Sentences with phrase «fluorescent cell imaging»

The lab tracked the differentiating process using fluorescent cell imaging, which tested for the necessary markers during each step.

Not exact matches

Huerta also calls attention to fluorescent resonance energy transfer, which he says, «really allowed us to start imaging processes in cells as they occur.»

«The massive advantage with our stain-less laser - based imaging approaches is that you can use the stem cell sample without having to interrupt the developmental process in real time, you don't need to perform any cell disruption and there is no photobleaching (fading) which is fairly common with fluorescent material,» Catarina enthused.

To surmount this hurdle, Dr. Hodgson and his colleagues in the Gruss Lipper Biophotonics Center at Einstein devised a new fluorescent protein biosensor that, combined with live - cell imaging, revealed exactly when and where Rac1 is activated inside cancer cells.

These infrared fluorescent proteins are expressed well in mammalian cells and mice, and can be used for whole - body imaging.

Continuous measurement and imaging of the intracellular free calcium ion concentration -LRB-[Ca2 +] i) of mitotic and interphase PtK1 cells was accomplished with the new fluorescent Ca2 + indicator fura - 2.

Through the new imaging technology, researchers use fluorescent imaging to locate proteins and other molecules in cells and tissues.

When the team inserted the new analogues into a living cell and used high - definition, single fluorescent - molecule imaging, they were finally able to directly document the actions of specific gangliosides in a living cell for the first time.

Imaging techniques that rely on light — such as taking pictures of cells tagged with a «reporter gene» that codes for green fluorescent protein — only work in tissue samples removed from the body.

«We hope it will ultimately do for ultrasound what green fluorescent protein has done for light - based imaging techniques, which is to really revolutionize the imaging of cells in ways there were not possible before.»

The group of imaging specialists led by Prof. Michael Schäfers, Coordinator of the Cluster of Excellence, labelled the cells thus obtained with various fluorescent dyes in order to be able to study them in living organisms — initially with the optical method of fluorescence reflectance imaging.

Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology.

Invaluable as markers for monitoring photosynthesis and other energy - related processes in living cells, green fluorescent proteins (GFPs), discovered in a species of jellyfish, are vital in extremely high - resolution imaging studies.

«The high efficiency of the materials along with cheap, scalable synthesis makes them very attractive as next generation emitters for fluorescent lamps, LEDs and for biological imaging, for example for highlighting tumours or cell division.»

Embryonic hemocytes lend themselves beautifully to live imaging studies since fluorescent probes can be expressed specifically in these cells using hemocyte specific promoters and their movements subsequently imaged within living embryos using confocal timelapse microscopy.

They found that injecting into the carotid artery breast cancer cells that express markers allowing them to enter the brain — cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.

The injected cells express markers that allow them to enter the brain and are labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies.

Johanna Spandl (Thiele, MPG)-- «Cell biology of lipid droplets: The function of the lipid droplet protein AUP1 and establishment of the novel fluorescent dye LD540 for improved staining and imaging of lipid droplets» (2009)

Application to Living Cell Fluorescent Imaging.

smFISH can be used to detect specific RNA sequences in a cell based on their binding to a fluorescent probe, but imaging the «dots» corresponding to each RNA molecule requires high magnification and delicate imaging settings.

The 1833 and SCP28 breast cancer cells were engineered to express reporters with luciferase and green fluorescent protein to monitor metastatic progression by bioluminescence imaging (Fig. 2A).

Through clever biochemistry combined with superresolution imaging techniques, Yao and colleagues introduced two EB proteins into cells, one with half of a photoactivatable green fluorescent protein (PAGFP), and one with the other half of PAGFP.

The optical contrast (measured as a ratio of the fluorescent image amplitudes for C4 - 2B to HS - 5) of fluorescent imaging for cancer versus stromal cells was 2.3 (Figure 7B, D).

HeLa cells were untreated or treated with 1mM Deferoxamine (DFO) for 24h and fixed with paraformaldehyde for imaging by fluorescent microscopy.