Sentences with phrase «fluorescent labels in»

To generate an image, all of the fluorescent labels in a protein are switched on, then a wave of light is used to deactivate most of them.

Instead, the new method, called patterned photoactivation non-linear SIM, begins by switching on just a subset of fluorescent labels in a sample with a pattern of light.

Next, the researchers optogenetically stimulated hippocampal neurons to produce gamma waves in Alzheimer's disease — model mice that had transgenically received both a light - responsive ion channel and a fluorescent label in their hippocampal neurons.

Not exact matches

While light - sheet microscopy is an old idea — scientists at ZEISS Microscopy and collaborators first came up with it in 1903 — only in this century has the convergence of fluorescent labels that work to process image volumes combined to make light - sheet mainstream.

Blood stem cells in the bone marrow have been labeled with a fluorescent marker protein.

Developmental biologist Francesca Peri of the European Molecular Biology Laboratory in Heidelberg, Germany, and colleagues created genetically engineered versions of the animals that produced microglia labelled with green fluorescent protein, a glowing compound frequently used in laboratory research.

A microfluidic device captures cells, lyses them, and separates their contents, allowing naturally fluorescent and labeled proteins in a single cell to be counted.

In order to test the sensitivity and specificity of spectral histopathology, the RUB team compared SHP with classical immunohistochemical methods, in which tumours are identified with the aid of fluorescent labelIn order to test the sensitivity and specificity of spectral histopathology, the RUB team compared SHP with classical immunohistochemical methods, in which tumours are identified with the aid of fluorescent labelin which tumours are identified with the aid of fluorescent labels.

Due to the usage of lateral displacement, the nano - biomolecules can be detected in real - time and the detection is significantly faster in comparison to fluorescent label based detection.

This novel fluorescent label - free approach uses the lateral shifts in the position of the microbead substrate in pillar arrays, for quantifying the biomolecules, based on the change in surface forces and size, without the need of any external equipment.

The group employed various viral tracing methods — infecting receptor - expressing neurons with a virus strain and watching them spread as they label infected cells with a fluorescent protein — to visualize the neural circuit downstream of the ESP1 receptor, as well as providing an image of nerve fibers belonging to specific neurons in the brain and synapses relaying impulses from neuron to neuron, to map the anatomical foundation that conveys ESP1 signals in the brain.

In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the technique works on tissue that has been previously labeled with either fluorescent antibodies or proteins such as green fluorescent protein (GFP).

The new AAV vectors can also deliver genes that code for colorful fluorescent proteins; such proteins are useful in identifying and labeling cells.

Green fluorescent protein labeling allowed them to see the early development pattern and show that lncND, which ordinarily is not present in mice — lncND is present only in some primates including humans — had a functional effect on development.

Biologists at the University of Cambridge used fluorescent labelling to stain cell membranes in skate embryos, and tracked them through the dynamic development process.

To create the labels, the scientists split a fluorescent molecule in half, one half for the talking neuron and one half for the listening neuron.

The fluorescent green, yellow and blue signals enabled the researchers to label different synapses activated by the sensory experience in different colors in the same animal.

The group of imaging specialists led by Prof. Michael Schäfers, Coordinator of the Cluster of Excellence, labelled the cells thus obtained with various fluorescent dyes in order to be able to study them in living organisms — initially with the optical method of fluorescence reflectance imaging.

The most common probes, which use fluorescent markers or radioactive labels to pinpoint the virus's location in a tissue sample, sometimes have difficulty distinguishing the target — HIV RNA and DNA — from surrounding cellular components.

A thalamocortical, or TC neuron labeled with fluorescent dye, as used in Dr. Augustinaite's study.

Sandia National Laboratories researchers are drawing inspiration from neurons in the brain, such as these green fluorescent protein - labeled neurons in a mouse neocortex, with the aim of developing neuro - inspired computing systems to reboot computing.

Specifically, cell cultures from bone tissue were incubated in 10 % DMEM containing 10 ng / ml TNF - α and 10 μg / ml fluorescent probe of acetylated LDL, DiI - Ac - LDL (Biochemical Technologies, Cambridge, MA), for 4 h. Cells were harvested in the manner described above with the exception that during this labeling period, the rat anti-E-selectin mAb (10E9.6) conjugated to FITC replaced PE - conjugated 10E9.6.

Because zebrafish are transparent until their stripes emerge in adulthood, Oehlers could label the bacteria, immune cells, and vasculature with differently colored fluorescent tags and then follow a TB infection over time.

