Sentences with phrase «fluorescent marker in»

The mice were a strain previously engineered to produce a bright green fluorescent marker in any active neurons.

Kelley and his team also used mice with fluorescent markers in different cells of the ear followed by next generation sequencing.

Not exact matches

C. elegans with a green fluorescent protein (GFP) marker in the ASH neuron, the neuron that senses high pH.

Fluorescent markers injected into the transparent brains can highlight the RNA in its original position.

He proved it in 2003 by first X-raying mice to kill off all the stem cells in their bone marrow, then repopulating the bone with stem cells tagged with a fluorescent marker.

Blood stem cells in the bone marrow have been labeled with a fluorescent marker protein.

Fluorescent markers combined with the reagents are automatically read by a microscope to determine the precise concentrations in each droplet and also observe how the reaction proceeds.

The researchers developed a genetic marker that gives off fluorescent light in the presence of manatee DNA.

Fluorescent dyes, which are frequently used as markers in biological experiments, are particularly hard to detect in Raman spectroscopy because the fluorescence tends to wash out the signal.

«This methodology is very important because it allows you to very quickly integrate almost any piece of DNA in these vectors,» remarks Bellen, who says this technology also allows scientists to put single point mutations in a gene and then reinsert the gene into a genome as well as tagging a segment of DNA with fluorescent markers.

By tagging DNA strands with fluorescent markers, each chain could be observed in detail as it was stretched.

To find out if this was true, workers in stem - cell biologist Irving Weissman's lab at Stanford University Medical School took one blood stem cell from an adult mouse and tagged it with a marker that glowed green under fluorescent light.

A team of researchers from DuPont added so - called morphogenic genes — known from basic research to promote the production of embryonic tissue — to the other genes being transformed (in this case to express green fluorescent protein as a marker of transformation).

Invaluable as markers for monitoring photosynthesis and other energy - related processes in living cells, green fluorescent proteins (GFPs), discovered in a species of jellyfish, are vital in extremely high - resolution imaging studies.

The most common probes, which use fluorescent markers or radioactive labels to pinpoint the virus's location in a tissue sample, sometimes have difficulty distinguishing the target — HIV RNA and DNA — from surrounding cellular components.

* She combined a marker — a gene for a jellyfish protein that fluoresces green — with a DNA sequence that turns on the green fluorescent protein in the presence of thyroid hormones.

Now, researchers at the University of Cambridge have used fluorescent markers to track cell development in the embryo of a cartilaginous fish — a little skate in this case — and found that these thorny scales are in fact created from the same type of cells as teeth: neural crest cells.

The chemists built a detector with modified potato tissue supported in a reactor vessel containing a fluorescent marker.

The scientists were thus able to transfer the genetic information from the known fluorescent protein eqFP615 into the DT40 chicken B cell line in order to produce protein variants of the new infrared marker Amrose with different spectral properties.

The oncogene was coupled with a fluorescent marker so that cells in which the oncogene was active could be easily identified, and as these cells proliferate, their «daughter» cells could also be tracked.

Additionally, the latent provirus integrated in the J - Lat cell lines encodes the GFP gene, providing a fluorescent marker of HIV - 1 transcriptional activity.

They found that injecting into the carotid artery breast cancer cells that express markers allowing them to enter the brain — cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.

Finally, pathogens will be tagged with fluorescent / bioluminescent probes or will be genetically modified to express markers for detection by in vivo imaging, so that dissemination and proliferation can be visualised in vaccinated and challenged animals.

Viewed through the glasses, cancer cells appear to glow blue under a special light, thanks to a fluorescent marker injected in the tumor that attaches only to cancerous and not to healthy cells.

However, we have found a fluorescent marker for nestin, a neural crest stem cell marker, in the posterior limbal region of the human anterior segment in the vicinity of both the trabecular meshwork and Schwalbe's line (unpublished).

The provirus in the cell lines also encoded a fluorescent marker to illuminate HIV - 1 transcriptional activity.

So Gaddis used mice in which regulatory T cells would be tagged with two fluorescent markers.

Fluorescent and luminescent materials, such as yttrium aluminum garnet doped with different metal ions (rare earths or transition metals), have found applicability in various fields, from optoelectronics (i.e.: lighting systems, LCDs, CRTs, lasers) to aerospace (i.e: thermal barriers) and biotechnological applications (i.e.: fluorescence marker, contrast agent in medical imaging, imaging screens, drug delivery etc).

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

A cortical layer 2/3 neuron at 21 days in vitro labeled by ex utero electroporation with fluorescent marker for spine visualization.

He used a fluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likfluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likFluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - like activity.

Pharmacology Assays Fluorescent or luminescent markers are also used in pharmacological and drug discovery assays.

In this technique, antibodies against distemper virus are tagged with fluorescent markers.