Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using
fluorescently labeled antibodies.
Target bacteria were captured using antibody - coated magnetic beads and then encapsulated in picoliter - sized droplets with
fluorescently labeled antibodies for detection.
To determine pluripotency and AcGFP1 knockout efficiency in gesicle - treated cells, cells were labeled with
a fluorescently labeled antibody specific to the pluripotency marker SSEA - 4.
Not exact matches
Immunoreactions were developed using a
fluorescently labeled secondary
antibody, and samples visualized in a Leica Biosystems epifluorescent microscope or using a secondary
antibody linked to horseradish peroxidase and developed with a 3,3 ′ - diaminobenzidine kit.
The SVCs were incubated with a
fluorescently labeled anti-F4 / 80
antibody and were sorted by FACS into F4 / 80 - expressing (F4 / 80 +) and - nonexpressing (F4 / 80 ---RRB- populations.
Frozen sections of affected tissues were then stained with
fluorescently -
labeled Borrelia - specific polyclonal
antibodies.
Immunofluorescent
labeling was performed using anti-Olig2 (1 ∶ 4000, Chemicon) and anti-GFAP (1 ∶ 400, Cell Signaling) followed by
fluorescently labeled, secondary anti-Ig
antibodies (Alexa 488 and 568 conjugates, Invitrogen) at a 1 ∶ 2000 dilution.
We make use of
fluorescently -
labeled antibodies and FACS machines to sort specific cellular populations.
HuProt Cross-Section: microarray probed with a serum Ig sample and
fluorescently -
labeled secondary
antibodies to the Ig and common epitopes of the arrayed proteins.
Rat astrocytes stained with
fluorescently labelled Caveolin - 1
antibody.