Sentences with phrase «fluorescent labeling methods»

However, we recently developed AAV (adeno - associated virus)- based fluorescent labeling methods that can be used with 3DISCO to image non-transgenic animals such as rats (17).

Not exact matches

In order to test the sensitivity and specificity of spectral histopathology, the RUB team compared SHP with classical immunohistochemical methods, in which tumours are identified with the aid of fluorescent labels.

The group employed various viral tracing methods — infecting receptor - expressing neurons with a virus strain and watching them spread as they label infected cells with a fluorescent protein — to visualize the neural circuit downstream of the ESP1 receptor, as well as providing an image of nerve fibers belonging to specific neurons in the brain and synapses relaying impulses from neuron to neuron, to map the anatomical foundation that conveys ESP1 signals in the brain.

The group of imaging specialists led by Prof. Michael Schäfers, Coordinator of the Cluster of Excellence, labelled the cells thus obtained with various fluorescent dyes in order to be able to study them in living organisms — initially with the optical method of fluorescence reflectance imaging.

Instead, the new method, called patterned photoactivation non-linear SIM, begins by switching on just a subset of fluorescent labels in a sample with a pattern of light.

Over time, they developed methods that add fluorescent labels to cells in order to see features the human eye normally can't see.

A general method for site specific fluorescent labeling of recombinant chemokines.

Most colocalization methods are based on pixel overlap between the previously denoised signal that is emitted from two (or more) different fluorescent labels, and use a global image correlation such as Pearson's of Manders» coefficients.

Many popular methods, such as antibody labeling, are used to add physical fluorescent labels to specific cellular constituents.

We develop methods to label RNA in fixed and living cells using fluorescent probes and microscopy techniques and image analysis algorithms to visualize and quantify many mRNAs simultaneously.