Sentences with phrase «fluorescent markers of»

Additionally, the latent provirus integrated in the J - Lat cell lines encodes the GFP gene, providing a fluorescent marker of HIV - 1 transcriptional activity.

Not exact matches

Even fluorescent markers will work if you dare to bring them out of hiding.

After watching those mice thrive for several months, Lagasse repeated the experiment using fluorescent markers to trace the path of the liver cells.

However, it takes a very long time for the fluorescent marker to spread evenly into peripheral blood cells, an amount of time that even exceeds the lifespan of a mouse.

Equipped with a robotic arm and surgical tools, STAR combines smart imaging technologies and fluorescent markers to navigate and adapt to the complexities of soft tissue.

Kelley and his team also used mice with fluorescent markers in different cells of the ear followed by next generation sequencing.

Using fluorescent markers, Fuchs and her colleagues isolated two distinct populations of stem cells from mouse skin.

The researchers developed a genetic marker that gives off fluorescent light in the presence of manatee DNA.

«This methodology is very important because it allows you to very quickly integrate almost any piece of DNA in these vectors,» remarks Bellen, who says this technology also allows scientists to put single point mutations in a gene and then reinsert the gene into a genome as well as tagging a segment of DNA with fluorescent markers.

Scientists at The Institute of Cancer Research (ICR) used the cells of fruit flies, modified with a fluorescent marker that is activated when the cells are put under stress, to identify the signals responsible for driving up activity of the ER.

A team of researchers from DuPont added so - called morphogenic genes — known from basic research to promote the production of embryonic tissue — to the other genes being transformed (in this case to express green fluorescent protein as a marker of transformation).

Invaluable as markers for monitoring photosynthesis and other energy - related processes in living cells, green fluorescent proteins (GFPs), discovered in a species of jellyfish, are vital in extremely high - resolution imaging studies.

* She combined a marker — a gene for a jellyfish protein that fluoresces green — with a DNA sequence that turns on the green fluorescent protein in the presence of thyroid hormones.

Using L1 tagged with a fluorescent marker, the group observed higher numbers of fluorescent iPSCs from non-human primates compared with humans.

Now, researchers at the University of Cambridge have used fluorescent markers to track cell development in the embryo of a cartilaginous fish — a little skate in this case — and found that these thorny scales are in fact created from the same type of cells as teeth: neural crest cells.

They removed spermatogonia labeled with a fluorescent marker from a mature male trout and injected the cells into the abdomens of newly hatched male and female trout fry.

The scientists were thus able to transfer the genetic information from the known fluorescent protein eqFP615 into the DT40 chicken B cell line in order to produce protein variants of the new infrared marker Amrose with different spectral properties.

«Here we have demonstrated the further use of this novel technology to develop highly sought after biologically relevant fluorescent markers quickly and easily for different imaging needs,» says Caldwell, who led the study.

They found that injecting into the carotid artery breast cancer cells that express markers allowing them to enter the brain — cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.

However, we have found a fluorescent marker for nestin, a neural crest stem cell marker, in the posterior limbal region of the human anterior segment in the vicinity of both the trabecular meshwork and Schwalbe's line (unpublished).

We recommend confirming the presence of the transgene by PCR or examination of the cell line microscopically for fluorescent marker expression.

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

He used a fluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likfluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likFluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - like activity.

SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker -LRB-

SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr - unc - 119) for easy screening of the insertion event, and a variety of connector modules (linker sequences between the tag and homology arms).

This reporter employs a tandem array of internal ribosomal entry sites to drive translation of an enhanced Yellow Fluorescent Protein (Venus) from the transcript that normally encodes for the early endodermal marker Hex.

Fluorescence - based assays Fluorescent markers such as EGFP, YFP, mCherry and mTomato or fluorescent biosensors can be used to measure a variety of real - time cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and tFluorescent markers such as EGFP, YFP, mCherry and mTomato or fluorescent biosensors can be used to measure a variety of real - time cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and tfluorescent biosensors can be used to measure a variety of real - time cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and translation.

A gene for green fluorescent protein from the jellyfish, Aequorea Victoria, has been inserted into the cats» DNA and is used as a marker for cells carrying a second gene which confers resistance to the feline version of the AIDS virus, the Feline Immunodeficiency Virus (FIV).

STAR uses advanced computer vision tech that uses fluorescent markers and 3D cameras to get a detailed view of the surgical site.