Sentences with phrase «fluorescent labels of»

But scientists say that it has potential for even better resolution and for examining protein - protein interactions, particularly if fluorescent labels of different colors can be applied to proteins.

A general method for site specific fluorescent labeling of recombinant chemokines.

about Stable Fluorescent Labelling of human iPSCs aid Cell Tracking after Differentiation and Transplantation: Strategies and Pitfalls!

Generation, purification, and testing of primary antibodies Gold -, enzyme - and fluorescent labeling of secondary antibodies In situ hybridization Light - and electron microscopy

Not exact matches

While light - sheet microscopy is an old idea — scientists at ZEISS Microscopy and collaborators first came up with it in 1903 — only in this century has the convergence of fluorescent labels that work to process image volumes combined to make light - sheet mainstream.

The system uses an enzyme called DNA polymerase to read a strand of DNA and build a complementary strand out of nucleotides labeled with fluorescent molecules.

Using fluorescent labels, they were able to track the fates of marked and unmarked chromosomes under the microscope, from egg cells and sperm to the dividing cells of embryos after fertilization.

Developmental biologist Francesca Peri of the European Molecular Biology Laboratory in Heidelberg, Germany, and colleagues created genetically engineered versions of the animals that produced microglia labelled with green fluorescent protein, a glowing compound frequently used in laboratory research.

Generic labeling of proteins is achieved through fluorescent - antibody binding.

In order to test the sensitivity and specificity of spectral histopathology, the RUB team compared SHP with classical immunohistochemical methods, in which tumours are identified with the aid of fluorescent labels.

One side of each dumbbell was filled with a different short sequence of DNA labeled with a fluorescent molecule.

Due to the usage of lateral displacement, the nano - biomolecules can be detected in real - time and the detection is significantly faster in comparison to fluorescent label based detection.

This novel fluorescent label - free approach uses the lateral shifts in the position of the microbead substrate in pillar arrays, for quantifying the biomolecules, based on the change in surface forces and size, without the need of any external equipment.

The group employed various viral tracing methods — infecting receptor - expressing neurons with a virus strain and watching them spread as they label infected cells with a fluorescent protein — to visualize the neural circuit downstream of the ESP1 receptor, as well as providing an image of nerve fibers belonging to specific neurons in the brain and synapses relaying impulses from neuron to neuron, to map the anatomical foundation that conveys ESP1 signals in the brain.

Typically, detection of biomolecules such as proteins are performed using colorimetric assays or fluorescent labelling with a secondary antibody for detection, and requires complex optical detection equipment such as fluorescent microscopy or spectrophotometry.

Biologists at the University of Cambridge used fluorescent labelling to stain cell membranes in skate embryos, and tracked them through the dynamic development process.

The group of imaging specialists led by Prof. Michael Schäfers, Coordinator of the Cluster of Excellence, labelled the cells thus obtained with various fluorescent dyes in order to be able to study them in living organisms — initially with the optical method of fluorescence reflectance imaging.

Instead, the new method, called patterned photoactivation non-linear SIM, begins by switching on just a subset of fluorescent labels in a sample with a pattern of light.

By labeling the different subgroups with fluorescent dyes, researchers determined that just one of the subclones was responsible for the disease metastasis.

To generate an image, all of the fluorescent labels in a protein are switched on, then a wave of light is used to deactivate most of them.

They removed spermatogonia labeled with a fluorescent marker from a mature male trout and injected the cells into the abdomens of newly hatched male and female trout fry.

Sandia National Laboratories researchers are drawing inspiration from neurons in the brain, such as these green fluorescent protein - labeled neurons in a mouse neocortex, with the aim of developing neuro - inspired computing systems to reboot computing.

Specifically, cell cultures from bone tissue were incubated in 10 % DMEM containing 10 ng / ml TNF - α and 10 μg / ml fluorescent probe of acetylated LDL, DiI - Ac - LDL (Biochemical Technologies, Cambridge, MA), for 4 h. Cells were harvested in the manner described above with the exception that during this labeling period, the rat anti-E-selectin mAb (10E9.6) conjugated to FITC replaced PE - conjugated 10E9.6.

They found that injecting into the carotid artery breast cancer cells that express markers allowing them to enter the brain — cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.

We have placed fluorescent labels on portions of the tail regions of Arabidopsis myosin XIs in order to determine whether they localize, and therefore what role a particular myosin may play in movement of different types of organelles.

