A video shows a transparent larva's body as Cryptococcus fungi, made red by
a fluorescent tracer, moves through tissue, blood vessels and into the brain.
For colocalization of both
tracers or one
tracer together with ZENK signals, primary antibodies (Egr - 1 / ZENK, 1 ∶ 500; CtB, 1 ∶ 300 in PBS - T) were detected by an appropriate secondary antibody (polyclonal goat raised against rabbit IgG labeled with
fluorescent dyes Alexa488; Molecular Probes Europe BV, Leiden, The Netherlands, 1 ∶ 400 in PBS - T).