They found that injecting into the carotid artery breast cancer cells that express markers allowing them to enter the brain — cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.

Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual neurons in the brain, to label cells stochastically using gene cassettes that code for four uniquely expressed fluorescent proteins.

We have placed fluorescent labels on portions of the tail regions of Arabidopsis myosin XIs in order to determine whether they localize, and therefore what role a particular myosin may play in movement of different types of organelles.

The same principle lies behind the powerful and visually spectacular Brainbow cell - labeling technique, in which each labeled cell expresses a unique ratio of three or four distinct colors of fluorescent protein.

Over time, they developed methods that add fluorescent labels to cells in order to see features the human eye normally can't see.

One common complaint about using four - color Confetti to label single cells (in an animal) is that the cells express green fluorescent protein less often, or sometimes not at all, compared with cells that express each of the other three colors.

The ability to label different subcellular locations with the green fluorescent protein (GFP) has made it possible to visualize intracellular activities in living cells.

Furthermore, once these proteins are identified, they can be selectively labeled with antibodies linked to green fluorescent protein (GFP) and directly observed in a cell.

The authors then tested the tumor targeting capability of iPSC - EPCs labelled with the near - infrared fluorescent dye DiR in two established mouse breast cancer models.

Briefly, 15 μl of 100 nm diameter rhodamine - labelled fluorescent latex microsphere particles (LumaFluor Corp, Naples, USA) in 200 μl NaCl solution were injected i.v. 24 h before primary tumour removal.

Using fluorescent labels that switch on and off, researchers activate and deactivate fluorescent molecules in specific regions at specific times.

Generation, purification, and testing of primary antibodies Gold -, enzyme - and fluorescent labeling of secondary antibodies In situ hybridization Light - and electron microscopy

Human induced pluripotent stem cell neurons imaged in phase contrast (gray pixels, left)-- currently processed manually with fluorescent labels (color pixels) to make them visible.

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

A cortical layer 2/3 neuron at 21 days in vitro labeled by ex utero electroporation with fluorescent marker for spine visualization.

In 2002, an offer to join the faculty of MIT's Chemistry Department drew her back to the East Coast, where she was able to begin tackling the problem that had «repeatedly stymied» her throughout her PhD and postdoc years: site - specific protein labeling — how to label a particular protein with a fluorescent tag or other «chemical probe» to reveal information about that protein's function.

The fluorescent labeling permitted the authors to monitor tumor outgrowth in vivo after mixtures were injected into either wild - type or Rag2 - knockout (immune system deficient) mice.

Neurosurgeons are partnering with chemists to develop fluorescent «labels» that can pinpoint the location of cancerous cells in the brain.

Close - up of muscle fibres in a zebrafish with the protein, actin, labelled with green fluorescent protein (GFP) Image credit: Elisabeth Busch, Wellcome Trust Sanger Institute

He used a fluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likfluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likFluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - like activity.

Because the embryo is transparent it is possible to use fluorescent proteins to label specific cells and their components, and then use this to watch the fate of the cells in the presence or absence of different genes.

We develop methods to label RNA in fixed and living cells using fluorescent probes and microscopy techniques and image analysis algorithms to visualize and quantify many mRNAs simultaneously.

BDA was detected by streptavidine labeled with fluorescent dye Alexa 555 (Molecular Probes Europe BV, Leiden, The Netherlands, 1 ∶ 400 in PBS - T).

Researchers at SciLifeLab / Uppsala University headed by Johan Elf have used a combination of microfluidics and image analysis of E. coli with fluorescent labels on the replication machinery to study how the replication and division cycles are coupled in individual cells.

For colocalization of both tracers or one tracer together with ZENK signals, primary antibodies (Egr - 1 / ZENK, 1 ∶ 500; CtB, 1 ∶ 300 in PBS - T) were detected by an appropriate secondary antibody (polyclonal goat raised against rabbit IgG labeled with fluorescent dyes Alexa488; Molecular Probes Europe BV, Leiden, The Netherlands, 1 ∶ 400 in PBS - T).

In early - stage oocytes oskar - TRICK mRNA is labeled by both NLS - PCP - GFP (green) and NLS - MCP - RFP (red), fluorescent RNA - binding proteins, indicating translational repression.

Nerve cells in the mouse brain that have been labeled with a modified rabies virus and fluorescent proteins.