The fluorescent labeling technique is the first to allow scientists to identify individual synapses that are active during a complex behavior, such as avoiding heat.
Not exact matches
Traditional
techniques involve
labelling or injecting the cells with stains or fluorophores —
fluorescent compounds that «glow» when exposed to light — to detect their intricate structures.
In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the
technique works on tissue that has been previously
labeled with either
fluorescent antibodies or proteins such as green
fluorescent protein (GFP).
Approach: Wu's group used Brainbow, a transgenic
technique originally devised to map individual neurons in the brain, to
label cells stochastically using gene cassettes that code for four uniquely expressed
fluorescent proteins.
The same principle lies behind the powerful and visually spectacular Brainbow cell -
labeling technique, in which each
labeled cell expresses a unique ratio of three or four distinct colors of
fluorescent protein.
Conventional flow cytometry is a powerful
technique for measuring cell phenotype and function, but it relies on
fluorescent stains, or
labels, to identify particular cell subpopulations.
We develop methods to
label RNA in fixed and living cells using
fluorescent probes and microscopy
techniques and image analysis algorithms to visualize and quantify many mRNAs simultaneously.