Sentences with phrase «fluorescent marker»

Next, ovarian cancer cells, expressing a fluorescent marker to distinguish them from the other cells, were added.
Wiedenmann, Jörg, et al. «EosFP, a fluorescent marker protein with UV - inducible green - to - red fluorescence conversion.»
He used a fluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - like activity.
A cortical layer 2/3 neuron at 21 days in vitro labeled by ex utero electroporation with fluorescent marker for spine visualization.
Cortical neurons from Prof. Studer's lab that were derived from human pluripotent stem cells visualized with a fluorescent marker indicating their identity.
We recommend confirming the presence of the transgene by PCR or examination of the cell line microscopically for fluorescent marker expression.
The provirus in the cell lines also encoded a fluorescent marker to illuminate HIV - 1 transcriptional activity.
However, we have found a fluorescent marker for nestin, a neural crest stem cell marker, in the posterior limbal region of the human anterior segment in the vicinity of both the trabecular meshwork and Schwalbe's line (unpublished).
Viewed through the glasses, cancer cells appear to glow blue under a special light, thanks to a fluorescent marker injected in the tumor that attaches only to cancerous and not to healthy cells.
Additionally, the latent provirus integrated in the J - Lat cell lines encodes the GFP gene, providing a fluorescent marker of HIV - 1 transcriptional activity.
The oncogene was coupled with a fluorescent marker so that cells in which the oncogene was active could be easily identified, and as these cells proliferate, their «daughter» cells could also be tracked.
Nine weeks after receiving the injections, long after the animal was born, the scientists examined its brain to see where the cells, which they'd labeled with a fluorescent marker, had gone.
The chemists built a detector with modified potato tissue supported in a reactor vessel containing a fluorescent marker.
It uses a specially developed fluorescent marker which attaches to cell proteins when injected into patients.
They removed spermatogonia labeled with a fluorescent marker from a mature male trout and injected the cells into the abdomens of newly hatched male and female trout fry.
Using L1 tagged with a fluorescent marker, the group observed higher numbers of fluorescent iPSCs from non-human primates compared with humans.
He and his colleagues developed a fluorescent marker for early - stage germ cells, cells that eventually turn into sperm or eggs.
These cells, glowing green due to a fluorescent marker indicating their stem cell nature, were reportedly made through a new method that has been called into question.
By adding a fluorescent marker to the neurons, researchers could watch the pair light up when exposed to magnetic fields.
Scientists at The Institute of Cancer Research (ICR) used the cells of fruit flies, modified with a fluorescent marker that is activated when the cells are put under stress, to identify the signals responsible for driving up activity of the ER.
The mice were a strain previously engineered to produce a bright green fluorescent marker in any active neurons.
However, it takes a very long time for the fluorescent marker to spread evenly into peripheral blood cells, an amount of time that even exceeds the lifespan of a mouse.
This fluorescent marker can be turned on at any time by administering a specific reagent to the animal.
Blood stem cells in the bone marrow have been labeled with a fluorescent marker protein.
Scientists from the German Cancer Research Center (DKFZ) have developed a way to equip mouse blood stem cells with a fluorescent marker that can be switched on from the outside.
He proved it in 2003 by first X-raying mice to kill off all the stem cells in their bone marrow, then repopulating the bone with stem cells tagged with a fluorescent marker.
To track activity, Lu's team included a fluorescent marker
Even fluorescent markers will work if you dare to bring them out of hiding.
The PCR reactions occur inside the individual droplets, which the machine then sorts with fluorescent markers to detect target sequences.
The first step involves tagging target sequences with fluorescent markers.
After watching those mice thrive for several months, Lagasse repeated the experiment using fluorescent markers to trace the path of the liver cells.
Fluorescent markers injected into the transparent brains can highlight the RNA in its original position.
Depending on which device they buy, researchers can photograph cells at anywhere from 20x to 60x magnification, and use the included software to sort them by purely visual characteristics such as shape and size, fluorescent markers, or both.
Equipped with a robotic arm and surgical tools, STAR combines smart imaging technologies and fluorescent markers to navigate and adapt to the complexities of soft tissue.
Kelley and his team also used mice with fluorescent markers in different cells of the ear followed by next generation sequencing.
Fluorescent markers combined with the reagents are automatically read by a microscope to determine the precise concentrations in each droplet and also observe how the reaction proceeds.
Using fluorescent markers, Fuchs and her colleagues isolated two distinct populations of stem cells from mouse skin.
Using new technologies for labeling cellular machinery with light - activated fluorescent markers, they could «turn on» just one molecule at a time.
«This methodology is very important because it allows you to very quickly integrate almost any piece of DNA in these vectors,» remarks Bellen, who says this technology also allows scientists to put single point mutations in a gene and then reinsert the gene into a genome as well as tagging a segment of DNA with fluorescent markers.
By tagging DNA strands with fluorescent markers, each chain could be observed in detail as it was stretched.
And by using fluorescent markers to tag these cells, they have even been able to detect them by using a simple mobile phone camera.
The most common probes, which use fluorescent markers or radioactive labels to pinpoint the virus's location in a tissue sample, sometimes have difficulty distinguishing the target — HIV RNA and DNA — from surrounding cellular components.
Now, researchers at the University of Cambridge have used fluorescent markers to track cell development in the embryo of a cartilaginous fish — a little skate in this case — and found that these thorny scales are in fact created from the same type of cells as teeth: neural crest cells.
Fluorescent markers reveal that this Medfly contains genes that make it sterile.
«Here we have demonstrated the further use of this novel technology to develop highly sought after biologically relevant fluorescent markers quickly and easily for different imaging needs,» says Caldwell, who led the study.
They found that injecting into the carotid artery breast cancer cells that express markers allowing them to enter the brain — cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.
The injected cells express markers that allow them to enter the brain and are labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies.
So Gaddis used mice in which regulatory T cells would be tagged with two fluorescent markers.
«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.
Use fluorescent markers as a guide for blot - film orientation.
a b c d e f g h i j k l m n o p q r s t u v w x y z