Not exact matches
To determine whether their method had succeeded, the researchers inserted plasmids carrying a gene that
codes for a green
fluorescent protein.
The new AAV vectors can also deliver genes that
code for colorful
fluorescent proteins; such
proteins are useful in identifying and labeling cells.
Imaging techniques that rely on light — such as taking pictures of cells tagged with a «reporter gene» that
codes for green
fluorescent protein — only work in tissue samples removed from the body.
Masaru Okabe, a biologist at Osaka University in Japan, engineered these green mice by injecting into mouse embryos a jellyfish gene that
codes for a glowing
protein called green
fluorescent protein.
Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual neurons in the brain, to label cells stochastically using gene cassettes that
code for four uniquely expressed
fluorescent proteins.
CRISPR / Cas9 and related tools can now be used in new ways, such as changing a single nucleotide base — a single letter in the genetic
code — or adding a
fluorescent protein to tag a spot in the DNA that scientists want to track.
The kit includes lyophilized Xfect Transfection Reagent and gesicle packaging mixes which contain
coding sequences for the nanovesicle - inducing glycoprotein, Cas9 endonuclease, and CherryPicker red
fluorescent protein.
For the purpose of their study, the researchers inserted a gene
coding for
fluorescent protein in the worms that, when activated, made them glow brightly under ultraviolet light.
The remaining cassette can invert as long as Cre remains expressed; once Cre is removed from the system, the
fluorescent protein whose
coding sequence is closest to the promoter will be expressed.
To accomplish this, we used homologous recombination to ablate nonmuscle myosin heavy chain (NMHC) II - B by inserting cDNA encoding green
fluorescent protein (GFP)- NMHC II - A into the first
coding exon of the Myh10 gene, thereby placing GFP - NMHC II - A under control of the endogenous II - B promoter.
Probing unnatural amino acid integration into enhanced green
fluorescent protein by genetic
code expansion with a high - throughput screening platform.