The data collected by microarray are based on
the fluorescence signal intensities (analogue data) from known sequenced oligo probes.
Photobleaching is a photochemical alteration process that decreases
the fluorescence signal intensity.
Not exact matches
Percent ROS or glutathione was calculated using
fluorescence intensity; a 3 - sigma increase in
signal over control (solvent only) was deemed positive.
It allows to measure
fluorescence levels in closely associated sperm tails, through an exact distinction between positive and background
signals, and provides background - corrected pixel
intensity values that can directly be used for data analysis.
Fluorescence intensity was quantified, normalized to background and DRAQ5
signal expressed as percentage of control.