«We applied a new technique called X-ray fluorescence microscopy — it looks at elemental composition,» confirmed Marshall.

The projects are multidisciplinary, involving a variety of techniques including molecular biology (PCR, cloning), cell culture and advanced fluorescence microscopy.

In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridization.

Proficient with atomic force microscopy, fluorescence spectroscopy, protein purification and characterization techniques.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign