Sentences with phrase «fluorescent labeled antibodies»

Not exact matches

Generic labeling of proteins is achieved through fluorescent - antibody binding.

Typically, detection of biomolecules such as proteins are performed using colorimetric assays or fluorescent labelling with a secondary antibody for detection, and requires complex optical detection equipment such as fluorescent microscopy or spectrophotometry.

In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the technique works on tissue that has been previously labeled with either fluorescent antibodies or proteins such as green fluorescent protein (GFP).

Wellington and her colleagues detected the dormant cells with fluorescent - labelled antibodies which cling specifically to S. typhimurium.

Furthermore, once these proteins are identified, they can be selectively labeled with antibodies linked to green fluorescent protein (GFP) and directly observed in a cell.

Control and gesicle - treated hiPS cells were assessed for AcGFP1 expression (green), labeled with an antibody specific to pluripotency marker Oct ‑ 4, visualized with a fluorescent - labeled secondary antibody (red), and nuclear - labeled with DAPI (blue).

Many popular methods, such as antibody labeling, are used to add physical fluorescent labels to specific cellular constituents.

Generation, purification, and testing of primary antibodies Gold -, enzyme - and fluorescent labeling of secondary antibodies In situ hybridization Light - and electron microscopy

PromoKine offers a wide range of well proven products for cell biology research such as kits and reagents for cell analysis, apoptosis research, cell transfection, fluorescent labeling, gene cloning & expression, mycoplasma detection and elimination as well as numerous antibodies, ELISAs, cytokines and growth factors.

For colocalization of both tracers or one tracer together with ZENK signals, primary antibodies (Egr - 1 / ZENK, 1 ∶ 500; CtB, 1 ∶ 300 in PBS - T) were detected by an appropriate secondary antibody (polyclonal goat raised against rabbit IgG labeled with fluorescent dyes Alexa488; Molecular Probes Europe BV, Leiden, The Netherlands, 1 ∶ 400 in PBS - T).

Such an optical contrast exceeded the optical contrast of the fluorescent labels (that were targeted to C4 - 2B and HS - 5 cells using the same prostate cancer - specific PSMA antibody, see Figure 7B, D) by 31 times.