Researchers at SciLifeLab / Uppsala University headed by Johan Elf have used a combination of microfluidics and image analysis of E. coli with
fluorescent labels on the replication machinery to study how the replication and division cycles are coupled in individual cells.
We have placed
fluorescent labels on portions of the tail regions of Arabidopsis myosin XIs in order to determine whether they localize, and therefore what role a particular myosin may play in movement of different types of organelles.
Not exact matches
This novel
fluorescent label - free approach uses the lateral shifts in the position of the microbead substrate in pillar arrays, for quantifying the biomolecules, based
on the change in surface forces and size, without the need of any external equipment.
Using new technologies for
labeling cellular machinery with light - activated
fluorescent markers, they could «turn
on» just one molecule at a time.
In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the technique works
on tissue that has been previously
labeled with either
fluorescent antibodies or proteins such as green
fluorescent protein (GFP).
Green
fluorescent protein
labeling allowed them to see the early development pattern and show that lncND, which ordinarily is not present in mice — lncND is present only in some primates including humans — had a functional effect
on development.
This latter capability is extremely valuable to biomedical research, which relies heavily
on fluorescent probes to
label and track proteins, nucleic acids, and other cellular components.
Instead, the new method, called patterned photoactivation non-linear SIM, begins by switching
on just a subset of
fluorescent labels in a sample with a pattern of light.
To generate an image, all of the
fluorescent labels in a protein are switched
on, then a wave of light is used to deactivate most of them.
Most colocalization methods are based
on pixel overlap between the previously denoised signal that is emitted from two (or more) different
fluorescent labels, and use a global image correlation such as Pearson's of Manders» coefficients.
For the visitors, seeing a tiny embryo down a microscope was certainly interesting, but when the
fluorescent light was turned
on, revealing such things as red -
labelled blood flowing through glowing green vessels, the exclamation heard was usually «wow»!
Using
fluorescent labels that switch
on and off, researchers activate and deactivate
fluorescent molecules in specific regions at specific times.
Conventional flow cytometry is a powerful technique for measuring cell phenotype and function, but it relies
on fluorescent stains, or
labels, to identify particular cell subpopulations.