«We trained the neural network by showing it two sets of matching images of the same cells; one unlabeled and one with fluorescent labels,» explained Christiansen, software engineer at Google Accelerated Science.

By labeling one of the shell proteins with Yellow Fluorescent Protein (YFP), we were able to use confocal fluorescence microscopy to observe punctuate loci forming inside chloroplasts.

The same principle lies behind the powerful and visually spectacular Brainbow cell - labeling technique, in which each labeled cell expresses a unique ratio of three or four distinct colors of fluorescent protein.

The membranes of cells that have been successfully targeted with gesicles are transiently labeled with red fluorescent protein.

One common complaint about using four - color Confetti to label single cells (in an animal) is that the cells express green fluorescent protein less often, or sometimes not at all, compared with cells that express each of the other three colors.

«We trained the neural network by showing it two sets of matching images of the same cells; one unlabeled and one with fluorescent labels,» coauthor Eric Christiansen, a software engineer at Google Accelerated Science, says a press release.

Most colocalization methods are based on pixel overlap between the previously denoised signal that is emitted from two (or more) different fluorescent labels, and use a global image correlation such as Pearson's of Manders» coefficients.

The authors then tested the tumor targeting capability of iPSC - EPCs labelled with the near - infrared fluorescent dye DiR in two established mouse breast cancer models.

Quantitative microscopy of green fluorescent protein - labeled yeast.

Briefly, 15 μl of 100 nm diameter rhodamine - labelled fluorescent latex microsphere particles (LumaFluor Corp, Naples, USA) in 200 μl NaCl solution were injected i.v. 24 h before primary tumour removal.

PromoKine offers a wide range of well proven products for cell biology research such as kits and reagents for cell analysis, apoptosis research, cell transfection, fluorescent labeling, gene cloning & expression, mycoplasma detection and elimination as well as numerous antibodies, ELISAs, cytokines and growth factors.

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

In 2002, an offer to join the faculty of MIT's Chemistry Department drew her back to the East Coast, where she was able to begin tackling the problem that had «repeatedly stymied» her throughout her PhD and postdoc years: site - specific protein labeling — how to label a particular protein with a fluorescent tag or other «chemical probe» to reveal information about that protein's function.

(Right three images) Labeled images created with three different fluorescent labels, revealing invisible details of cell nuclei (blue), dendrites (green), and axons (red).

Neurosurgeons are partnering with chemists to develop fluorescent «labels» that can pinpoint the location of cancerous cells in the brain.

For example, with standard fluorescent microscope filter sets, users can label multiple proteins of interest with a combination of green and blue or cyan and yellow moxFPs.

To achieve even cell labeling, they generated farnesylated derivatives of the fluorescent proteins, which are directly trafficked to cell membranes.

Close - up of muscle fibres in a zebrafish with the protein, actin, labelled with green fluorescent protein (GFP) Image credit: Elisabeth Busch, Wellcome Trust Sanger Institute

He used a fluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likfluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likFluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - like activity.

Because the embryo is transparent it is possible to use fluorescent proteins to label specific cells and their components, and then use this to watch the fate of the cells in the presence or absence of different genes.

T - tubules of isolated cardiomyocytes from rodent models of cardiac diseases are labelled using fluorescent dextran that diffuses from extracellular space to TATS lumen.

Neuron expressing a fluorescent protein engineered by the Looger Lab was reconstructed from immunogold labeled serial sections of the mouse brain.

Researchers at SciLifeLab / Uppsala University headed by Johan Elf have used a combination of microfluidics and image analysis of E. coli with fluorescent labels on the replication machinery to study how the replication and division cycles are coupled in individual cells.

For colocalization of both tracers or one tracer together with ZENK signals, primary antibodies (Egr - 1 / ZENK, 1 ∶ 500; CtB, 1 ∶ 300 in PBS - T) were detected by an appropriate secondary antibody (polyclonal goat raised against rabbit IgG labeled with fluorescent dyes Alexa488; Molecular Probes Europe BV, Leiden, The Netherlands, 1 ∶ 400 in PBS - T).

Such an optical contrast exceeded the optical contrast of the fluorescent labels (that were targeted to C4 - 2B and HS - 5 cells using the same prostate cancer - specific PSMA antibody, see Figure 7B, D) by 31 times.