Sentences with phrase «fluorescent labeled proteins»

Not exact matches

Blood stem cells in the bone marrow have been labeled with a fluorescent marker protein.

Developmental biologist Francesca Peri of the European Molecular Biology Laboratory in Heidelberg, Germany, and colleagues created genetically engineered versions of the animals that produced microglia labelled with green fluorescent protein, a glowing compound frequently used in laboratory research.

Generic labeling of proteins is achieved through fluorescent - antibody binding.

A microfluidic device captures cells, lyses them, and separates their contents, allowing naturally fluorescent and labeled proteins in a single cell to be counted.

The group employed various viral tracing methods — infecting receptor - expressing neurons with a virus strain and watching them spread as they label infected cells with a fluorescent protein — to visualize the neural circuit downstream of the ESP1 receptor, as well as providing an image of nerve fibers belonging to specific neurons in the brain and synapses relaying impulses from neuron to neuron, to map the anatomical foundation that conveys ESP1 signals in the brain.

Typically, detection of biomolecules such as proteins are performed using colorimetric assays or fluorescent labelling with a secondary antibody for detection, and requires complex optical detection equipment such as fluorescent microscopy or spectrophotometry.

But scientists say that it has potential for even better resolution and for examining protein - protein interactions, particularly if fluorescent labels of different colors can be applied to proteins.

In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the technique works on tissue that has been previously labeled with either fluorescent antibodies or proteins such as green fluorescent protein (GFP).

The new AAV vectors can also deliver genes that code for colorful fluorescent proteins; such proteins are useful in identifying and labeling cells.

Green fluorescent protein labeling allowed them to see the early development pattern and show that lncND, which ordinarily is not present in mice — lncND is present only in some primates including humans — had a functional effect on development.

Smith's team labelled the virus with green fluorescent protein, and labelled some — but not all — cells with a red marker that tagged the actin.

This latter capability is extremely valuable to biomedical research, which relies heavily on fluorescent probes to label and track proteins, nucleic acids, and other cellular components.

To generate an image, all of the fluorescent labels in a protein are switched on, then a wave of light is used to deactivate most of them.

Sandia National Laboratories researchers are drawing inspiration from neurons in the brain, such as these green fluorescent protein - labeled neurons in a mouse neocortex, with the aim of developing neuro - inspired computing systems to reboot computing.

Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual neurons in the brain, to label cells stochastically using gene cassettes that code for four uniquely expressed fluorescent proteins.

By labeling one of the shell proteins with Yellow Fluorescent Protein (YFP), we were able to use confocal fluorescence microscopy to observe punctuate loci forming inside chloroplasts.

The same principle lies behind the powerful and visually spectacular Brainbow cell - labeling technique, in which each labeled cell expresses a unique ratio of three or four distinct colors of fluorescent protein.

The membranes of cells that have been successfully targeted with gesicles are transiently labeled with red fluorescent protein.

One common complaint about using four - color Confetti to label single cells (in an animal) is that the cells express green fluorescent protein less often, or sometimes not at all, compared with cells that express each of the other three colors.

The ability to label different subcellular locations with the green fluorescent protein (GFP) has made it possible to visualize intracellular activities in living cells.

Furthermore, once these proteins are identified, they can be selectively labeled with antibodies linked to green fluorescent protein (GFP) and directly observed in a cell.

Quantitative microscopy of green fluorescent protein - labeled yeast.

In 2002, an offer to join the faculty of MIT's Chemistry Department drew her back to the East Coast, where she was able to begin tackling the problem that had «repeatedly stymied» her throughout her PhD and postdoc years: site - specific protein labeling — how to label a particular protein with a fluorescent tag or other «chemical probe» to reveal information about that protein's function.

For example, with standard fluorescent microscope filter sets, users can label multiple proteins of interest with a combination of green and blue or cyan and yellow moxFPs.

To achieve even cell labeling, they generated farnesylated derivatives of the fluorescent proteins, which are directly trafficked to cell membranes.

Close - up of muscle fibres in a zebrafish with the protein, actin, labelled with green fluorescent protein (GFP) Image credit: Elisabeth Busch, Wellcome Trust Sanger Institute

He used a fluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likfluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likFluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - like activity.

Because the embryo is transparent it is possible to use fluorescent proteins to label specific cells and their components, and then use this to watch the fate of the cells in the presence or absence of different genes.

Neuron expressing a fluorescent protein engineered by the Looger Lab was reconstructed from immunogold labeled serial sections of the mouse brain.

To this end, the two molecules that are putatively interacting are labeled by two different fluorophores or fluorescent proteins.

In early - stage oocytes oskar - TRICK mRNA is labeled by both NLS - PCP - GFP (green) and NLS - MCP - RFP (red), fluorescent RNA - binding proteins, indicating translational repression.

Nerve cells in the mouse brain that have been labeled with a modified rabies virus and fluorescent proteins.

Tags for this Online Resume: Molecular Biology, Protein purification and isolation, Bioinformatics, cloning and sequencing, protein - DNA binding, fluorescent lProtein purification and isolation, Bioinformatics, cloning and sequencing, protein - DNA binding, fluorescent lprotein - DNA binding, fluorescent labeling